Enzymatic determination of nitrogen- containing compounds and enzymes reactive therewith
Abstract
Abstract A PROCESS FOR MEASURING THE AMOUNT OF A NITROGENCONTAINING COMPOUND IN A SAMPLE OR THE AMOUNT OF AN ENZYME IN A SAMPLE, THE ENZYME BEING REACTIVE WITH A NITROGEN-CONTAINING COMPOUND, WHICH INVOLVES (1) MIXING THE SAMPLE WITH A COMPOUND THAT IS ENZYMATICALLY REACTIVE WITH AMMONIA; (2) ADDING TO THE NITROGENCONTAINING COMPOUND IN THE SAMPLE AN AMOUNT OF THE ENZYME OR ADDING TO THE ENZYME A NON-RATE LIMITING AMOUNT OF A NITROGEN-CONTAINING COMPOUND TO RELEASE AMMONIA, IN EITHER CASE, AT A MEASURABLE RATE; AND (3) MEASURING THE RATE OF REACTION OF AMMONIA WITH THE COMPOUND THAT IS REACTIVE WITH AMMONIA, THIS RATE OF REACTION BEING PROPORTIONAL TO THE CONCENTRATION OF THE NITROGEN-CONTAINING COMPOUND OR THE CONCENTRATION OF THE ENZYME, AS THE CASE MAY BE, IN THE ORIGINAL SAMPLE.
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