US6146892A
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US
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Fibrillar matrices
Assignee: University of Michigan (System)
•
Filing: 1998-09-28
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Granted: 2000-11-14
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Expires: 2018-09-28
Abstract
Abstract Methods and compositions are described that provide three-dimensional fibrillar matrices useful as, among other things, structural prosthetics and scaffolds for cells. The porous fibrillar matrices of the present invention have desirable mechanical properties suitable to a variety of applications, including platforms for in vitro cell cultivation, implants for tissue and organ engineering, implants as tendon and facia prosthetics, and product packaging.
Inventors
- 👤 Peter X. Ma
- 👤 Ruiyun Zhang
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 09:48
CPC Classifications
C; C08; C08J; C08J9/00; C08J9/28; A; A61; A61L; A61L27/00; A61L27/14; A61L27/18; A61L27/50; A61L27/56; C08J2367/00; C08J2367/04; Y; Y10; Y10S; Y10S521/00; Y10S521/913; Y10S521/916
Claims Analysis
Total Claims: 69
Claim 1
Independent Claim
73 words
A method, comprising: a) providing: i) a polymer preparation, ii) a solvent; b) mixing said polymer with said solvent to create a polymer/solvent solution; c) gelling said polymer/solvent solution; and d) treating said gelled polymer solution under conditions whereby a substantially solvent free fibrillar matrix is created having a porosity greater than approximately 80% wherein said fibrillar matrix comprises fibers having a diameter in the range of approximately 50 nm and 500 nm.
Claim 2
Independent Claim
73 words
A method, comprising: a) providing: i) a polymer preparation, ii) a solvent; b) mixing said polymer with said solvent to create a polymer/solvent solution; c) gelling said polymer/solvent solution; and d) treating said gelled polymer solution under conditions whereby a substantially solvent free fibrillar matrix is created having a porosity greater than approximately 80% wherein said fibrillar matrix comprises fibers having a diameter in the range of approximately 50 nm and 500 nm.
Claim 3
Dependent Claim
13 words
The method of claim 1, wherein said porosity is greater than approximately 85%.
Claim 4
Dependent Claim
13 words
The method of claim 1, wherein said porosity is greater than approximately 90%.
Claim 5
Dependent Claim
11 words
The method of claim 1, wherein said porosity is approximately 98%.
Claim 6
Dependent Claim
25 words
The method of claim 1, wherein said fibrillar matrix comprises fibers, wherein said fibers have a unit length between approximately 1000 nm and 2200 nm.
Claim 7
Dependent Claim
21 words
The method of claim 1, wherein said polymer preparation is selected from the group consisting of Poly(D,L-lactic acid) and Poly(L-lactic acid).
Claim 8
Dependent Claim
16 words
The method of claim 1, wherein said polymer preparation is a copolymer comprising poly(D,L-lactic acid-co-glycolic acid).
Claim 9
Dependent Claim
31 words
The method of claim 1, wherein said polymer preparation is comprised of a plurality polymers and copolymers selected from the group consisting of poly(L-lactic acid), Poly(D,L-lactic acid), and poly(D,L-lactic acid-co-glycolic acid).
Claim 10
Dependent Claim
22 words
The method of claim 1, wherein said solvent is selected from the group consisting of dioxane, tetrahydrofuran, N,N-dimethylformamide, pyridine, methanol and acetone.
Claim 11
Dependent Claim
15 words
The method of claim 1, wherein said solvent comprises a solution of dioxane and water.
Claim 12
Dependent Claim
24 words
The method of claim 1, wherein said polymer/solvent solution is contacted with compounds selected from the group consisting of salts, sugars, and water-soluble waxes.
Claim 13
Independent Claim
103 words
A method, comprising: a) providing: i) a polymer preparation, ii) a solvent b) mixing said polymer with said solvent to create a polymer solution; c) gelling said polymer/solvent solution under conditions whereby a three-dimensional fibrillar matrix is created; and d) hydrating said gelled polymer/solvent solution maintained under conditions whereby a three-dimensional fibrillar network is preserved such that said gelled polymer/solvent solution maintained under conditions whereby said preserved fibrillar three-dimensional matrix is preserved is solvent free; e) treating said hydrated solvent free gelled polymer three-dimensional fibrillar matrix under conditions whereby a solvent free fibrillar matrix is created having a porosity greater than approximately 80%.
