US5942410A
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US
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Composition and method for staining cellular DNA, comprising thiazine derivative metabisulfite and methanol or ethanol
Assignee: BC Cancer Agency
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Filing: 1997-07-07
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Granted: 1999-08-24
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Expires: 2017-07-07
Abstract
Abstract A composition and method for staining cellular DNA are disclosed. The composition of the present invention is an aqueous alcoholic solution that includes a cationic stain, a metabisulfite, and an alcohol preferably selected from methanol, ethanol, and mixtures thereof. In a preferred embodiment, the cationic stain is thionin.
Inventors
- 👤 Paul Pong-Shing Lam
- 👤 Peter William Payne
- 👤 David Michael Garner
- 👤 Branko Palcic
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-22 23:12
CPC Classifications
G; G01; G01N; G01N1/00; G01N1/28; G01N1/30; C; C09; C09B; C09B21/00; G01N15/00; G01N15/10; G01N15/14; G01N15/1429; G01N15/1433; Y; Y10; Y10S; Y10S435/00; Y10S435/975
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
53 words
A composition comprising a cationic stain having the formula: ##STR4## wherein R 1 and R 2 are independently selected from the group consisting of hydrogen and methyl, and X is a counterion; a metabisulfite; and an aqueous alcoholic solution comprising an alcohol selected from the group consisting of methanol, ethanol, and mixtures thereof.
Claim 2
Dependent Claim
53 words
A composition comprising a cationic stain having the formula: ##STR4## wherein R 1 and R 2 are independently selected from the group consisting of hydrogen and methyl, and X is a counterion; a metabisulfite; and an aqueous alcoholic solution comprising an alcohol selected from the group consisting of methanol, ethanol, and mixtures thereof.
Claim 3
Dependent Claim
13 words
The composition of claim 1 wherein R 1 and R 2 are hydrogen.
Claim 4
Dependent Claim
15 words
The composition of claim 1 wherein R 1 is methyl and R 2 is hydrogen.
Claim 5
Dependent Claim
18 words
The composition of claim 1 wherein X is selected from the group consisting of acetate, chloride, and bromide.
Claim 6
Dependent Claim
9 words
The composition of claim 1 wherein X is acetate.
Claim 7
Dependent Claim
17 words
The composition of claim 1 wherein R 1 and R 2 are hydrogen and X is acetate.
Claim 8
Dependent Claim
28 words
The composition of claim 1 wherein the cationic stain is present in the composition at a concentration of from about 1.0×10 -3 M to about 2.5×10 -2 M.
Claim 9
Dependent Claim
28 words
The composition of claim 1 wherein the cationic stain is present in the composition at a concentration of from about 4.0×10 -3 M to about 8.0×10 -3 M.
Claim 10
Dependent Claim
20 words
The composition of claim 1 wherein the metabisulfite is selected from the group consisting of lithium, sodium, and potassium metabisulfites.
Claim 11
Dependent Claim
27 words
The composition of claim 1 wherein the metabisulfite is present in the composition at a concentration of from about 1.0×10 -3 M to about 2.5×10 -2 M.
Claim 12
Dependent Claim
27 words
The composition of claim 1 wherein the metabisulfite is present in the composition at a concentration of from about 4.0×10 -3 M to about 8.0×10 -3 M.
Claim 13
Dependent Claim
18 words
The composition of claim 1 wherein the composition has a pH of from about 1.0 to about 2.0.
Claim 14
Dependent Claim
18 words
The composition of claim 1 wherein the composition has a pH of from about 1.3 to about 1.5.
Claim 15
Independent Claim
77 words
A method for staining cellular DNA comprising applying a staining solution to a cell to provide a cell having a stained nucleus, wherein the staining solution comprises a cationic stain having the formula: ##STR5## wherein R 1 and R 2 are independently selected from the group consisting of hydrogen and methyl, and X is a counterion; a metabisulfite; and an aqueous alcoholic solution comprising an alcohol selected from the group consisting of methanol, ethanol, and mixtures thereof.
