Chemically coupled enzymes

Abstract

Abstract 1283958 insolubilized enzymes CORNING GLASS WORKS 3 Sept 1969 [5 Sept 1968] 43514/69 Heading C3H An insolubilized enzyme comprises an enzyme coupled covalently to an inorganic carrier having available hydroxyl or oxide groups, said enzyme being coupled to the carrier by means of an intermediate silane coupling agent wherein the silicon portion of the molecule is attached to the carrier and the organic portion of the molecule is attached to the enzyme. The enzyme may be papain, ficin, pepsin, trypsin, chymotrypsin, bromelin, keratinase, Bacillus subtilis alkaline protease, cellulase, amylase, maltase, pectinase, chitinase, lipase, cholinesterase, lecithinase, alkaline or acid phosphatase, ribonuclease, desoxyribonuclease, arginase, asparaginase, glutaminase, urease, glucose oxidase, catalase, peroxidase, lipoxidase, cytochrome reductase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase, transmethylase or phosphopyruvic transphosphorylase. The carrier may be porous glass, colloidal silica, wollastonite, dried silica gel, bentonite, alumina, hydroxy-apatite, or nickel oxide.

Inventors

• 👤 Ralph A Messing
• 👤 Howard H Weetall

Bibliographic Details