US5922545A
Expired / Lapsed
US
⏱️ 0.0 years remaining
In vitro peptide and antibody display libraries
Assignee: Affymax Technologies NV
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Filing: 1997-07-29
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Granted: 1999-07-13
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Expires: 2017-07-29
Abstract
Abstract Improved methods and novel compositions for identifying peptides and single-chain antibodies that bind to predetermined receptors or epitopes. Such peptides and antibodies are identified by improved and novel methods for affinity screening of polysomes displaying nascent peptides.
Inventors
- 👤 Larry C. Mattheakis
- 👤 William J. Dower
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 06:47
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/12; C07K16/1267; C07K16/1285; C07K2317/00; C07K2317/30; C07K2317/34
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
81 words
A method for identifying multiple binding specificities of single chain antibodies, said method comprising: contacting under suitable binding conditions a multiplicity of antigen species with a polysome library displaying nascent peptides having a single chain antibody segment; and separating polysomes bound to the antigen species from polysomes not bound to the antigen species; synthesizing cDNA from the separated bound polysomes, thereby identifying single chain antibodies which bind to at least one of the antigen species present in the multiplicity of antigens.
Claim 2
Independent Claim
81 words
A method for identifying multiple binding specificities of single chain antibodies, said method comprising: contacting under suitable binding conditions a multiplicity of antigen species with a polysome library displaying nascent peptides having a single chain antibody segment; and separating polysomes bound to the antigen species from polysomes not bound to the antigen species; synthesizing cDNA from the separated bound polysomes, thereby identifying single chain antibodies which bind to at least one of the antigen species present in the multiplicity of antigens.
Claim 3
Dependent Claim
29 words
A method of claim 1, wherein the multiplicity of antigen species comprises a library of beads or pins, each bead or pin having a single species of predetermined antigen.
Claim 4
Dependent Claim
17 words
A method of claim 2, wherein the antigen species comprise polypeptides synthesized on the beads or pins.
Claim 5
Dependent Claim
33 words
A method of claim 3, wherein the beads or pins individually comprise a discrete tag capable of reporting a sequence identity of the single species of polypeptide present on the bead or pin.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-07-12 | 2003-01-11 | 2003-07-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-07-12 | 2007-01-11 | 2007-07-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-07-12 | 2011-01-11 | 2011-07-13 | $7700.00 | Window Closed / Lapsed |