US5945033A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for making non-crosslinked protein particles for therapeutic and diagnostic use
Assignee: PTL PHILANTHROPY LLC
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Filing: 1996-11-12
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Granted: 1999-08-31
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Expires: 2016-11-12
Abstract
Abstract Albumin particles in the nanometer and micrometer size range in an aqueous suspension are rendered stable against resolubilization without the aid of a crosslinking agent and without denaturation, by the incorporation of hemoglobin in the particle composition. Particles which are primarily hemoglobin in the nanometer and micrometer size range in an aqueous suspension are rendered stable against aggregation by the incorporation of either albumin, surface active agents or gelatin.
Inventors
- 👤 Richard C. K. Yen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:16
CPC Classifications
A; A61; A61K; A61K9/00; A61K9/48; A61K9/50; A61K9/51; A61K9/5107; A61K9/513; A61K9/5169; A61K47/00; A61K47/50; A61K47/69; A61K47/6905; A61K47/6907; A61K47/6921; A61K47/6927; A61K47/6929; A61K9/14; A61K9/16; A61K9/1605; A61K9/1611; A61K9/1617; A61K9/1629; A61K9/1658; A61K9/5192; B; B82; B82Y; B82Y5/00; Y; Y10; Y10S; Y10S977/00; Y10S977/70; Y10S977/773; Y10S977/788; Y10S977/797; Y10S977/902; Y10S977/904; Y10S977/905; Y10S977/927; Y10S977/93; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2984; Y10T428/2989
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
88 words
A method for the preparation of an aqueous suspension of monodisperse particles of non-crosslinked, non-denatured albumin, having a size range of from about 50 to about 5000 nanometers in diameter and stable against dissolving upon dilution with an alcohol-free aqueous dilution medium, said method comprising: (a) forming an aqueous solution containing albumin and hemoglobin; and (b) treating said aqueous solution with an alcohol in an amount sufficient to cause said aqueous solution to become turbid; wherein steps (a) and (b) are performed without crosslinking said albumin and hemoglobin.
Claim 2
Independent Claim
88 words
A method for the preparation of an aqueous suspension of monodisperse particles of non-crosslinked, non-denatured albumin, having a size range of from about 50 to about 5000 nanometers in diameter and stable against dissolving upon dilution with an alcohol-free aqueous dilution medium, said method comprising: (a) forming an aqueous solution containing albumin and hemoglobin; and (b) treating said aqueous solution with an alcohol in an amount sufficient to cause said aqueous solution to become turbid; wherein steps (a) and (b) are performed without crosslinking said albumin and hemoglobin.
Claim 3
Dependent Claim
33 words
A method in accordance with claim 1 in which said hemoglobin of step (a) is from about 1% to about 70% by weight relative to the total of said albumin and said hemoglobin.
Claim 4
Dependent Claim
33 words
A method in accordance with claim 1 in which said hemoglobin of step (a) is from about 1% to about 30% by weight relative to the total of said albumin and said hemoglobin.
Claim 5
Dependent Claim
16 words
A method in accordance with claim 1 in which said alcohol is a lower alkyl alcohol.
Claim 6
Dependent Claim
26 words
A method in accordance with claim 1 in which said alcohol is a member selected from the group consisting of methanol, ethanol, n-propanol, isopropanol and n-butanol.
Claim 7
Dependent Claim
23 words
A method in accordance with claim 1 in which said alcohol is a member selected from the group consisting of ethanol and n-butanol.
Claim 8
Dependent Claim
22 words
A method in accordance with claim 1 in which step (a) further comprises including in said aqueous solution an anionic water-soluble surfactant.
Claim 9
Dependent Claim
29 words
A method in accordance with claim 7 in which said anionic water-soluble surfactant is included at a concentration of at least about 0.1 g per liter of said suspension.
Claim 10
Dependent Claim
46 words
A method in accordance with claim 7 in which said anionic water-soluble surfactant is included at a concentration of from about 0.5 g to about 5 g per liter of said suspension, and said particles comprise at least about 15 g per liter of said suspension.
Claim 11
Dependent Claim
62 words
A method in accordance with claim 7 in which said anionic water-soluble surfactant is a member selected from the group consisting of sodium lauryl sulfate and sodium tetradecylsulfate, and is included at a concentration of from about 0.5 g to about 5 g per liter of said suspension, and said particles comprise at least about 15 g per liter of said suspension.
Claim 12
Dependent Claim
14 words
A method in accordance with claim 1 in which said hemoglobin is human hemoglobin.
Claim 13
Dependent Claim
15 words
A method in accordance with claim 1 in which said albumin is human serum albumin.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-08-30 | 2003-03-01 | 2003-08-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-08-30 | 2007-03-01 | 2007-08-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-08-30 | 2011-03-01 | 2011-08-31 | $7700.00 | Window Closed / Lapsed |