Claim 14
Independent Claim
103 words
A method, comprising: a) providing: i) a polymer preparation, ii) a solvent b) mixing said polymer with said solvent to create a polymer solution; c) gelling said polymer/solvent solution under conditions whereby a three-dimensional fibrillar matrix is created; and d) hydrating said gelled polymer/solvent solution maintained under conditions whereby a three-dimensional fibrillar network is preserved such that said gelled polymer/solvent solution maintained under conditions whereby said preserved fibrillar three-dimensional matrix is preserved is solvent free; e) treating said hydrated solvent free gelled polymer three-dimensional fibrillar matrix under conditions whereby a solvent free fibrillar matrix is created having a porosity greater than approximately 80%.
Claim 15
Dependent Claim
13 words
The method of claim 12, wherein said porosity is greater than approximately 85%.
Claim 16
Dependent Claim
13 words
The method of claim 12, wherein said porosity is greater than approximately 90%.
Claim 17
Dependent Claim
11 words
The method of claim 12, wherein said porosity is approximately 98%.
Claim 18
Dependent Claim
21 words
The method of claim 12, wherein said fibrillar matrix comprises fibers having a diameter between approximately 50 nm and 500 nm.
Claim 19
Dependent Claim
25 words
The method of claim 12, wherein said fibrillar matrix comprises fibers, wherein said fibers have a unit length between approximately 1000 nm and 2200 nm.
Claim 20
Dependent Claim
21 words
The method of claim 12, wherein said polymer preparation is selected from the group consisting of Poly(D,L-lactic acid) and Poly(L-lactic acid).
Claim 21
Dependent Claim
16 words
The method of claim 12, wherein said polymer preparation is a copolymer comprising poly(D,L-lactic acid-co-glycolic acid).
Claim 22
Dependent Claim
31 words
The method of claim 12, wherein said polymer preparation is comprised of a plurality polymers and copolymers selected from the group consisting of poly(L-lactic acid), Poly(D,L-lactic acid), and poly(D,L-lactic acid-co-glycolic acid).
Claim 23
Dependent Claim
22 words
The method of claim 12, wherein said solvent is selected from the group consisting of dioxane, tetrahydrofuran, N,N-dimethylformamide, pyridine, methanol and acetone.
Claim 24
Dependent Claim
15 words
The method of claim 12, wherein said solvent comprises a solution of dioxane and water.
Claim 25
Dependent Claim
24 words
The method of claim 12, wherein said polymer/solvent solution is contacted with compounds selected from the group consisting of salts, sugars, and water-soluble waxes.
Claim 26
Independent Claim
118 words
A method, comprising: a) providing: i) a polymer preparation, ii) a solvent b) mixing said polymer with said solvent to create a polymer solution; c) casting said polymer/solvent solution into a desired form; d) gelling said cast polymer/solvent solution under conditions whereby a three-dimensional fibrillar matrix is created; e) hydrating said cast gelled polymer/solvent solution maintained under conditions whereby a three-dimensional fibrillar matrix is preserved such that said cast gelled polymer/solvent solution maintained under conditions whereby said preserved fibrillar three-dimensional matrix is solvent free; and f) freezing said cast hydrated solvent-free three-dimensional matrix; g) freeze-drying said cast hydrated solvent-free three-dimensional matrix such that a substantially desiccated fibrillar matrix is created having a porosity of greater than approximately 80%.
Claim 27
Independent Claim
118 words
A method, comprising: a) providing: i) a polymer preparation, ii) a solvent b) mixing said polymer with said solvent to create a polymer solution; c) casting said polymer/solvent solution into a desired form; d) gelling said cast polymer/solvent solution under conditions whereby a three-dimensional fibrillar matrix is created; e) hydrating said cast gelled polymer/solvent solution maintained under conditions whereby a three-dimensional fibrillar matrix is preserved such that said cast gelled polymer/solvent solution maintained under conditions whereby said preserved fibrillar three-dimensional matrix is solvent free; and f) freezing said cast hydrated solvent-free three-dimensional matrix; g) freeze-drying said cast hydrated solvent-free three-dimensional matrix such that a substantially desiccated fibrillar matrix is created having a porosity of greater than approximately 80%.
Claim 28
Dependent Claim
13 words
The method of claim 24, wherein said porosity is greater than approximately 85%.
Claim 29
Dependent Claim
13 words
The method of claim 24, wherein said porosity is greater than approximately 90%.
Claim 30
Dependent Claim
11 words
The method of claim 24, wherein said porosity is approximately 98%.
