Method for seeding polymeric surfaces with human endothelial cells

Abstract

Abstract The method comprises modifying the surface of the polymer (1) with suitable functional groups (2). The functional groups permit covalent bonding of a biopolymer (3) to the support polymer. The polymer surface prepared in this way with components intrinsic to the cells (biopolymer) is the basis for the colonisation by human endothelial cells (4). An extracellular matrix (5) is deposited between the biopolymer and the endothelial cells by the latter. This extracellular matrix is likewise bound to the natural adhesion sites of the biopolymer (6) and thus strengthens the natural basis of the endothelial cells. The particular feature of this method is the permanent binding of a biopolymer to the underlying support polymer with the beneficial effect that functioning endothelial cells are able to colonise the latter as monolayer. <IMAGE>

Inventors

• 👤 Christian Dr. Med. Mittermayer
• 👤 Doris Dr. Rer. Nat. Klee
• 👤 Horst Dr.-Ing. Richter

Bibliographic Details