Claim 16
Independent Claim
77 words
A method for staining cellular DNA comprising applying a staining solution to a cell to provide a cell having a stained nucleus, wherein the staining solution comprises a cationic stain having the formula: ##STR5## wherein R 1 and R 2 are independently selected from the group consisting of hydrogen and methyl, and X is a counterion; a metabisulfite; and an aqueous alcoholic solution comprising an alcohol selected from the group consisting of methanol, ethanol, and mixtures thereof.
Claim 17
Dependent Claim
13 words
The method of claim 14 wherein R 1 and R 2 are hydrogen.
Claim 18
Dependent Claim
15 words
The method of claim 14 wherein R 1 is methyl and R 2 is hydrogen.
Claim 19
Dependent Claim
18 words
The method of claim 14 wherein X is selected from the group consisting of acetate, chloride, and bromide.
Claim 20
Dependent Claim
17 words
The method of claim 14 wherein R 1 and R 2 are hydrogen and X is acetate.
Claim 21
Dependent Claim
28 words
The method of claim 14 wherein the cationic stain is present in the composition at a concentration of from about 1.0×10 -3 M to about 2.5×10 -2 M.
Claim 22
Dependent Claim
20 words
The method of claim 14 wherein the metabisulfite is selected from the group consisting of lithium, sodium, and potassium metabisulfites.
Claim 23
Dependent Claim
28 words
The method of claim 14 wherein the metabisulfite is present in the composition at a concentration of from about 1.033 10 -3 M to about 2.5×10 -2 M.
Claim 24
Dependent Claim
18 words
The method of claim 14 wherein the composition has a pH of from about 1.0 to about 2.0.
Claim 25
Independent Claim
97 words
A method for quantitating cellular DNA comprising: staining the cell with a composition to provide a cell having a stained nucleus, wherein the composition comprises a cationic stain having the formula: ##STR6## wherein R 1 and R 2 are selected from the group consisting of hydrogen and methyl, and X is a counterion; a metabisulfite; and an aqueous alcoholic solution comprising an alcohol selected from the group consisting of methanol, ethanol, and mixtures thereof; and measuring the optical density of the stained nucleus as an indication of the presence or amount of DNA present in the nucleus.
Claim 26
Independent Claim
97 words
A method for quantitating cellular DNA comprising: staining the cell with a composition to provide a cell having a stained nucleus, wherein the composition comprises a cationic stain having the formula: ##STR6## wherein R 1 and R 2 are selected from the group consisting of hydrogen and methyl, and X is a counterion; a metabisulfite; and an aqueous alcoholic solution comprising an alcohol selected from the group consisting of methanol, ethanol, and mixtures thereof; and measuring the optical density of the stained nucleus as an indication of the presence or amount of DNA present in the nucleus.
Claim 27
Dependent Claim
70 words
A kit for staining cellular DNA comprising a cationic stain having the formula: ##STR7## wherein R 1 and R 2 are selected from the group consisting of hydrogen and methyl, and X is a counterion; a metabisulfite; and an aqueous alcoholic solution comprising an alcohol selected from the group consisting of methanol, ethanol, and mixtures thereof; wherein the cationic stain, metabisulfite, and alcohol are contained in one or more vessels.
Claim 28
Dependent Claim
70 words
A kit for staining cellular DNA comprising a cationic stain having the formula: ##STR7## wherein R 1 and R 2 are selected from the group consisting of hydrogen and methyl, and X is a counterion; a metabisulfite; and an aqueous alcoholic solution comprising an alcohol selected from the group consisting of methanol, ethanol, and mixtures thereof; wherein the cationic stain, metabisulfite, and alcohol are contained in one or more vessels.
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| 7.5 Years (Year 8) | 2006-08-23 | 2007-02-22 | 2007-08-24 | $3760.00 | Window Closed / Lapsed |
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