Patent EP0046345A2 successfully refreshed from Google Patents.
EP0046345A2
Expired / Lapsed
EP
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Controlled hydrodynamic flow in flow cytometry systems
Assignee: Ortho Clinical Diagnostics Inc
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Filing: 1981-07-28
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Granted: —
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Expires: 2001-07-28
Abstract
Abstract Sheath fluid and sample fluid are both provided at respective constant mass flow rates through the flow cell of a flow cytometry system. Sample fluid is provided by means of a precision positive displacement pump utilizing a motor driven screw and syringe system. Sheath fluid is provided either using a similar positive displacement system, or utilizing a pressurized source and a differential pressure regulator in the sheath flow line.
Inventors
- 👤 J. Garland O'connell
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 20:07
CPC Classifications
G; G01; G01N; G01N15/00; G01N15/10; G01N15/14; G01N15/1404; G01N35/00; G01N35/10; G01N35/1095
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
114 words
In a flow cytometry system having a flow channel carrying a hydrodynamically focused sample fluid stream enclosed within a sheath fluid stream, apparatus for supplying said streams under predetermined precise flow conditions comprising: a) a supply of sheath fluid; b) means for coupling sheath fluid to said flow channel at a constant mass flow rate; c) positive displacement precision pump means including ,i) syringe means comprising a plunger and a matable cylinder, and (ii) means for displacing said plunger at predetermined rates within said cylinder; d) means for coupling sample fluid into said cylinder; and e) · means for delivering sample fluid from said cylinder to said flow channel under pressure from said plunger.
Claim 2
Dependent Claim
114 words
In a flow cytometry system having a flow channel carrying a hydrodynamically focused sample fluid stream enclosed within a sheath fluid stream, apparatus for supplying said streams under predetermined precise flow conditions comprising: a) a supply of sheath fluid; b) means for coupling sheath fluid to said flow channel at a constant mass flow rate; c) positive displacement precision pump means including ,i) syringe means comprising a plunger and a matable cylinder, and (ii) means for displacing said plunger at predetermined rates within said cylinder; d) means for coupling sample fluid into said cylinder; and e) · means for delivering sample fluid from said cylinder to said flow channel under pressure from said plunger.
Claim 3
Dependent Claim
39 words
Apparatus as described in claim 1 wherein said means for displacing comprises: a) a motor driving a lead screw, and b) nut means, threadedly matable with and translated by said lead screw, to displace said plunger within said chamber.
Claim 4
Dependent Claim
27 words
Apparatus as described in claim 2 wherein said means for delivering sample fluid includes flow oscillation damper means connected between said syringe means and said flow channel.
Claim 5
Dependent Claim
31 words
Apparatus as described in claim 1 and further including means for pressurizing said supply of sheath fluid, and wherein said means for coupling sheath fluid comprises sheath flow pressure regulation means.
Claim 6
Dependent Claim
56 words
Apparatus as described in claim 4 wherein said pressure regulation means includes differential pressure regulation means for sensing fluid pressure between two given points intermediate said sheath fluid supply and said flow channel, and flow constriction means, connected between said points, controlled by said pressure regulation means, for maintaining a constant pressure drop between said points.
Claim 7
Dependent Claim
74 words
Apparatus as described in claim 1 wherein said means for coupling sheath fluid comprises: a) positive displacement precision pump means including (i) second syringe means comprising a second plunger and matable cylinder, and (ii) means for displacing said second plunger at predetermined rates within its matable cylinder; b) means for coupling sheath fluid into said second syringe means; and c) means for delivering sheath fluid from said second syringe means to said flow channel.
References Cited (Backward Citations)
No citation records indexed.
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