US5876957A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Methods for applying a reagent to an analytical test device
Assignee: Roche Diabetes Care Inc
•
Filing: 1998-01-08
•
Granted: 1999-03-02
•
Expires: 2018-01-08
Abstract
Abstract This invention provides methods and apparatus for applying a reagent to an analytical test device. The methods and apparatus employ a nozzle and a flexible restrictor to accurate control the amount of reagent applied to the test device. The analytical test devices prepared by the methods of this invention are used to determine the presence or the quantity of an analyte in a liquid sample, such as whole blood.
Inventors
- 👤 Joel S. Douglas
- 👤 John M. Gleisner
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 13:48
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/521; C12Q2326/00; C12Q2326/32; Y; Y10; Y10S; Y10S435/00; Y10S435/968
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
92 words
A method for applying a reagent to a test device comprising: (a) providing a test device comprising a substrate and a test pad attached to the substrate; (b) providing a fluid delivery system comprising a nozzle and a flexible restrictor attached to the nozzle for delivery of a reagent from the nozzle to the test pad; (c) applying a reagent comprising an indicator system and an oxidase enzyme or a peroxidase enzyme in a predetermined amount and at a predetermined pressure through the nozzle and the flexible restrictor to the test pad.
Claim 2
Independent Claim
92 words
A method for applying a reagent to a test device comprising: (a) providing a test device comprising a substrate and a test pad attached to the substrate; (b) providing a fluid delivery system comprising a nozzle and a flexible restrictor attached to the nozzle for delivery of a reagent from the nozzle to the test pad; (c) applying a reagent comprising an indicator system and an oxidase enzyme or a peroxidase enzyme in a predetermined amount and at a predetermined pressure through the nozzle and the flexible restrictor to the test pad.
Claim 3
Dependent Claim
16 words
The method of claim 1 wherein the fluid delivery system further comprises a pressure delivery system.
Claim 4
Dependent Claim
16 words
The method of claim 1 wherein the fluid delivery system further comprises a positive displacement system.
Claim 5
Dependent Claim
33 words
The method of claim 1 wherein a brush is attached to the underside of the nozzle and the reagent is applied through the nozzle and then through the brush to the test pad.
Claim 6
Dependent Claim
15 words
The method of claim 1 wherein the reagent is applied continuously to the test pad.
Claim 7
Dependent Claim
57 words
The method of claim 5 wherein the nozzle has an internal diameter ranging from about 0.005 to about 0.150 inches and a length ranging from about 0.2 to about 2.0 inches, and the flexible restrictor has an internal diameter ranging from about 0.002 to about 0.200 inches and a length of about 0.5 to about 12 feet.
Claim 8
Dependent Claim
18 words
The method of claim 1 wherein the reagent is applied in a discrete amount to the test pad.
Claim 9
Dependent Claim
58 words
The method of claim 7 wherein the nozzle has an internal diameter which is less than or equal to 0.032 inches and a length ranging from about 0.2 to about 2.0 inches, and the flexible restrictor has an internal diameter ranging from about 0.002 to about 0.200 inches and a length of about 0.5 to about 12 feet.
Claim 10
Dependent Claim
28 words
The method of claim 1 wherein the oxidase enzyme is selected from the group consisting of glucose oxidase, cholesterol oxidase, uricase, alcohol oxidase, aldehyde oxidase and glycerophosphate oxidase.
