Patent US1725454A successfully refreshed from Google Patents.
EP0597510A1
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EP
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Biologically active reagent prepared from aldehyde-containing polymer, test kit, analytical element and methods of use
Assignee: Ortho Clinical Diagnostics Inc
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Filing: 1993-09-25
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Granted: —
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Expires: 2013-09-25
Abstract
Abstract A biologically active reagent is prepared by reacting a biologically active substance covalently to a polymeric particle having pendant aldehyde groups. Such groups are provided by a polymerizable monomer represented by the structure: wherein Ar is arylene, R and R¹ are independently hydrogen, halo or lower alkyl, R² and R³ are independently alkylene of 1 to 4 carbon atoms in the chain, R⁴ is arylene, m is 0 or 1, and n is an integer of 1 to 4. These reagents can be used in a variety of specific binding and affinity purification methods, and in both solution assays and dry analytical elements.
Inventors
- 👤 Richard Calvin C/O Eastman Kodak Company Sutton
- 👤 Ignazio S. C/O Eastman Kodak Company Ponticello
- 👤 Susan Jean C/O Eastman Kodak Company Danielson
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 02:32
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6834; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/54366; G01N33/54386; G01N33/544; G01N33/545
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
155 words
A biologically active reagent comprising: (I) a water-insoluble particle which is non-swellable in water and (II) a biologically active substance covalently attached to the particle, the reagent characterized wherein the particle outer surface is composed of a polymer derived from an ethylenically unsaturated polymerizable monomer having a pendant aldehyde group and represented by the structure: wherein Ar is substituted or unsubstituted arylene of 6 to 14 carbon atoms in the aromatic nucleus, R and R¹ are independently hydrogen, halo or substituted or unsubstituted lower alkyl of 1 to 4 carbon atoms, R² and R³ are independently substituted or unsubstituted alkylene of 1 to 4 carbon atoms in the chain, R⁴ is substituted or unsubstituted arylene of 6 to 14 carbon atoms in the aromatic nucleus, m is 0 or 1, and n is an integer of 1 to 4, and the biologically active substance is covalently attached to the particle through the pendant aldehyde group.
Claim 2
Dependent Claim
32 words
The reagent as claimed in claim 1 wherein Ar is phenylene, R is hydrogen, R¹ is hydrogen or methyl, R² is methylene, R⁴ is phenylene, m is 0 and n is 1.
Claim 3
Dependent Claim
57 words
The reagent as claimed in either of claims 1 and 2 wherein the monomer is selected from the group consisting of o -, p - or m -formylphenyl vinylbenzyl ether, o -, p - or m -(2-formylethylphenyl vinylbenzyl ether, 2-, 3- or 4-formylnaphthyl vinylbenzyl ether, (3- or 4-formyl-2-methylphenyl) vinyl benzyl ether, formylbiphenylyl vinylbenzyl ether and 4-{4-[4-(4-formylphenoxymethyl)phenoxymethyl]phenoxymethyl} styrene.
Claim 4
Dependent Claim
167 words
The reagent as claimed in any of claims 1 to 3 wherein the polymer has recurring units derived from: (a) 1 to 100 weight percent of one or more ethylenically unsaturated polymerizable monomers represented by the structure: wherein Ar is substituted or unsubstituted arylene of 6 to 14 carbon atoms in the aromatic nucleus, R and R¹ are independently hydrogen, halo or substituted or unsubstituted lower alkyl of 1 to 4 carbon atoms, R² and R³ are independently substituted or unsubstituted alkylene of 1 to 4 carbon atoms in the chain, R⁴ is substituted or unsubstituted arylene of 6 to 14 carbon atoms in the aromatic nucleus, m is 0 or 1, and n is an integer of 1 to 4, (b) 0 to 99 weight percent of one or more hydrophobic ethylenically unsaturated polymerizable monomers other than those defined in component (a), and (c) 0 to 20 weight percent of one or more ethylenically unsaturated polymerizable monomers other than those defined in components (a) and (b).
Claim 5
Dependent Claim
22 words
The reagent as claimed in any of claims 1 to 4 wherein the biologically active substance is an antibody or nucleic acid.
Claim 6
Dependent Claim
37 words
An analytical element comprising a fluid-permeable substrate having one or more reaction zones therein, and containing in at least one of the reaction zones, a biologically active reagent as claimed in any of claims 1 to 5.
Claim 7
Dependent Claim
63 words
An analytical element comprising a nonporous support, having disposed thereon, in order and in fluid contact, a reagent layer containing one or more reagents for providing a detectable signal in response to a ligand of interest, and a porous spreading layer, the element further containing, in one or more layers, a biologically active reagent as claimed in any of claims 1 to 5.
Claim 8
Dependent Claim
30 words
The element as claimed in claim 7 further comprising a detectably labeled analog of the ligand which is located in a layer other than that containing the biologically active reagent.
Claim 9
Independent Claim
79 words
A method for the determination of a specific binding ligand comprising: A. forming a water-insoluble specific binding complex of a specific binding ligand of interest, or a receptor therefor, with a reagent as claimed in any of claims 1 to 5 wherein the biologically active substance of the reagent is specifically reactive with either the ligand or a receptor therefor, and B. detecting the presence of the complex as an indication of the presence or amount of the ligand.
Claim 10
Dependent Claim
29 words
The method as claimed in claim 9 wherein the ligand is an antigenic material and the receptor is an antibody specific to the antigenic material or a component thereof.
Claim 11
Dependent Claim
27 words
The method as claimed in claim 9 wherein the ligand is a single stranded nucleic acid and the receptor is an oligonucleotide complementary to the nucleic acid.
Claim 12
Dependent Claim
88 words
An analytical separation method comprising: A. passing a specimen containing a mixture of biologically active substances over an affinity chromatography reagent as claimed in any of claims 1 to 5 wherein the reagent comprises a specific binding substance specific to one or more predetermined biologically active substances in the specimen mixture of biologically active substances, to form a complex of the reagent with the predetermined biologically active substances, and B. collecting either the one or more complexed predetermined substances or one or more substances remaining in the eluent.
Claim 13
Independent Claim
75 words
A method for the detection of a nucleic acid comprising: A. forming a water-insoluble hybridization product between a nucleic acid of interest and a reagent as claimed in any of claims 1 to 5 wherein the reagent comprises an oligonucleotide covalently attached to the particle through the pendant aldehyde group, the oligonucleotide being complementary to the nucleic acid of interest, and B. detecting the hybridization product as a determination of the nucleic acid of interest.
Claim 14
Dependent Claim
39 words
A kit comprising, separately packaged: one or more water-soluble reagents useful for the determination of the specific binding species, the kit characterized wherein it also includes a biologically active reagent as claimed in any of claims 1 to 5.
References Cited (Backward Citations)
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