Patent US3873433A successfully refreshed from Google Patents.
US3873433A
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Method of demonstrating normal and pathological plasm-lipoprotein patterns in human body liquids
Assignee: Oesterreichisches Institut fuer Haemoderivate
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Filing: 1973-12-19
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Granted: 1975-03-25
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Expires: 1993-12-19
Abstract
Abstract A method for the demonstration of normal and pathological plasmlipoprotein patterns in human body liquids by electrophoretical separation of the lipoproteins in a carrier medium, such as a gel, or on foils, which consists in that after the desired electrophoretical separation effect has been achieved the carrier is treated with a developing solution comprising polyanions, and an apparatus for carrying out the method.
Inventors
- 👤 Dietrich Seidel
- 👤 Heinrich Wieland
- 👤 Johann Eibl
- 👤 Gerald Eder
- 👤 Ewald Molinari
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 13:53
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44717; G01N27/44721; G01N27/44726
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
69 words
A METHOD OF DEMONSTRATING NORMAL AND PATHOLOGICAL PLASMA-LIPOPROTEIN PATTERNS IN HUMAN BODY LIQUIDS WHICH COMPRISES ELECTROPHORETICALLY SEPARATING LIPOPROTEINS IN A CARRIER MEDIUM OR ON FOILS, UPON OCCURRENCE OF A DESIRED ELECTROPHORETICAL SEPARATION EFFECT, TREATING THE CARRIER WITH A DEVELOPING SOLUTION, COMPRISING AT LEAST ONE OF THE FOLLOWING SUBSTANCES: POLYANIONS, SELECTED FROM THE GROUP CONSISTING OF HEPARIN AND DEXTRAN SULFATE, SODIUM-DODECYL-SULFATE (SOS), SODIUMPHOSPHOTUNGSTIC ACID, SODIUM OLEATE, SODIUM SALTS OF BILE
Claim 2
Independent Claim
69 words
A METHOD OF DEMONSTRATING NORMAL AND PATHOLOGICAL PLASMA-LIPOPROTEIN PATTERNS IN HUMAN BODY LIQUIDS WHICH COMPRISES ELECTROPHORETICALLY SEPARATING LIPOPROTEINS IN A CARRIER MEDIUM OR ON FOILS, UPON OCCURRENCE OF A DESIRED ELECTROPHORETICAL SEPARATION EFFECT, TREATING THE CARRIER WITH A DEVELOPING SOLUTION, COMPRISING AT LEAST ONE OF THE FOLLOWING SUBSTANCES: POLYANIONS, SELECTED FROM THE GROUP CONSISTING OF HEPARIN AND DEXTRAN SULFATE, SODIUM-DODECYL-SULFATE (SOS), SODIUMPHOSPHOTUNGSTIC ACID, SODIUM OLEATE, SODIUM SALTS OF BILE
Claim 3
Dependent Claim
13 words
A method according to claim 1, wherein the carrier medium is a gel.
Claim 4
Dependent Claim
25 words
A method according to claim 1, wherein bivalent cations, selected from the group consisting of magnesium and calcium, are additionally present in the developing solution.
Claim 5
Dependent Claim
21 words
A method according to claim 1, wherein for demonstrating LP-X-lipoprotein, a developing solution is used which comprises heparin and magnesium ions.
Claim 6
Dependent Claim
14 words
A method according to claim 4, wherein the developing solution additionally contains sodium chloride.
Claim 7
Dependent Claim
23 words
A method according to claim 1, wherein for demonstrating lipoprotein type III a developing solution is used whiCh comprises heparin and magnesium ions.
Claim 8
Dependent Claim
14 words
A method according to claim 6, wherein the developing solution additionally contains sodium chloride.
Claim 9
Dependent Claim
54 words
A method according to claim 1, wherein for selectively demonstrating lipoprotein type III and lipoproteins VLDL, LDL and HDL the carrier is successively treated with a first developing solution and a second developing solution, the first developing solution comprising heparin and magnesium ions and the second developing solution comprising dextran sulfate and calcium ions.
Claim 10
Dependent Claim
25 words
A method according to claim 1, wherein for demonstrating lipoprotein LP-X a carrier is used which already contains at least parts of the developing solution.
Claim 11
Dependent Claim
13 words
A method according to claim 9, wherein the carrier is a gel plate.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-03-24 | 1978-09-23 | 1979-03-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-03-24 | 1982-09-23 | 1983-03-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-03-24 | 1986-09-23 | 1987-03-25 | $7700.00 | Window Closed / Lapsed |