EP0038960A2 — Diagnostic reagents for immunological tests and a…

Claim 1

Independent Claim 59 words

A diagnostic reagent for immunological testing comprising immobilizing immunochemicals on a particulate carrier, characterized in that fine particles having an average diameter within the range of about 0.03 - 10 µm and comprising a cross-linked polyner having a repeating unit represented by the general formula wherein R denotes hydrogen or a methyl group are used as said particulate carrier.

Claim 2

Dependent Claim 59 words

A diagnostic reagent for immunological testing comprising immobilizing immunochemicals on a particulate carrier, characterized in that fine particles having an average diameter within the range of about 0.03 - 10 µm and comprising a cross-linked polyner having a repeating unit represented by the general formula wherein R denotes hydrogen or a methyl group are used as said particulate carrier.

Claim 3

Dependent Claim 69 words

A diagnostic reagent for immunological testing according to claim 1, characterized in that as said particulate carrier, said fine particles are comprised of a cross-linked polymer containing not less than about 50 mol % of the repeating unit represented by the general formula [I]. and less than about 50 mol % of a copolymerizable monomer unit selected from the group consisting of 2-hydroxyethyl acrylate and 2-hydroxyethyl methacrylate, are used.

Claim 4

Dependent Claim 44 words

A diagnostic reagent for immunological testing according to claim 1, characterized in that said immunochemicals are selected from the group consisting of Treponema pallidum antigen, human chorionic gonadtropin, anti-human chorionic gonadtropin antibody, anti-human IgG antibody, anti-human IgM antibody, aggregated human IgG and protein A.

Claim 5

Dependent Claim 109 words

A process for preparing diagnostic reagents for immunological tests which comprises polymerizing a mixture of addition polymerizable monomers containingnot less than about 50 mol % of glycidyl acrylate and glycidyl methacrylate in a medium in which said mixture of monomers is soluble but in which the polymer produced is not soluble, treating the fine particulate polymer precipitated with a hydrolyzing agent and immobilizing immunochemicals by covalent bonding on fine particles having an average diameter within the range of about 0.03 - 10 pm and consisting of the so obtained cross-linked polymer having a repeating unit represented by the general formula [I] wherein R denotes hydrogen or a methyl group.

Claim 6

Dependent Claim 109 words

A process for preparing diagnostic reagents for immunological tests which comprises polymerizing a mixture of addition polymerizable monomers containingnot less than about 50 mol % of glycidyl acrylate and glycidyl methacrylate in a medium in which said mixture of monomers is soluble but in which the polymer produced is not soluble, treating the fine particulate polymer precipitated with a hydrolyzing agent and immobilizing immunochemicals by covalent bonding on fine particles having an average diameter within the range of about 0.03 - 10 pm and consisting of the so obtained cross-linked polymer having a repeating unit represented by the general formula [I] wherein R denotes hydrogen or a methyl group.

Claim 7

Dependent Claim 109 words

A process for preparing diagnostic reagents for immunologicai tests according to claim 4, characterized in that either before or after treating said fine particulate polymer with a hydrolyzing agent, said fine particulate polymer is treated with an aminating agent selected from the group consisting of ammonia, hydrazine or an organic amine having not less than 2 amino groups within the molecule, to thereby introduce an amino group into said fine particulate polymer and immunochemicals having an amino group are immobilized on said fine particulate cross-linked polymer containing an amino group using a compound having 2 or more functional groups bondable to amino groups within the molecule as a binder.

Claim 8

Dependent Claim 99 words

A process for preparing diagnostic reagents for immunological tests according to claim 4, characterized in that said mixture of addition polymerizable monomers contain up to about 30 mol % of an addition polymerizable monomer having a carboxyl group to thereby introduce a carboxyl group to said fine particulate cross-lined polymer having said repeating unit represented by the general formula [I], amide bond is caused to be produced from the amino group of said immunochemicals having an amino group and the carboxyl group of said fine particulate cross-linked polymer to thereby immobilize said immunochemicals on said fine particulate cross-linked polymer.

Claim 9

Dependent Claim 37 words

A composition of matter useful in diagnostic immunological testing as an immobilizing carrier for immunochemicals comprising: a cross-linked polymer having a repeating unit represented by the general formula [I] wherein R denotes hydrogen or a methyl group.