Claim 11
Dependent Claim
88 words
The method of claim 1 wherein the indicator system is selected from the group consisting of (a) 3-methyl-2-benzothiazolinone hydrazone hydrochloride and 3-dimethylaminobenzoic acid; (b) 3-methyl-2-benzothiazolinone hydrazone hydrochloride and 3,5-dichloro-2-hydroxybenzenesulfonic acid; (c) 4-aminoantipyrene and 5-oxo-1-(p-sulfophenyl)2-pyrazoline-3-carboxylic acid; (d) 4-aminoantipyrene and N-(m-tolyl)diethanolamine; (e) 4-aminoantipyrene and 4-methoxynaphthol; (f) 2,2'-azino-di(3-ethylbenzthiazoline)sulfonic acid; (g) pyrogallol red; (h) bromopyrogallol red; (i) acid green 25; (j) 3-methyl-2-benzothiazolinone hydrazone hydrochloride and 8-anilino-1-naphthalenesulfonate; (k) 3-methyl-2-benzothiazolinone hydrazone hydrochloride and N-(3-sulfopropyl)aniline; (l) 3-methyl-6-(sulfonate)benzothiazolinone-(2)-hydrazone sodium salt and 3-dimethylaminobenzoic acid; (m) 3-methyl-6-(sulfonate)-benzothiazolinone-(2)-hydrazone sodium salt and 8-anilino-1-naphthalenesulfonate; and (n) 3-methyl-6-(sulfonate)-benzothiazolinone(2)-hydrazone sodium salt and N-(3-sulfopropyl)aniline.
Claim 12
Dependent Claim
18 words
The method of claim 1 wherein the fluid delivery system comprises a plurality of nozzles and flexible restrictors.
Claim 13
Dependent Claim
25 words
The method of claim 11 wherein each of the plurality of nozzles and flexible restrictors applies a different concentration of reagent to the test pad.
Claim 14
Independent Claim
68 words
An apparatus for applying a reagent to a test device comprising a substrate and a test pad, said apparatus comprising: (a) a fluid delivery system comprising a nozzle and a flexible restrictor for delivery of the reagent to the test pad; (b) a support positioned under the fluid delivery system for supporting the test device; and (c) means for moving the test device under the fluid delivery system.
Claim 15
Independent Claim
68 words
An apparatus for applying a reagent to a test device comprising a substrate and a test pad, said apparatus comprising: (a) a fluid delivery system comprising a nozzle and a flexible restrictor for delivery of the reagent to the test pad; (b) a support positioned under the fluid delivery system for supporting the test device; and (c) means for moving the test device under the fluid delivery system.
Claim 16
Dependent Claim
16 words
The apparatus of claim 13 wherein the fluid delivery system further comprises a pressure delivery system.
Claim 17
Dependent Claim
16 words
The apparatus of claim 13 wherein the fluid delivery system further comprises a positive displacement system.
Claim 18
Dependent Claim
33 words
The apparatus of claim 13 wherein a brush is attached to the underside of the nozzle and the reagent is applied through the nozzle and then through the brush to the test pad.
Claim 19
Dependent Claim
57 words
The apparatus of claim 13 wherein the nozzle has an internal diameter ranging from about 0.005 to about 0.150 inches and a length ranging from about 0.2 to about 2.0 inches, and the flexible restrictor has an internal diameter ranging from about 0.002 to about 0.200 inches and a length of about 0.5 to about 12 feet.
Claim 20
Dependent Claim
58 words
The apparatus of claim 13 wherein the nozzle has an internal diameter which is less than or equal to 0.032 inches and a length ranging from about 0.2 to about 2.0 inches, and the flexible restrictor has an internal diameter ranging from about 0.002 to about 0.200 inches and a length of about 0.5 to about 12 feet.
Claim 21
Dependent Claim
16 words
The apparatus of claim 13 wherein the means for moving the test device is a conveyor.
Claim 22
Dependent Claim
18 words
The apparatus of claim 13 wherein the fluid delivery system comprises a plurality of nozzles and flexible restrictors.
References Cited (Backward Citations)
US4130085A
Cited
US4362697A
Cited
US4848606A
Cited
US4935346A
Cited
US5306623A
Cited
US5338688A
Cited
US5334247A
Cited
EP0620437A1
Cited
US5529756A
Cited
US5547702A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-03-01 | 2002-08-31 | 2003-03-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-03-01 | 2006-08-31 | 2007-03-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-03-01 | 2010-08-31 | 2011-03-02 | $7700.00 | Window Closed / Lapsed |