US3876501A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Binding enzymes to activated water-soluble carbohydrates
Assignee: Baxter International Inc
•
Filing: 1973-05-17
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Granted: 1975-04-08
•
Expires: 1993-05-17
Abstract
Abstract Water-soluble carbohydrates can be activated by means of cyanogen bromide in a nonaqueous medium to exhibit an improved level of binding reactivity toward enzymes and other proteins, while preventing cross-linking and consequent water-insolubility in the carbohydrates.
Inventors
- 👤 Michael Hanushewsky
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 06:35
CPC Classifications
C; C08; C08B; C08B31/00; C08B37/00; C12; C12N; C12N11/00; C12N11/02; C12N11/10; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/813
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
145 words
A METHOD OF ACTIVITING A WATER-SOLUBLE CARBOHYDRATE AND CHEMICALLY BINDING IT WITH ENZYME MOLECULES, WHICH COMPRISES: COMBINING SAID CARBOHYCRATE WITH A CYANOGEN HALIDE IN THE PRESENCE OF A LOWER ALCOHOL ESSENTIALLY FREE OF WATER SELECTED FROM THE GROUP CONSISTING OF METHANOL, ETHANOL, PROPANOL AND BUTANOL, AND IN THE PRESENCE OF A DISSOLVED ALKALI OF THE FORMULA MOR IN A CONCENTRATION SUFFICIENT TO PROVIDE A PH OF AT LEAST 10 IF THE SAME ALKALI CONCENTRATION WERE ADDED TO DEIONIZED WATER, IN WHICH M IS AN ALKALI METAL AND R IS SELECTED FROM THE GROUP CONSISTING OF METHYL, ETHYL, PROPYL AND BUTYL RADICALS FOR A PERIOD OF TIME SUFFICIENT TO PERMIT SAID ACTIVATION OF THE CARBOHYDRATE, AND AFTER SAID PERIOD OF TIME BRINGING FROM 1 TO 300 PARTS BY WEIGHT OF SAID ACTIVATED CARBOHYDRATE INTO CONTACT WITH ONE PART BY WEIGHT OF AN ENZYME FOR REACTION THEREBETWEEN.
Claim 2
Independent Claim
145 words
A METHOD OF ACTIVITING A WATER-SOLUBLE CARBOHYDRATE AND CHEMICALLY BINDING IT WITH ENZYME MOLECULES, WHICH COMPRISES: COMBINING SAID CARBOHYCRATE WITH A CYANOGEN HALIDE IN THE PRESENCE OF A LOWER ALCOHOL ESSENTIALLY FREE OF WATER SELECTED FROM THE GROUP CONSISTING OF METHANOL, ETHANOL, PROPANOL AND BUTANOL, AND IN THE PRESENCE OF A DISSOLVED ALKALI OF THE FORMULA MOR IN A CONCENTRATION SUFFICIENT TO PROVIDE A PH OF AT LEAST 10 IF THE SAME ALKALI CONCENTRATION WERE ADDED TO DEIONIZED WATER, IN WHICH M IS AN ALKALI METAL AND R IS SELECTED FROM THE GROUP CONSISTING OF METHYL, ETHYL, PROPYL AND BUTYL RADICALS FOR A PERIOD OF TIME SUFFICIENT TO PERMIT SAID ACTIVATION OF THE CARBOHYDRATE, AND AFTER SAID PERIOD OF TIME BRINGING FROM 1 TO 300 PARTS BY WEIGHT OF SAID ACTIVATED CARBOHYDRATE INTO CONTACT WITH ONE PART BY WEIGHT OF AN ENZYME FOR REACTION THEREBETWEEN.
Claim 3
Dependent Claim
13 words
The method of claim 1 in which said activated carbohydrate is in aqueous
Claim 4
Dependent Claim
13 words
The method of claim 2 in which said carbohydrate has a molecular weight
Claim 5
Dependent Claim
15 words
The method of claim 4 in which said solvent is an absolute alcohol of no
Claim 6
Dependent Claim
32 words
The method of claim 6 in which from 1 to 3 parts by weight of sodium methoxide, 1 to 3 parts by weight of dextran, and 1 to 3 parts by weight
Claim 7
Dependent Claim
12 words
The method of claim 5 in which a reaction temperature of from
Claim 8
Dependent Claim
16 words
The method of claim 1 in which from 10 to 80 parts by weight of said
Claim 9
Dependent Claim
13 words
The method of claim 12 in which said dissolved alkali is an alkali
Claim 10
Dependent Claim
12 words
The method of claim 13 in which said cyanogen halide is cyanogen
Claim 11
Dependent Claim
13 words
The method of claim 14 in which said dissolved alkali is sodium methoxide.
References Cited (Backward Citations)
US3645852A
Cited
US3788948A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-04-07 | 1978-10-07 | 1979-04-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-04-07 | 1982-10-07 | 1983-04-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-04-07 | 1986-10-07 | 1987-04-08 | $7700.00 | Window Closed / Lapsed |