US3879510A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Modification of red blood cell membrane with polyisocyanates
Assignee: Fujifilm Holdings Corp
•
Filing: 1972-04-11
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Granted: 1975-04-22
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Expires: 1992-04-11
Abstract
Abstract A method of modifying red blood cells, which comprises: ADDING A POLYISOCYANATE IN THE FORM OF AN OIL-IN-WATER EMULSION CONSISTING ESSENTIALLY OF A POLYISOCYANATE AND A SALINE SOLUTION TO A SALINE SUSPENSION OF RED BLOOD CELLS, SAID EMULSION HAVING BEEN PREPARED AT A TEMPERATURE NOT EXCEEDING 35*C., and within a period of time approximating 5 minutes.
Inventors
- 👤 Masao Kitajima
- 👤 Asaji Kondo
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:50
CPC Classifications
A; A61; A61K; A61K51/00; A61K51/12; A61K51/1203; A61K2123/00; Y; Y10; Y10S; Y10S436/00; Y10S436/826
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
127 words
A METHOD FOR MODIFYING RED BLOOD CELLS BY REINFORCING THE MEMBRANES THEREOF WITHOUT DENATURING THEIR HEMOGLOBIN WHICH COMPRISES: ADDING A POLYISCYANATE SELECTED FROM THE GROUP CONSISTING OF TOLUYLENE DIISOCYANATE AND TRIPHENYLEMETHANE TRIISOCYANATE IN THE FORM OF AN EMULSION OF SAID POLYISOCYANATE IN WATER TO A SALINE SUSPENSION OF RED BLOOD CELLS, SAID EMULSION HAVING BEEN PREPARED AT A TEMPERATURE NOT EXCEEDING 35*C., AND WITHIN A PERIOD OF TIME APROXIMATING 5 MINUTES; THEREBY PRODUCING SAID MODIFIED RED BLOOD CELLS CHARACTERIZED BY REINFORCED MEMBRANES AND HEMOGLOVIN RESISTENT TO DENATURING; SEPARATING SAID MODIFIED RED BLOOD CELLS FROM THE REMAINDER OF SAID SALINE SUSPENSION AND COLLECTING SAID MODIFIED RED BLOOD CELLS, SAID POLYISOCYANATE BEING PRESENT IN AN AMOUNT OF 10**-4 TO 10**-1 TIMES BY WEIGHT OF THE RED BLOOD CELLS PRESENT.
Claim 2
Independent Claim
127 words
A METHOD FOR MODIFYING RED BLOOD CELLS BY REINFORCING THE MEMBRANES THEREOF WITHOUT DENATURING THEIR HEMOGLOBIN WHICH COMPRISES: ADDING A POLYISCYANATE SELECTED FROM THE GROUP CONSISTING OF TOLUYLENE DIISOCYANATE AND TRIPHENYLEMETHANE TRIISOCYANATE IN THE FORM OF AN EMULSION OF SAID POLYISOCYANATE IN WATER TO A SALINE SUSPENSION OF RED BLOOD CELLS, SAID EMULSION HAVING BEEN PREPARED AT A TEMPERATURE NOT EXCEEDING 35*C., AND WITHIN A PERIOD OF TIME APROXIMATING 5 MINUTES; THEREBY PRODUCING SAID MODIFIED RED BLOOD CELLS CHARACTERIZED BY REINFORCED MEMBRANES AND HEMOGLOVIN RESISTENT TO DENATURING; SEPARATING SAID MODIFIED RED BLOOD CELLS FROM THE REMAINDER OF SAID SALINE SUSPENSION AND COLLECTING SAID MODIFIED RED BLOOD CELLS, SAID POLYISOCYANATE BEING PRESENT IN AN AMOUNT OF 10**-4 TO 10**-1 TIMES BY WEIGHT OF THE RED BLOOD CELLS PRESENT.
Claim 3
Independent Claim
129 words
A method for modifying red blood cells by reinforcing the membranes thereof without denaturing their hemoglobin which comprises: adding a polyisocyanate selected from the group consisting of toluylene diisocyanate and triphenylmethane triisocyanate in the form of an emulsion of said polyisocyanate in water to a saline suspension of red blood cells, said emulsion having been prepared at a temperature not exceeding 35*C., and within a period of time approximating 5 minutes; thereby producing said modified red blood cells characterized by reinforced membranes and hemoglobin resistent to denaturing; separating said modified red blood cells from the remainder of said saline suspension and collecting said modIfied red blood cells, said polyisocyanate being present in an amount of 10 4 to 10 1 times by weight of the red blood cells present.
Claim 4
Independent Claim
129 words
A method for modifying red blood cells by reinforcing the membranes thereof without denaturing their hemoglobin which comprises: adding a polyisocyanate selected from the group consisting of toluylene diisocyanate and triphenylmethane triisocyanate in the form of an emulsion of said polyisocyanate in water to a saline suspension of red blood cells, said emulsion having been prepared at a temperature not exceeding 35*C., and within a period of time approximating 5 minutes; thereby producing said modified red blood cells characterized by reinforced membranes and hemoglobin resistent to denaturing; separating said modified red blood cells from the remainder of said saline suspension and collecting said modIfied red blood cells, said polyisocyanate being present in an amount of 10 4 to 10 1 times by weight of the red blood cells present.
Claim 5
Dependent Claim
12 words
The modified red blood cells produced by the method of claim 1.
Claim 6
Dependent Claim
11 words
The method of claim 1, wherein said polyisocyanate is toluylene diisocyanate.
Claim 7
Dependent Claim
11 words
The method of claim 1 wherein the polyisocyanate is triphenylmethane triisocyanate.
Claim 8
Dependent Claim
22 words
The method of claim 1, wherein the polyisocyanate is dissolved in a substantially hydrophobic organic solvent, immiscible with water prior to emulsification.
Claim 9
Dependent Claim
15 words
The method of claim 1, wherein said saline suspension of red blood cells is isotonic.
Claim 10
Dependent Claim
15 words
The method of claim 1, wherein said saline suspension of red blood cells is hypertonic.
Claim 11
Dependent Claim
22 words
The method of claim 1, wherein said polyisocyanate emulsion contains from 0.001 to 0.1% based on said emulsion of a suitable stabilizer.
References Cited (Backward Citations)
US3639558A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-04-21 | 1978-10-21 | 1979-04-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-04-21 | 1982-10-21 | 1983-04-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-04-21 | 1986-10-21 | 1987-04-22 | $7700.00 | Window Closed / Lapsed |