Patent EP0123443A2 successfully refreshed from Google Patents.
EP0123443A2
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EP
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Method of analyzing whole blood sample
Assignee: Fujifilm Holdings Corp
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Filing: 1984-03-26
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Granted: —
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Expires: 2004-03-26
Abstract
Abstract A method of quantitative analysis of an analyte contained in a whole blood sample using a multilayer analytical element comprising a transparent support, at least one reagent layer and a porous spreading layer capable of supplying a liquid sample spotted on the surface of said spreading layer to said reagent layer keeping a substantially constant volume per unit area, the method comprising spotting the whole blood sample on the porous spreading layer: which is characterized in that the whole blood sample is a blood sample prepared by dilution of a whole blood with an aqueous diluent in a specified volume ratio.
Inventors
- 👤 Yuzo Iwata
- 👤 Masao Kitajima
- 👤 Asaji Kondo
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 11:50
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
120 words
1 . A method of quantitatively analyzing an analyte contained in a whole blood sample by the use of a multilayer analytical element having at least one reagent layer provided on one side of a light-transmissive water-impermeable support and a porous spreading layer forming the outermost layer of said element and being able to supply a liquid sample applied on the surface of said spreading layer to said reagent layer keeping a substantially constant volume per unit area, the method comprising applying the whole blood sample on the porous spreadfng layer: which is characterized in that the whole blood sample is a blood sample prepared by dilution of a whole blood with an aqueous diluent in a specified volume ratio.
Claim 2
Dependent Claim
120 words
1 . A method of quantitatively analyzing an analyte contained in a whole blood sample by the use of a multilayer analytical element having at least one reagent layer provided on one side of a light-transmissive water-impermeable support and a porous spreading layer forming the outermost layer of said element and being able to supply a liquid sample applied on the surface of said spreading layer to said reagent layer keeping a substantially constant volume per unit area, the method comprising applying the whole blood sample on the porous spreadfng layer: which is characterized in that the whole blood sample is a blood sample prepared by dilution of a whole blood with an aqueous diluent in a specified volume ratio.
Claim 3
Dependent Claim
35 words
The method of analysis as claimed in claim 1, wherein said specified volume ratio is within the range of from 0.1 to 10 volumes of the aqueous diluent per one volume of the whole blood.
Claim 4
Dependent Claim
35 words
The method of analysis as claimed in claim 1, wherein said specified volume ratio is within the range of from 0.5 to 3 volumes of the aqueous diluent per one volume of the whole blood.
Claim 5
Dependent Claim
19 words
The method of analysis as claimed in claim 1, wherein said aqueous diluent does not hemolyze the whole blood.
Claim 6
Dependent Claim
23 words
The method of analysis as claimed in claim 4, wherein said aqueous diluent is a substantially isotonic ) solution of the whole blood.
Claim 7
Dependent Claim
19 words
The method of analysis as claimed in claim 5, wherein said substantially isotonic solution is a physiological saline solution.
Claim 8
Dependent Claim
32 words
The method of analysis as claimed in claim 5, wherein said substantially isotonic solution is an aqueous solution of a hydrophilic water-soluble or water- dispersable organic substance in a physiological saline solution.
Claim 9
Dependent Claim
28 words
The method of analysis as claimed in claim 5, wherein said substantially isotonic solution is an aqueous solution of a water-miscible organic liquid in a physiological saline solution.
Claim 10
Dependent Claim
29 words
The method of analysis as claimed in claim 6, wherein said organic liquid is at least one compound selected from the group consisting of methanol, ethanol and benzyl alcohol.
Claim 11
Dependent Claim
27 words
The method of analysis as claimed in claim 1, wherein said dilution of the whole blood is performed by adding the aqueous diluent to the whole blood.
Claim 12
Dependent Claim
27 words
The method of analysis as claimed in claim 1, wherein said dilution of the whole blood is performed by adding the whole blood to the aqueous diluent.
Claim 13
Dependent Claim
35 words
The method of analysis as claimed in claim 1, wherein said dilution of the whole blood is performed by introducing the whole blood and the aqueous diluent in a vessel at a substantially same time.
Claim 14
Dependent Claim
103 words
The method of analysis as claimed in claim 1, wherein said dilution of the whole blood is performed by the use of a spotting means capable of producing a liquid drop at the pointed end through pushing out the liquid sample contained therein, in which the diluting process comprises successively drawing up a specified volume of the aqueous diluent and a specified volume of the whole blood into the spotting means and the applying process is performed by pushing out both liquids through the pointed end of the spotting means to spot the liquids on the spreading layer of the multilayer analytical element.
Claim 15
Dependent Claim
16 words
The method of analysis as claimed in claim 13, wherein said spotting means is a micropipette.
References Cited (Backward Citations)
No citation records indexed.
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