Process and composition for the enzymatic determination of uric acid

Claim 1

A PROCESS FOR DETECTING URIC ACID IN BLOOD SERUM WHICH COMPRISES TREATING BLOOD SERUM WITH A HYDROGEN PEROXIDE GENERATING SYSTEM COMPRISING URATE OXIDASE, AN INHIBITOR FOR CATALASE SELECTED FROM THE GROUP CONSISTING OF ALKALI METAL AZIDES AND ALKALI METAL CYANIDES, A BUFFER EFFECTIVE TO MAINTAIN THE PH OF THE GENERATING SYSTEM IN A RANGE OF ABOUT FROM PH 8.5 TO PH 10 IN WHICH SAID URATE OXIDASE IS EFFECTIVE TO CONVERT URIC ACID TO ALLANTOIN WITH THE CONCOMITANT PRODUCTION OF HYDROGEN PEROXIDE FOR A PERIOD SUFFICIENT TO CONVERT THE URIC ACID PRESENT IN THE BLOOD SERUM T ALLANTOIN WITH THE CONCOMITANT PRODUCTION OF HYDROGEN PEROXIDE BEING QUANTITATIVE AND THEREAFTER CONTACTING THE RESULTING MIXTURE WITH A HYDROGEN PEROXIDE DETECTING SYSTEM COMPRISING A CHROMOGEN, A SUBSTANCE HAVING PEROXIDATIVE ACTIVITY EFFECTIVE TO CATALYZE THE OXIDATION OF SAID CHROMOGEN BY HYDROGEN PEROXIDE WITH THE PLRODUCTION OF A COLOR CHANGE, AND A BUFFER EFFECTIVE TO MAINTAIN THE PH OF THE RESULTING MIXTURE IN A RANGE OF ABOUT FROM PH 4.5 TO PH 5.5 AND THEREAFTER OBSERVING THE COLOR DEVELOPED AND CORRELATING SAME TO THE QUANTITY OF URIC ACID PRESENT IN THE BLOOD.