Claim 31
Dependent Claim
21 words
The method of claim 24, wherein said fibrillar matrix comprises fibers having a diameter between approximately 50 nm and 500 nm.
Claim 32
Dependent Claim
25 words
The method of claim 24, wherein said fibrillar matrix comprises fibers, wherein said fibers have a unit length between approximately 1000 nm and 2200 nm.
Claim 33
Dependent Claim
21 words
The method of claim 24, wherein said polymer preparation is selected from the group consisting of Poly(D,L-lactic acid) and Poly(L-lactic acid).
Claim 34
Dependent Claim
16 words
The method of claim 24, wherein said polymer preparation is a copolymer comprising poly(D,L-lactic acid-co-glycolic acid).
Claim 35
Dependent Claim
31 words
The method of claim 24, wherein said polymer preparation is comprised of a plurality polymers and copolymers selected from the group consisting of poly(L-lactic acid), Poly(D,L-lactic acid), and poly(D,L-lactic acid-co-glycolic acid).
Claim 36
Dependent Claim
22 words
The method of claim 24, wherein said solvent is selected from the group consisting of dioxane, tetrahydrofuran, N,N-dimethylformamide, pyridine, methanol and acetone.
Claim 37
Dependent Claim
15 words
The method of claim 24, wherein said solvent comprises a solution of dioxane and water.
Claim 38
Dependent Claim
24 words
The method of claim 24, wherein said polymer/solvent solution is contacted with compounds selected from the group consisting of salts, sugars, and water-soluble waxes.
Claim 39
Independent Claim
63 words
A method, comprising: a) providing: i) a polymer preparation, ii) a solvent b) mixing said polymer with said solvent to create a polymer solution; c) gelling said polymer/solvent solution at a temperature range between -195.8° C. to 23.0° C. such that a three-dimensional fibrillar matrix is created; and d) hydrating said gelled polymer/solvent solution under conditions whereby said three-dimensional fibrillar matrix is preserved.
Claim 40
Independent Claim
63 words
A method, comprising: a) providing: i) a polymer preparation, ii) a solvent b) mixing said polymer with said solvent to create a polymer solution; c) gelling said polymer/solvent solution at a temperature range between -195.8° C. to 23.0° C. such that a three-dimensional fibrillar matrix is created; and d) hydrating said gelled polymer/solvent solution under conditions whereby said three-dimensional fibrillar matrix is preserved.
Claim 41
Dependent Claim
21 words
The method of claim 36, wherein said fibrillar matrix comprises fibers having a diameter between approximately 50 nm and 500 nm.
Claim 42
Dependent Claim
25 words
The method of claim 36, wherein said fibrillar matrix comprises fibers, wherein said fibers have a unit length between approximately 1000 nm and 2200 nm.
Claim 43
Dependent Claim
16 words
The method of claim 36, wherein said temperature range is between -18° C. and 8° C.
Claim 44
Dependent Claim
21 words
The method of claim 36, wherein said polymer preparation is selected from the group consisting of Poly(D,L-lactic acid) and Poly(L-lactic acid).
Claim 45
Dependent Claim
16 words
The method of claim 36, wherein said polymer preparation is a copolymer comprising poly(D,L-lactic acid-co-glycolic acid).
Claim 46
Dependent Claim
31 words
The method of claim 36, wherein said polymer preparation is comprised of a plurality polymers and copolymers selected from the group consisting of poly(L-lactic acid), Poly(D,L,-lactic acid), and poly(D,L-lactic acid-co-glycolic acid).
Claim 47
Dependent Claim
22 words
The method of claim 36, wherein said solvent is selected from the group consisting of dioxane, tetrahydrofuran, N,N-dimethylformamide, pyridine, methanol and acetone.
Claim 48
Dependent Claim
15 words
The method of claim 36, wherein said solvent comprises a solution of dioxane and water.
Claim 49
Independent Claim
75 words
A method, comprising: a) providing: i) a polymer preparation, ii) a solvent b) mixing said polymer with said solvent to create a polymer solution; c) casting said polymer/solvent solution into a desired form; d) gelling said cast polymer/solvent solution at a temperature range between -195.8° C. to 23.0° C. such that a three-dimensional fibrillar matrix is created; and e) hydrating said cast gelled polymer/solvent solution maintained under conditions whereby a three-dimensional fibrillar matrix is preserved.
