Patent US3766047A successfully refreshed from Google Patents.
US3766047A
Expired / Lapsed
US
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Gel for electrophoresis
Assignee: Individual
•
Filing: 1972-09-11
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Granted: 1973-10-16
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Expires: 1992-09-11
Abstract
Abstract Permeable aqueous gel of a type suitable for electrophoretic separation of proteins and other components of biological fluids such as blood serum, such gel containing as the base component of the buffer system a low molecular weight amino alcohol, also apparatus containing such gel.
Inventors
- 👤 F Elevitch
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:54
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
19 words
The medium of claim 1 containing a gelling agent capable of forming an aqueous gel consisting predominantly of water.
Claim 2
Dependent Claim
12 words
The medium of claim 2 in the form of an agarose gel.
Claim 3
Dependent Claim
21 words
The medium of claim 2 in dry form and capable of being reconstituted in gel form by the addition of water.
Claim 4
Dependent Claim
11 words
The medium of claim 1 in the form of cellulose acetate.
Claim 5
Dependent Claim
65 words
An aqueous permeable gel comprising water as a major component, a gelling agent which is compatible with electrophoresis of biological fluids to separate components thereof such as enzymes, and a buffer medium effective to maintain a pH in the gel suitable for such separation, said buffer medium including as at least a substantial part of the base component a low molecular weight amino aliphatic alcohol.
Claim 6
Dependent Claim
14 words
The aqueous permeable gel of claim 6 wherein the amino aliphatic alcohol is 2-amino-2-methyl-propan-1-01.
Claim 7
Dependent Claim
11 words
The gel of claim 6 wherein the gelling agent is agarose.
Claim 8
Dependent Claim
19 words
The gel of claim 6 wherein the amino aliphatic alcohol is 2-amino-2-methyl propan-1-01 and the gelling agent is agarose.
Claim 9
Dependent Claim
66 words
An aqueous agarose gel suitable for use as an electrophoresis medium to separate components of biological fluids by electrophoresis, said gel having agarose as a major gelling agent and being buffered by an acid-base system to a pH suitable for electrophoretic separation of proteins in biological fluids, the base component of the buffer system including as a substantial component a low molecular weight aliphatic amino alcohol.
Claim 10
Dependent Claim
10 words
The gel of claim 10 wherein the alcohol is 2-amino-2-methyl-propan-1-01.
Claim 11
Dependent Claim
19 words
The gel of claim 11 including a component which facilitates release of the gel from a hydrocarbon coated surface.
Claim 12
Dependent Claim
11 words
The gel of claim 12 wherein said component is polyvinyl alcohol.
Claim 13
Dependent Claim
12 words
The gel of claim 13 wherein the aliphatic amino alcohol is 2-amino-2-methyl-propan-1-01.
Claim 14
Dependent Claim
19 words
The gel of claim 11 including sodium barbital as a portion of the base component of the buffer system.
Claim 15
Dependent Claim
87 words
A disposable article comprising an impermeable base, an impermeable cover and a thin layer of aqueous permeable gel between said base and cover, said gel being adherent to the base and non-adherent to the cover, whereby the base may be stripped from the cover with the gel adhering thereto, said gel being buffered to maintain a pH suitable for electrophoretic separation of proteins in blood serum, the buffering medium including as at least a substantial part of the base component a low molecular weight aliphatic amino alcohol.
Claim 16
Dependent Claim
10 words
The apparatus of claim 16 wherein the alcohol is 2-amino-2-methyl-propan-1-01.
Claim 17
Dependent Claim
12 words
The article of claim 16 wherein the gel is an agarose gel.
Claim 18
Dependent Claim
15 words
The article of claim 16 wherein the cover and the base are in edge-sealing relationship.
Claim 19
Dependent Claim
21 words
The article of claim 16 wherein the base component of the buffering medium contains also a substantial proportion of sodium barbital.
References Cited (Backward Citations)
US3558459A
Cited
US3582490A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-10-15 | 1977-04-16 | 1977-10-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-10-15 | 1981-04-16 | 1981-10-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-10-15 | 1985-04-16 | 1985-10-16 | $7700.00 | Window Closed / Lapsed |