US5834248A
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Compositions and methods using rchd534, a gene uregulated by shear stress
Assignee: The Brigham and Women's Hospital Inc
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Filing: 1995-06-07
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Granted: 1998-11-10
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Expires: 2015-06-07
Abstract
Abstract The present invention relates to methods and compositions for the treatment and diagnosis of cardiovascular disease, including, but not limited to, atherosclerosis, ischemia/reperfusion, hypertension, restenosis, and arterial inflammation. Specifically, the present invention identifies and describes genes which are differentially expressed in cardiovascular disease states, relative to their expression in normal, or non-cardiovascular disease states, and/or in response to manipulations relevant to cardiovascular disease. Further, the present invention identifies and describes genes via the ability of their gene products to interact with gene products involved in cardiovascular disease. Still further, the present invention provides methods for the identification and therapeutic use of compounds as treatments of cardiovascular disease. Moreover, the present invention provides methods for the diagnostic monitoring of patients undergoing clinical evaluation for the treatment of cardiovascular disease, and for monitoring the efficacy of compounds in clinical trials. Additionally, the present invention describes methods for the diagnostic evaluation and prognosis of various cardiovascular diseases, and for the identification of subjects exhibiting a predisposition to such conditions.
Inventors
- 👤 Dean Falb
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 10:14
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0071; C12N9/0083; A; A61; A61P; A61P9/00; A61P9/08; A61P9/10; A61P9/12; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C12N9/0065; C12N9/0089; A61K; A61K38/00
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
50 words
An isolated polynucleotide comprising a nucleotide sequence encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO:37 or a polypeptide encoded by the rchd534 gene contained in the clone pFCHD534 deposited with the NRRL as NRRL Accession No. B-21459, or the complement of said nucleotide sequence.
Claim 2
Dependent Claim
50 words
An isolated polynucleotide comprising a nucleotide sequence encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO:37 or a polypeptide encoded by the rchd534 gene contained in the clone pFCHD534 deposited with the NRRL as NRRL Accession No. B-21459, or the complement of said nucleotide sequence.
Claim 3
Dependent Claim
44 words
An isolated polynucleotide comprising a nucleotide sequence of the rchd534 cDNA as shown in SEQ ID NO:36 or of the rchd534 cDNA insert contained in the clone pFCHD534 deposited with the NRRL as NRRL Accession No. B-21459, or the complement of said nucleotide sequence.
Claim 4
Dependent Claim
44 words
An isolated polynucleotide comprising a nucleotide sequence of the rchd534 cDNA as shown in SEQ ID NO:36 or of the rchd534 cDNA insert contained in the clone pFCHD534 deposited with the NRRL as NRRL Accession No. B-21459, or the complement of said nucleotide sequence.
Claim 5
Dependent Claim
10 words
The isolated polynucleotide of claims 1, 2 which is DNA.
Claim 6
Dependent Claim
9 words
The isolated polynucleotide of claim 3 which is cDNA.
Claim 7
Dependent Claim
10 words
The isolated polynucleotide of claim 3 which is genomic DNA.
Claim 8
Dependent Claim
12 words
The isolated polynucleotide of claims 1, 2, or 5 which is RNA.
Claim 9
Dependent Claim
15 words
The isolated polynucleotide of claims 1, 2, or 6 which further comprises a detectable label.
Claim 10
Dependent Claim
11 words
A vector containing the polynucleotide of claims 1, 2, or 7.
Claim 11
Dependent Claim
30 words
An expression vector containing the polynucleotide of claims 1, 2, or 8 in operative association with a nucleotide regulatory element that controls expression of the polynucleotide in a host cell.
Claim 12
Dependent Claim
14 words
A cultured host cell transfected with the polynucleotide of claim 1, 2 or 9.
Claim 13
Dependent Claim
29 words
A cultured host cell transfected with the polynucleotide of claim 1 in operative association with a nucleotide regulatory element that controls expression of the polynucleotide in the host cell.
Claim 14
Dependent Claim
9 words
The host cell of claim 11 which is prokaryotic.
Claim 15
Dependent Claim
9 words
The host cell of claim 11 which is eukaryotic.
Claim 16
Dependent Claim
42 words
A cultured host cell containing the polynucleotide of claim 1, 2 or 9, wherein a nucleotide regulatory element has been inserted in operative association with said polynucleotide, and wherein said nucleotide regulatory element induces overexpression of said polynucleotide in said host cell.
Claim 17
Dependent Claim
39 words
A method of producing a rchd534 polypeptide, comprising the steps of: (a) growing the host cell of claim 12 in a culture under conditions in which the rchd534 polvpeptide is expressed; and (b) collecting the polypeptide from the culture.
Claim 18
Dependent Claim
39 words
A method of producing a rchd534 polypeptide, comprising the steps of: (a) growing the host cell of claim 13 in a culture under conditions in which the rchd534 polypeptide is expressed; and (b) collecting the polypeptide from the culture.
Claim 19
Dependent Claim
61 words
An isolated polynucleotide comprising the nucleotide sequence of the rchd534 polypeptide coding region, which coding region is set forth from nucleotide residue number 1032 to 1736 of SEQ ID NO:36 or of the polypeptide coding region of the rchd534 gene contained in the clone pFCHD534 deposited with the NRRL as NRRL Accession No. B-21459, or the complement of said nucleotide sequence.
Claim 20
Dependent Claim
61 words
An isolated polynucleotide comprising the nucleotide sequence of the rchd534 polypeptide coding region, which coding region is set forth from nucleotide residue number 1032 to 1736 of SEQ ID NO:36 or of the polypeptide coding region of the rchd534 gene contained in the clone pFCHD534 deposited with the NRRL as NRRL Accession No. B-21459, or the complement of said nucleotide sequence.
References Cited (Backward Citations)
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 2001-11-09 | 2002-05-11 | 2002-11-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-11-09 | 2006-05-11 | 2006-11-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-11-09 | 2010-05-11 | 2010-11-10 | $7700.00 | Window Closed / Lapsed |