Claim 50
Independent Claim
75 words
A method, comprising: a) providing: i) a polymer preparation, ii) a solvent b) mixing said polymer with said solvent to create a polymer solution; c) casting said polymer/solvent solution into a desired form; d) gelling said cast polymer/solvent solution at a temperature range between -195.8° C. to 23.0° C. such that a three-dimensional fibrillar matrix is created; and e) hydrating said cast gelled polymer/solvent solution maintained under conditions whereby a three-dimensional fibrillar matrix is preserved.
Claim 51
Dependent Claim
21 words
The method of claim 45, wherein said fibrillar matrix comprises fibers having a diameter between approximately 50 nm and 500 nm.
Claim 52
Dependent Claim
25 words
The method of claim 45, wherein said fibrillar matrix comprises fibers, wherein said fibers have a unit length between approximately 1000 nm and 2200 nm.
Claim 53
Dependent Claim
16 words
The method of claim 45, wherein said temperature range is between -18° C. and 8° C.
Claim 54
Dependent Claim
21 words
The method of claim 45, wherein said polymer preparation is selected from the group consisting of Poly(D,L-lactic acid) and Poly(L-lactic acid).
Claim 55
Dependent Claim
16 words
The method of claim 45, wherein said polymer preparation is a copolymer comprising poly(D,L-lactic acid-co-glycolic acid).
Claim 56
Dependent Claim
31 words
The method of claim 45, wherein said polymer preparation is comprised of a plurality polymers and copolymers selected from the group consisting of poly(L-lactic acid), Poly(D,L-lactic acid), and poly(D,L-lactic acid-co-glycolic acid).
Claim 57
Dependent Claim
22 words
The method of claim 45, wherein said solvent is selected from the group consisting of dioxane, tetrahydrofuran, N,N-dimethylformamide, pyridine, methanol and acetone.
Claim 58
Dependent Claim
15 words
The method of claim 45, wherein said solvent comprises a solution of dioxane and water.
Claim 59
Independent Claim
79 words
A method, comprising: a) providing: i) a polymer preparation, ii) a solvent; b) mixing said polymer with said solvent to create a polymer/solvent solution; c) gelling said polymer/solvent solution; and d) treating said gelled polymer solution under conditions such that a substantially solvent free fibrillar matrix having a porosity greater than approximately 80% is created wherein said fibrillar matrix is comprised of fibers, said fibers having a unit length in the range of approximately 1000 nm and 2200 nm.
Claim 60
Independent Claim
79 words
A method, comprising: a) providing: i) a polymer preparation, ii) a solvent; b) mixing said polymer with said solvent to create a polymer/solvent solution; c) gelling said polymer/solvent solution; and d) treating said gelled polymer solution under conditions such that a substantially solvent free fibrillar matrix having a porosity greater than approximately 80% is created wherein said fibrillar matrix is comprised of fibers, said fibers having a unit length in the range of approximately 1000 nm and 2200 nm.
Claim 61
Dependent Claim
13 words
The method of claim 54, wherein said porosity is greater than approximately 85%.
Claim 62
Dependent Claim
13 words
The method of claim 54, wherein said porosity is greater than approximately 90%.
Claim 63
Dependent Claim
11 words
The method of claim 54, wherein said porosity is approximately 98%.
Claim 64
Dependent Claim
21 words
The method of claim 54, wherein said polymer preparation is selected from the group consisting of Poly(D,L-lactic acid) and Poly(L-lactic acid).
Claim 65
Dependent Claim
16 words
The method of claim 54, wherein said polymer preparation is a copolymer comprising poly(D,L-lactic acid-co-glycolic acid).
Claim 66
Dependent Claim
31 words
The method of claim 54, wherein said polymer preparation is comprised of a plurality polymers and copolymers selected from the group consisting of poly(L-lactic acid), Poly(D,L-lactic acid), and poly(D,L-lactic acid-co-glycolic acid).
Claim 67
Dependent Claim
22 words
The method of claim 54, wherein said solvent is selected from the group consisting of dioxane, tetrahydrofuran, N,N-dimethylformamide, pyridine, methanol and acetone.
Claim 68
Dependent Claim
15 words
The method of claim 54, wherein said solvent comprises a solution of dioxane and water.
Claim 69
Dependent Claim
24 words
The method of claim 54, wherein said polymer/solvent solution is contacted with compounds selected from the group consisting of salts, sugars, and water-soluble waxes.
References Cited (Backward Citations)
US3812224A
Cited
US5686091A
Cited
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