US5879924A
Expired / Lapsed
US
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Immortalized cell lines for virus growth
Assignee: University of Minnesota System
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Filing: 1997-09-30
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Granted: 1999-03-09
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Expires: 2017-09-30
Abstract
Abstract This invention relates to the production and use of immortalized cell lines from primary chicken embryonic fibroblasts. The cells are useful as substrates for virus propagation, recombinant protein expression and recombinant virus production.
Inventors
- 👤 Douglas N. Foster
- 👤 Linda K. Foster
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 13:59
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/10; C12N5/06; C12N5/0602; C12N5/0652; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/86; C12N15/867; C12N7/00; C12Q; C12Q1/00; C12Q1/02; C12N2510/00; C12N2510/02; C12N2510/04; C12N2710/00; C12N2710/00011; C12N2710/16011; C12N2710/16611; C12N2710/16651; C12N2720/00; C12N2720/00011; C12N2720/12011; C12N2720/12051
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
99 words
A method for growing virus from a first cell comprising the steps of: incubating a first cell with virus; combining the first virus with immortalized chicken cells obtained by growing primary chicken embryonic fibroblasts in culture, passaging the fibroblasts in culture until they begin cell senescence, concentrating the cells during cell senescence to maintain about 30% to about 60% culture confluence, identifying foci of non-senescent cells in the culture, isolating the non-senescent cells, and growing the non-senescent cells for greater than 30 passages; and isolating virus produced from the combination of the first cell and the immortalized chicken cells.
Claim 2
Independent Claim
99 words
A method for growing virus from a first cell comprising the steps of: incubating a first cell with virus; combining the first virus with immortalized chicken cells obtained by growing primary chicken embryonic fibroblasts in culture, passaging the fibroblasts in culture until they begin cell senescence, concentrating the cells during cell senescence to maintain about 30% to about 60% culture confluence, identifying foci of non-senescent cells in the culture, isolating the non-senescent cells, and growing the non-senescent cells for greater than 30 passages; and isolating virus produced from the combination of the first cell and the immortalized chicken cells.
Claim 3
Dependent Claim
11 words
The method of claim 1 wherein the virus is a retrovirus.
Claim 4
Dependent Claim
11 words
The method of claim 1 wherein the virus is a herpesvirus.
Claim 5
Dependent Claim
12 words
The method of claim 3 wherein the virus is Marek's disease virus.
Claim 6
Dependent Claim
19 words
The method of claim 1 wherein the virus is selected from the group consisting of fowlpox virus or reovirus.
Claim 7
Dependent Claim
17 words
The method of claim 1 wherein the immortalized cells are cells identified as ATCC deposit number CRL-12203.
Claim 8
Independent Claim
107 words
A method for quantifying the amount of virus in a sample comprising the steps of: preparing at least one serial dilution of a virus; contacting a sample of virus from at least one dilution of virus with immortalized chicken cells obtained by: growing primary chicken embryonic fibroblasts in culture, passaging the fibroblasts in culture until they begin cell senescence, concentrating the cells during cell senescence to maintain about 30% to about 60% culture confluence, identifying foci of non-senescent cells in the culture, isolating the non-senescent cells, and growing the non-senescent cells for greater than 30 passages; and quantifying the amount of virus present in the virus dilution.
Claim 9
Independent Claim
107 words
A method for quantifying the amount of virus in a sample comprising the steps of: preparing at least one serial dilution of a virus; contacting a sample of virus from at least one dilution of virus with immortalized chicken cells obtained by: growing primary chicken embryonic fibroblasts in culture, passaging the fibroblasts in culture until they begin cell senescence, concentrating the cells during cell senescence to maintain about 30% to about 60% culture confluence, identifying foci of non-senescent cells in the culture, isolating the non-senescent cells, and growing the non-senescent cells for greater than 30 passages; and quantifying the amount of virus present in the virus dilution.
Claim 10
Dependent Claim
17 words
The method of claim 7 wherein the immortalized cells are cells identified as ATCC deposit number CRL-12203.
References Cited (Backward Citations)
No citation records indexed.
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