Abstract A method for treating and changing the constitution of blood or other body fluid as the pancreatic islet cells effects such change normally in the blood or other body fluid passing therethrough in a live animal, in cooperation with or substitution for the animal endocrine pancreas; and a device for carrying out such treatment. The method comprises positioning on one side of a semi-permeable membrane and in contact therewith body fluid to be treated and the positioning on the opposite side of the membrane and proximate therewith once dispersed live pancreatic islet cells. The device comprises means associated with the membrane for positioning body fluid in contact with one side of the membrane, conduit means for introducing such fluid to and withdrawing it from the membrane, and once dispersed live pancreatic islet cells disposed on the other side of the membrane out of direct contact with the fluid. Additionally, the device may have a second membrane for dialyzing the fluid.
Apparatus of claim 1 wherein said membrane is permeable to molecules smaller than said cells.
Claim 2
Dependent Claim
20 words
Apparatus of claim 1 wherein the said cells are disposed in a layer having a thickness of about one cell.
Claim 3
Dependent Claim
14 words
Apparatus of claim 1 wherein the said membrane is of membrane type filter material.
Claim 4
Dependent Claim
14 words
Apparatus of claim 1 wherein the said cells are disposed in a confluent-superconfluent monolayer.
Claim 5
Dependent Claim
47 words
Apparatus of claim 1, and a second semipermeable membrane having one side juxtaposed to said first named membrane and containing said cells therebetween; and means positioning dialysate liquid onto the opposite side of said second membrane and out of direct contact with said cells and body fluid.
Claim 6
Dependent Claim
31 words
Apparatus of claim 6: wherein the said first named membrane is permeable to molecules smaller than said cells; and said second named membrane is permeable to molecules smaller than protein molecules.
Claim 7
Dependent Claim
38 words
Method of treating body fluid comprising: positioning of said fluid to be treated into contact with one side of a semipermeable membrane; and positioning of once dispersed live pancreatic islet cells proximate the opposite side of said membrane.
Claim 8
Dependent Claim
16 words
Method of claim 8 wherein the said membrane is permeable to molecules smaller than said cells.
Claim 9
Dependent Claim
48 words
Method of claim 8, and positioning of one side of a second semipermeable membrane in juxtaposition to said first membrane for containing said cells therebetween; and positioning dialysate liquid on the opposite side of said second membrane and out of direct contact with said cells and body fluid.
Claim 10
Dependent Claim
31 words
Method of claim 10: wherein the said first named membrane is permeable to molecules smaller than said cells; and said second named membrane is permeable to molecules smaller than protein molecules.
Claim 11
Dependent Claim
17 words
Method of claim 8, and dialyzing said fluid across a second semipermeable membrane with a dialysis liquid.