Claim 10

Dependent Claim 37 words

A composition of matter useful in diagnostic immunological testing as an immobilizing carrier for immunochemicals comprising: a cross-linked polymer having a repeating unit represented by the general formula [I] wherein R denotes hydrogen or a methyl group.

Claim 11

Dependent Claim 24 words

The composition of claim 7 wherein said composition is particulate and of particle size in the range of from about 0.03 to about 10µm.

Claim 12

Dependent Claim 49 words

The composition of claim 7 wherein said composition is comprised of about at least 50 mol % of the repeating unit represented by the general formula [I] and less than about 50 mol % of a copolymerizable monomer unit selected from the group consisting of 2-hydroxyethylacrylate and 2-hydroxyethyl methacrylate.

Claim 13

Dependent Claim 69 words

A reagent useful in diagnostic immunological testing comprising (1) an immobilizing carrier including a cross-linked polymer having a repeating unit represented by the general formula wherein R denotes hydrogen or a methyl group, and (2) an immunochemical, bonded to said carrier, selected from the group consisting of Treponema pallidum antigen, human chorionic gonadtropin, anti-human chorionic gonadtropin antibody, anti-human IgG antibody, anti-human IgM antibody, aggregated human IgG and protein A.

Claim 14

Dependent Claim 69 words

A reagent useful in diagnostic immunological testing comprising (1) an immobilizing carrier including a cross-linked polymer having a repeating unit represented by the general formula wherein R denotes hydrogen or a methyl group, and (2) an immunochemical, bonded to said carrier, selected from the group consisting of Treponema pallidum antigen, human chorionic gonadtropin, anti-human chorionic gonadtropin antibody, anti-human IgG antibody, anti-human IgM antibody, aggregated human IgG and protein A.

Claim 15

Dependent Claim 96 words

A process for producing a composition of matter useful in diagnostic immunological testing as an immobilizing carrier for immunochemicals comprising (1) polymerizing a mixture of addition polymerizable monomers containing more than 50 mol % of glycidyl acrylate and glycidyl methacrylate in a medium in which said mixture of monomers is soluble, but in which the polymer produced is not soluble, to produce a polymer, and (2) treating said polymer with a hydrolyzing agent to produce a cross-linked polymer having a repeating unit represented by the general formula [I] wherein R denotes hydrogen or a methyl group.

Claim 16

Dependent Claim 96 words

A process for producing a composition of matter useful in diagnostic immunological testing as an immobilizing carrier for immunochemicals comprising (1) polymerizing a mixture of addition polymerizable monomers containing more than 50 mol % of glycidyl acrylate and glycidyl methacrylate in a medium in which said mixture of monomers is soluble, but in which the polymer produced is not soluble, to produce a polymer, and (2) treating said polymer with a hydrolyzing agent to produce a cross-linked polymer having a repeating unit represented by the general formula [I] wherein R denotes hydrogen or a methyl group.

Claim 17

Dependent Claim 26 words

The process of claim 11 wherein said polymer is particulate with particles having an average diameter within the range of about 0.03 to about 10 µm.

Claim 18

Dependent Claim 46 words

The process of claim 11 further comprising the step of treating said polymer with an aminating agent selected from the group consisting of ammonia, hydrazine or an organic amine having 2 or more amino groups within the molecule, to add an amino group onto said polymer.

Claim 19

Dependent Claim 39 words

The process of claim 13 further comprising immobilizing immunochemicals having an amino group on said polymer containing an amino group using a compound having 2 or more functional groups, bondable to amino groups within the molecule, as a binder.

Claim 20

Dependent Claim 36 words

The process of claim 11 further comprising introducing a carboxyl group to said polymer by including in said mixture of addition polymerizable monomers up to about 30 mol % of a monomer having a carboxyl group.

Claim 21

Dependent Claim 32 words

The process of claim 15 further comprising producing an amide bond from said carboxyl group on said polymer to an amino group on said immunochemical to immobilize said immunochemical on said polymer.

Diagnostic reagents for immunological tests and a process for preparing the same

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