Fluorescence quantitative thin layer chromatographic method

Abstract

Abstract METHOD OF INCLUDING FLUORESCENT CHROMOPHORES INTO A VARIETY OF ORGANIC SUBSTRATE MOLECULES. ONE INTRODUCED INTO THE MOLECULE TO BE ANALYZED, THE FLUORESCENT CHROMOPHORE PERMITS RAPID AND ACCURATE QUANTITATIVE ANALYSIS OF THE SUBSTRATE PRECURSOR BY STANDARD FLUORESCENT SPECTROPHOTOMETRIC TECHNIQUES. THE PROCESS OF INTRODUCING THE CHROMOPHORE INVOLVES IMPREGNATING THE ABSORBENT MATERIAL WITH AMMONIUM SULFATE, SIMULTANEOUSLY DEVELOPING THE UNKNOWN WITH KNOW STANDARDS, EXPOSING THE DEVELOPED PLATE TO TERT-BUTYL HYDOCHLORITE, AND HEATING THE CHROMATOGRAM. AMINO ACIDS, FATTY ACIDS, TRIGLYCERIDES, SUGARS STEROIDS AND PROSTAGLANDINS ARE ANALYZED BY THIS TECHNIQUE IN MICROGRAM QUANTITIES.

Inventors

• 👤 B Smith

Bibliographic Details