US6352555B1
Expired / Lapsed
US
⏱️ 0.0 years remaining
Methods for implanting cells
Assignee: The Brigham and Women's Hospital Inc
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Filing: 1999-07-08
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Granted: 2002-03-05
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Expires: 2019-07-08
Abstract
Abstract Disclosed herein is a method for implanting cells onto a prosthesis, including the steps of: (a) providing a prosthesis including a porous tube, where at least 25% of the pores on the inner surface of the tube have diameters of more than about 40 μm, at least 25% of the pores on the outer surface of the tube have diameters of less than about 30 μm, and the tube includes a substantially continuous layer of a biocompatible material; (b) contacting the prosthesis with a suspension of cells; and (c) providing a pressure differential between the inner surface and the outer surface, whereby the cells are retained in the pores of the inner surface. Also disclosed herein are methods for culturing cells for implantation.
Inventors
- 👤 Victor J. Dzau
- 👤 Richard E. Pratt
- 👤 Michael J. Mann
- 👤 Afshin Ehsan
- 👤 Daniel P. Griese
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 00:40
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/36; A61L27/38; A61L27/3804; A61L27/3808; A61F; A61F2/00; A61F2/02; A61F2/04; A61F2/06; A61F2/062; A61L27/3895; A61L27/50; A61L27/507; A61L27/56; A61P; A61P9/00; C; C12; C12N; C12N5/00; C12N5/0068; C12N5/06; C12N5/0602; C12N5/069; A61K; A61K35/00; A61K35/12; C12N2501/00; C12N2501/10; C12N2501/105; C12N2501/115; C12N2501/165; C12N2533/00; C12N2533/50; C12N2533/52; Y; Y10; Y10S; Y10S623/00; Y10S623/924; Y10S623/926
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
132 words
A method for implanting cells onto a prosthesis comprising the steps of: (a) providing a prosthesis comprising a porous tube, wherein at least 25% of the pores on the inner surface of said tube have diameters of more than about 40 μm and at least 25% of the pores on the outer surface of said tube have diameters of less than about 30 μm, wherein said tube comprises a substantially continuous layer of a biocompatible material, and wherein said pores on said inner surface and said pores on said outer surface are connected by gradually tapered openings; (b) contacting said prosthesis with a suspension of cells; and (c) providing a pressure differential between said inner surface and said outer surface, whereby said cells are retained in said pores of said inner surface.
Claim 2
Independent Claim
132 words
A method for implanting cells onto a prosthesis comprising the steps of: (a) providing a prosthesis comprising a porous tube, wherein at least 25% of the pores on the inner surface of said tube have diameters of more than about 40 μm and at least 25% of the pores on the outer surface of said tube have diameters of less than about 30 μm, wherein said tube comprises a substantially continuous layer of a biocompatible material, and wherein said pores on said inner surface and said pores on said outer surface are connected by gradually tapered openings; (b) contacting said prosthesis with a suspension of cells; and (c) providing a pressure differential between said inner surface and said outer surface, whereby said cells are retained in said pores of said inner surface.
Claim 3
Dependent Claim
44 words
The method of claim 1 , wherein at least 50% of said pores on said inner surface have diameters of more than about 40 μm, and at least 50% of said pores on said outer surface have diameters of less than about 30 μm.
Claim 4
Dependent Claim
44 words
The method of claim 1 , wherein at least 70% of said pores on said inner surface have diameters of more than about 40 μm, and at least 70% of said pores on said outer surface have diameters of less than about 30 μm.
Claim 5
Dependent Claim
44 words
The method of claim 1 , wherein at least 90% of said pores on said inner surface have diameters of more than about 40 μm, and at least 90% of said pores on said outer surface have diameters of less than about 30 μm.
Claim 6
Dependent Claim
44 words
The method of claim 1 , wherein at least 25% of said pores on said inner surface have diameters of more than about 50 μm, and at least 25% of said pores on said outer surface have diameters of less than about 20 μm.
Claim 7
Dependent Claim
44 words
The method of claim 1 , wherein at least 50% of said pores on said inner surface have diameters of more than about 50 μm, and at least 50% of said pores on said outer surface have diameters of less than about 20 μm.
Claim 8
Dependent Claim
44 words
The method of claim 1 , wherein at least 25% of said pores on said inner surface have diameters of more than about 60 μm, and at least 25% of said pores on said outer surface have diameters of less than about 15 μm.
Claim 9
Dependent Claim
44 words
The method of claim 1 , wherein at least 50% of said pores on said inner surface have diameters of more than about 60 μm, and at least 50% of said pores on said outer surface have diameters of less than about 15 μm.
Claim 10
Dependent Claim
13 words
The method of claim 1 , wherein said prosthesis is a vascular graft.
Claim 11
Dependent Claim
11 words
A sodded prosthesis formed by the method of claim 1 .
Claim 12
Independent Claim
158 words
A method for implanting cells onto a prosthesis comprising the steps of: (a) providing a prosthesis comprising a porous tube, wherein (i) the diameters of at least 25% of the pores on the inner surface of said tube are larger than the diameter of a human cell, such that human cells fit within said pores, (ii) the diameters of at least 25% of the pores on the outer surface of said tube are smaller than the diameter of a human cell, such that cells do not pass through said pores, (iii) said tube comprises a substantially continuous layer of a biocompatible material, and (iv) said pores on said inner surface and said pores on said outer surface are connected by gradually tapered openings; (b) contacting said prosthesis with a suspension of cells; and (c) providing a pressure differential between said inner surface and said outer surface, whereby said cells are retained in said pores of said inner surface.
Claim 13
Independent Claim
158 words
A method for implanting cells onto a prosthesis comprising the steps of: (a) providing a prosthesis comprising a porous tube, wherein (i) the diameters of at least 25% of the pores on the inner surface of said tube are larger than the diameter of a human cell, such that human cells fit within said pores, (ii) the diameters of at least 25% of the pores on the outer surface of said tube are smaller than the diameter of a human cell, such that cells do not pass through said pores, (iii) said tube comprises a substantially continuous layer of a biocompatible material, and (iv) said pores on said inner surface and said pores on said outer surface are connected by gradually tapered openings; (b) contacting said prosthesis with a suspension of cells; and (c) providing a pressure differential between said inner surface and said outer surface, whereby said cells are retained in said pores of said inner surface.
Claim 14
Dependent Claim
11 words
A sodded prosthesis formed by the method of claim 11 .
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