Patent EP0638805A2 successfully refreshed from Google Patents.
EP0638805A2
Expired / Lapsed
EP
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Analyte detection device and process
Assignee: Bayer Corp
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Filing: 1994-07-25
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Granted: —
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Expires: 2014-07-25
Abstract
Abstract The present invention provides a device and a process for detecting an analyte in a biological fluid. The device comprises a separating matrix for separating analyte from the fluid and means for detecting the analyte. where the separating matrix contains HEPES buffer, preferably in an amount between 70 and 150 millimolar. The process comprises applying the sample to the device having a separating matrix and then detecting analyte in the sample using the detection means. The presence of HEPES in the separation layer shortened the detection time.
Inventors
- 👤 Amy H. Chu
- 👤 Michael J. Wilcox
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 10:09
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/54; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333; Y10T436/144444; Y10T436/25; Y10T436/25375
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
58 words
A device for detecting an analyte in a biological fluid,said device comprising: a) a separation matrix containing HEPES; and b) means for detecting said analyte, which detection means is vertically adjacent to the separation matrix and substantially coincident with that matrix such that said analyte can move from the separation matrix to the means for detecting said analyte.
Claim 2
Dependent Claim
38 words
The device according to claim 1 wherein said detection means is a membrane containing one or more chemical reagents that interact with the analyte to generate a detectable signal indicative of said analyte presence in said biological fluid.
Claim 3
Dependent Claim
20 words
The device according to claim 2 wherein said chemical reagents comprise ATP, NAD, hexokinase, glueose-6-phosphate dehydrogenase, diaphorase and an indicator.
Claim 4
Dependent Claim
14 words
The device according to claim 1 wherein said separation matrix is a membrane filter.
Claim 5
Dependent Claim
15 words
The device according to claim 4 wherein said membrane filter is a glass fiber filter.
Claim 6
Dependent Claim
15 words
The device according to claim 1 wherein said separation matrix contains a surface active agent.
Claim 7
Dependent Claim
62 words
The device according to claim 1 further comprising: a) a base portion having a transparent window, which base portion is situated such that said detection means is vertically adjacent to said base portion and at least partially coincident with said transparent window: b) a cover portion having an aperture, which cover portion is vertically adjacent to and coincident with said separation matrix.
Claim 8
Dependent Claim
22 words
The device of claim 1 in which the amount of HEPES present in the separation matrix is between 70 and 150 millimolar.
Claim 9
Independent Claim
56 words
A device for assaying blood glucose comprising: a) a synthetic membrane containing ATP, NAD, hexokinase, glucose-6-phosphate dehydrogenase, diaphorase and an indicator; and b) a glass fiber filter vertically adjacent to said synthetic membrane and substantially coincident with said synthetic membrane in which the glass fiber filter contains HEPES in an amount between 70 to 150 millimolar.
Claim 10
Dependent Claim
92 words
A process of detecting an analyte in a biological fluid, said process comprising the steps of: a) providing a detection device comprising: i) a separation matrix containing HEPES; and ii) means for detecting said analyte, which detection means is vertically adjacent to the separation matrix and substantially coincident with that matrix such that said analyte can move from the separation matrix to the means for detecting said analyte; b) applying a sample of said biological fluid to said separation matrix: and c) determining a reaction between said analyte and said detection means.
References Cited (Backward Citations)
No citation records indexed.
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