US5972385A
Expired / Lapsed
US
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Collagen-polysaccharide matrix for bone and cartilage repair
Assignee: DePuy Spine LLC
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Filing: 1998-01-15
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Granted: 1999-10-26
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Expires: 2018-01-15
Abstract
Abstract A matrix and a method for preparing it are provided to support the growth of tissue, such as bone, cartilage or soft tissue. A polysaccharide is reacted with an oxidizing agent to open sugar rings on the polysaccharide to form aldehyde groups. The aldehyde groups are reacted to form covalent linkages to collagen.
Inventors
- 👤 LinShu Liu
- 👤 Robert Spiro
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:07
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/14; A61L27/22; A61L27/24; A61L27/227; A61L27/26; A61L27/50; A61L27/54; A61F; A61F2310/00; A61F2310/00005; A61F2310/00365
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
60 words
A method for preparing a matrix to support the repair of tissue comprising the steps of oxidizing an exogenous polysaccharide to form a modified exogenous polysaccharide having aldehyde groups, reacting said modified exogenous polysaccharide with collagen under conditions whereby said aldehyde groups covalently react to crosslink with collagen to form said matrix and adding a growth factor to said matrix.
Claim 2
Independent Claim
60 words
A method for preparing a matrix to support the repair of tissue comprising the steps of oxidizing an exogenous polysaccharide to form a modified exogenous polysaccharide having aldehyde groups, reacting said modified exogenous polysaccharide with collagen under conditions whereby said aldehyde groups covalently react to crosslink with collagen to form said matrix and adding a growth factor to said matrix.
Claim 3
Dependent Claim
75 words
The method of claim 1 wherein said growth factor is selected from the group consisting of members of the TGF-β superfamily; members of the BMP family; the growth differentiation factors(GDF's); ADMP-1; members of the fibroblast growth factor family; members of the hedgehog family of proteins; members of the insulin-like growth factor (IGF) family; members of the platelet-derived growth factor (PDGF) family; members of the interleukin (IL) family; and members of the colony-stimulating factor (CSF) family.
Claim 4
Dependent Claim
15 words
The method of claim 2 wherein the growth factor is a bone morphogenic protein (BMP).
Claim 5
Dependent Claim
25 words
The method of claim 1 wherein the polysaccharide comprises hyaluronic acid, chondroitin sulfate, dermatan sulfate, keratan sulfate, heparan, heparan sulfate, dextran, dextran sulfate, or alginate.
Claim 6
Dependent Claim
12 words
The method according to claim 4, wherein said polysaccharide comprises hyaluronic acid.
Claim 7
Dependent Claim
22 words
The method according to claim 1 wherein the collagen is selected from the group consisting of Type 1 and Type II collagen.
Claim 8
Dependent Claim
20 words
The method according to claim 1, wherein said step of oxidizing said polysaccharide comprises treatment of said polysaccharide with periodate.
Claim 9
Dependent Claim
29 words
The method according to claim 1, wherein said collagen and said polysaccharide used to form said matrix are present in the range of 99:1 to 1:99 by weight, respectively.
Claim 10
Dependent Claim
16 words
The method according to claim 8 wherein said range is 9:1 to 1:9 by weight, respectively.
Claim 11
Dependent Claim
24 words
The method according to claim 1, wherein about 1% to 50% of the repeat units in said polysaccharide are oxidized to contain aldehyde groups.
Claim 12
Dependent Claim
24 words
The method according to claim 10, wherein about 1% to 5% of the repeat units in said polysaccharide are oxidized to contain aldehyde groups.
Claim 13
Dependent Claim
15 words
The method according to claim 1, wherein said matrix is formed by freezing and lyophilization.
Claim 14
Dependent Claim
17 words
The method according to claim 1, wherein said matrix is formed by wet laying and air drying.
Claim 15
Dependent Claim
17 words
The method of claim 1 further comprising adding fibrinogen and thrombin to form fibrin in said matrix.
Claim 16
Dependent Claim
19 words
The method of claim 1 wherein tissue is selected from the group consisting of bone, cartilage and soft tissue.
Claim 17
Dependent Claim
44 words
A matrix to support the repair of tissue, said matrix comprising a growth factor and collagen covalently crosslinked to an exogenous polysaccharide, wherein said polysaccharide is crosslinked to said collagen through oxidized sugar rings on said polysaccharide which form covalent linkages to said collagen.
Claim 18
Dependent Claim
44 words
A matrix to support the repair of tissue, said matrix comprising a growth factor and collagen covalently crosslinked to an exogenous polysaccharide, wherein said polysaccharide is crosslinked to said collagen through oxidized sugar rings on said polysaccharide which form covalent linkages to said collagen.
Claim 19
Dependent Claim
77 words
The matrix of claim 16 wherein said growth factor is selected from the group consisting of members of the TGF-β superfamily; members of the bone morphogenic protein family; the growth differentiation factors(GDF's); ADMP-1; members of the fibroblast growth factor family; members of the hedgehog family of proteins; members of the insulin-like growth factor (IGF) family; members of the platelet-derived growth factor (PDGF) family; members of the interleukin (IL) family; and members of the colony-stimulating factor (CSF) family.
Claim 20
Dependent Claim
14 words
The matrix of claim 16 wherein said growth factor is a bone morphogenic protein.
Claim 21
Dependent Claim
26 words
The matrix according to claim 16 wherein said polysaccharide comprises hyaluronic acid, chondroitin sulfate, dermatan sulfate, keratan sulfate, heparan, heparan sulfate, dextran, dextran sulfate or alginate.
Claim 22
Dependent Claim
12 words
The matrix according to claim 19 wherein said polysaccharide is hyaluronic acid.
Claim 23
Dependent Claim
26 words
The matrix according to claim 16 wherein said matrix comprises said collagen and said polysaccharide in a weight ratio in the range of 99:1 to 1:99.
Claim 24
Dependent Claim
22 words
The matrix of claim 16 wherein said collagen is selected from the group consisting of Type 1 collagen and Type 2 collagen.
Claim 25
Dependent Claim
8 words
The matrix of claim 16 further comprising fibrin.
Claim 26
Dependent Claim
31 words
A method of conducting the growth of bone or cartilage tissue in vivo comprising the step of administering a matrix according to claim 16 at a site of desired bone growth.
References Cited (Backward Citations)
US4517295A
Cited
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 2002-10-25 | 2003-04-26 | 2003-10-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-10-25 | 2007-04-26 | 2007-10-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-10-25 | 2011-04-26 | 2011-10-26 | $7700.00 | Window Closed / Lapsed |