Patent US3853712A successfully refreshed from Google Patents.
US3853712A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cell culture systems
Assignee: National Research Development Corp UK
•
Filing: 1972-02-08
•
Granted: 1974-12-10
•
Expires: 1992-02-08
Abstract
Abstract The present invention comprises a method for culturing animal cells in which cells adhering to the surface of a flexible strip wound or otherwise formed by successive changes of direction into a compact cell support are contacted with culture medium. The present invention also comprises apparatus for carrying the method into effect, in which apparatus neighbouring surfaces of the support may be spaced apart to provide one or more passageways whereby in operation culture medium has access to the support to nourish cells at the surface thereof.
Inventors
- 👤 W House
- 👤 N Maroudas
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 05:56
CPC Classifications
C; C12; C12M; C12M27/00; C12M27/10; C12M27/12; C12M25/00; C12M25/02; C12M27/18; C12M27/20; C12M29/00; C12M29/06
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
52 words
CELL CULTURE APPARATUS COMPRISING A CONTAINER FOR CULTURE MEDIUM WHICH ENCLOSES A COMPACT CELL SUPPORT OF A FLEXIBLE STRIP MATERIAL FORMED INTO TURNS WHICH ARE SPACED ONE FROM ANOTHER TO PROVIDE A CONTAINUOSU PASSAGEWAY THROUGH WHICH CELLS AND MEDIUM CAN TRAVEL PROGRESSIVELY AND CONTINUOUSLY ON ROTARY MOVEMENT OF THE SUPPORT DURING INOCULATION.
Claim 2
Dependent Claim
52 words
CELL CULTURE APPARATUS COMPRISING A CONTAINER FOR CULTURE MEDIUM WHICH ENCLOSES A COMPACT CELL SUPPORT OF A FLEXIBLE STRIP MATERIAL FORMED INTO TURNS WHICH ARE SPACED ONE FROM ANOTHER TO PROVIDE A CONTAINUOSU PASSAGEWAY THROUGH WHICH CELLS AND MEDIUM CAN TRAVEL PROGRESSIVELY AND CONTINUOUSLY ON ROTARY MOVEMENT OF THE SUPPORT DURING INOCULATION.
Claim 3
Dependent Claim
43 words
Apparatus according to claim 1 which incorporates a sparge tube for aeration of the medium during cell growth said sparge tube being so disposed within the container that aeration promotes circulation of culture medium along the length of the support between the turns.
Claim 4
Dependent Claim
26 words
Apparatus according to claim 1 in which the support is a roll of flexible strip material the turns of which in cross-section approximate to a spiral.
Claim 5
Dependent Claim
28 words
Apparatus according to claim 1 in which the turns of the support are spaced apart by corrugated strips located in the region of the end of the support.
Claim 6
Dependent Claim
31 words
Apparatus according to claim 2 in which the sparge tube is axially located within a support in the form of a roll of flexible strip material having an approximately spiral cross-section.
Claim 7
Dependent Claim
24 words
Apparatus according to claim 1 in which the strip material is formed of a material selected from the group consisting of polyester and polypropylene.
Claim 8
Dependent Claim
28 words
Apparatus according to claim 1 in which the strip material is formed of polystyrene which has been treated by corona discharge so as to produce a hydrophilic surface.
Claim 9
Dependent Claim
20 words
Apparatus according to claim 1 in which the surface tension of the strip material is not less than 40 dynes/cm.
Claim 10
Dependent Claim
19 words
Apparatus according to claim 3 in which the turns of the roll are spaced 1 to 10 mm. apart.
Claim 11
Dependent Claim
30 words
Apparatus according to claim 1 in which the ratio of the surface of the support and container to the volume of the container is between 0.5 and 10 cm 1.
Claim 12
Dependent Claim
22 words
Apparatus according to claim 1 in which the container has a gas permeable wall portion through which the medium can be aerated.
Claim 13
Dependent Claim
19 words
Apparatus according to claim 11 in which the wall portion is of film 0.001 to 0.01 inches in thickness.
Claim 14
Dependent Claim
20 words
Apparatus according to claim 3 in which the outermost longitudinal edge of the roll contacts the wall of the container.
Claim 15
Dependent Claim
16 words
Apparatus according to claim 1 in which the container and cell support form a sterile unit.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-12-09 | 1978-06-10 | 1978-12-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-12-09 | 1982-06-10 | 1982-12-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-12-09 | 1986-06-10 | 1986-12-10 | $7700.00 | Window Closed / Lapsed |