EP0361957A1
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Tissue equivalents and methods of preparation thereof
Assignee: Organogenesis Inc
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Filing: 1989-09-29
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Granted: —
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Expires: 2009-09-29
Abstract
Abstract The present invention provides tissue equivalents which have cell types differentiated from progenitor cells without exogenous chemical induction, and methods of making and using such tissue equivalents. The present invention also provides methods for differentiating progenitor cells without exogenous chemical induction.
Inventors
- 👤 Eugene Bell
- 👤 David T. Kagan
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-22 22:32
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0652; C12N5/0653; A; A61; A61L; A61L27/00; A61L27/14; A61L27/22; A61L27/24; A61L27/50; A61L27/60; A61F; A61F2310/00; A61F2310/00005; A61F2310/00365; A61K; A61K35/00; A61K35/12; C12N2501/00; C12N2501/01; C12N2501/30; C12N2501/38; C12N2501/39; C12N2502/00; C12N2502/13; C12N2502/1323; C12N2533/00; C12N2533/50; C12N2533/54
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
87 words
A method of producing a tissue equivalent comprising: a. constituting a tissue precursor mixture, the mixture comprising a collagen solution, at least one contractile agent and at least one type of progenitor cell, under conditions to form a gel having the contractile agent and progenitor cells dispersed within the gel; and then b. maintaining the gel prepared in step (a) under conditions which permit (i) the contraction of the gel to form a tissue equivalent and (ii) the differentiation without exogenous chemical induction of the progenitor cells.
Claim 2
Independent Claim
87 words
A method of producing a tissue equivalent comprising: a. constituting a tissue precursor mixture, the mixture comprising a collagen solution, at least one contractile agent and at least one type of progenitor cell, under conditions to form a gel having the contractile agent and progenitor cells dispersed within the gel; and then b. maintaining the gel prepared in step (a) under conditions which permit (i) the contraction of the gel to form a tissue equivalent and (ii) the differentiation without exogenous chemical induction of the progenitor cells.
Claim 3
Dependent Claim
11 words
A tissue equivalent formed according to the method of claim 1.
Claim 4
Dependent Claim
30 words
The method of claim 1, wherein the progenitor cells are preadipocytes, undifferentiated embryonic cells, undifferentiated fetal cells, tissue cells dedifferentiated during cell culture, or organ cells dedifferentiated during cell culture.
Claim 5
Dependent Claim
14 words
The method of claim 1, wherein the tissue precursor mixture further comprises non-inducible fibroblasts.
Claim 6
Independent Claim
59 words
A method of augmenting tissue in a living host comprising: a. administering at the site to be augmented a tissue precursor mixture, the mixture comprising a collagen solution, at least one contractile agent and at least one type of progenitor cell, under conditions to form a gel having the contractile agent and progenitor cells dispersed within the gel mixture.
Claim 7
Independent Claim
59 words
A method of augmenting tissue in a living host comprising: a. administering at the site to be augmented a tissue precursor mixture, the mixture comprising a collagen solution, at least one contractile agent and at least one type of progenitor cell, under conditions to form a gel having the contractile agent and progenitor cells dispersed within the gel mixture.
Claim 8
Independent Claim
83 words
A method of producing a tissue equivalent comprising: a. constituting a tissue precursor mixture, the mixture comprising a collagen solution, at least one contractile agent and preadipocytes, under conditions to form a gel having the contractile agent and preadipocytes dispersed within the gel; and then b. maintaining the gel mixture prepared in step (a) under conditions which permit (i) the contraction of the gel mixture to form a tissue equivalent, and (ii) the differentiation without exogenous chemical induction of the preadipocytes into adipocytes.
Claim 9
Independent Claim
83 words
A method of producing a tissue equivalent comprising: a. constituting a tissue precursor mixture, the mixture comprising a collagen solution, at least one contractile agent and preadipocytes, under conditions to form a gel having the contractile agent and preadipocytes dispersed within the gel; and then b. maintaining the gel mixture prepared in step (a) under conditions which permit (i) the contraction of the gel mixture to form a tissue equivalent, and (ii) the differentiation without exogenous chemical induction of the preadipocytes into adipocytes.
Claim 10
Dependent Claim
11 words
A tissue equivalent formed according to the method of claim 6.
Claim 11
Independent Claim
53 words
A method of augmenting tissue in a living host comprising: a. administering at the site to be augmented a tissue precursor mixture, the mixture comprising collagen, at least one contractile agent and preadipocytes, under conditions to form at the site a gel having the contractile agent and preadipocytes dispersed within the gel mixture.
Claim 12
Independent Claim
53 words
A method of augmenting tissue in a living host comprising: a. administering at the site to be augmented a tissue precursor mixture, the mixture comprising collagen, at least one contractile agent and preadipocytes, under conditions to form at the site a gel having the contractile agent and preadipocytes dispersed within the gel mixture.
Claim 13
Dependent Claim
29 words
A method of augmenting tissue in a living host comprising: a. implanting a tissue equivalent formed according to the method of claim 2 at the site to be augmented.
Claim 14
Dependent Claim
29 words
A method of augmenting tissue in a living host comprising: a. implanting a tissue equivalent formed according to the method of claim 6 at the site to be augmented.
Claim 15
Independent Claim
81 words
A method of differentiating progenitor cells comprising: a. constituting a mixture, the mixture comprising a collagen solution, at least one contractile agent and at least one type of progenitor cell, under conditions to form a gel having the contractile agent and progenitor cells dispersed within the gel mixture; and then b. maintaining the gel mixture prepared in step (a) under conditions which permit (i) the contraction of the gel mixture, and (ii) differentiation, without exogenous chemical induction, of the progenitor cells.
Claim 16
Independent Claim
81 words
A method of differentiating progenitor cells comprising: a. constituting a mixture, the mixture comprising a collagen solution, at least one contractile agent and at least one type of progenitor cell, under conditions to form a gel having the contractile agent and progenitor cells dispersed within the gel mixture; and then b. maintaining the gel mixture prepared in step (a) under conditions which permit (i) the contraction of the gel mixture, and (ii) differentiation, without exogenous chemical induction, of the progenitor cells.
Claim 17
Dependent Claim
30 words
The method of claim 11, wherein the progenitor cells are preadipocytes, undifferentiated embryonic cells, undifferentiated fetal cells, tissue cells dedifferentiated during cell culture, or organ cells dedifferentiated during cell culture.
References Cited (Backward Citations)
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