Patent US3800035A successfully refreshed from Google Patents.
US3800035A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Production of interferon from human leukocytes in the absence of serum
Assignee: SmithKline Beecham Corp
•
Filing: 1971-12-07
•
Granted: 1974-03-26
•
Expires: 1991-12-07
Abstract
Abstract Interferon production from human leukocytes in the absence of serum is achieved by first priming the cells with interferon in the presence of serum, removing the serum, and then incubating the cells with an inducer in a serum-free medium.
Inventors
- 👤 M Goore
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:19
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; C07K14/56; Y; Y10; Y10S; Y10S435/00; Y10S435/811
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
15 words
The improvement as claimed in claim 1, in which the inducer is Newcastle disease virus.
Claim 2
Dependent Claim
14 words
The improvement as claimed in claim 1, in which the inducer is parainfluenza virus.
Claim 3
Dependent Claim
19 words
The improvement as claimed in claim 3, in which the concentration of serum in the priming step is 2-10%.
Claim 4
Dependent Claim
29 words
The improvement as claimed in claim 4, in which the nutrient medium is Eagle''s basal essential medium or minimal essential medium, and the buffer is bicarbonate, phosphate, or tricine.
Claim 5
Dependent Claim
16 words
The improvement as claimed in claim 5, in which the virus is parainfluenza virus, Sendai strain.
Claim 6
Dependent Claim
48 words
The improvement as claimed in claim 6, in which, 100 units of interferon are used in the priming step, the buffer is 0.05 M tricine at pH 7.4 or 0.01 M sodium phosphate at pH 7.2, and the amount of parainfluenza virus is 300 HA units per ml.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-03-25 | 1977-09-24 | 1978-03-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-03-25 | 1981-09-24 | 1982-03-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-03-25 | 1985-09-24 | 1986-03-26 | $7700.00 | Window Closed / Lapsed |