US5656441A
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Methods for determining cellular adhesion
Assignee: Boston University
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Filing: 1994-04-19
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Granted: 1997-08-12
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Expires: 2014-04-19
Abstract
Abstract This invention is directed to the measurement of distances between adherent particles and the surface to which the particles are adhered. The particles may be artificial such as beads or natural such as cells and are labeled with a detectable label. The surface may be a biological surface such as a cell, a membrane or a biological structure, or an artificial surface such as plastic or glass. The factor by which a signal emitted from particles adherent to the surface differs from the detected signal is directly related to a factor specific for each medium which can be calculated. Knowing this factor and the value of the amount of label detectable from the particles, the distance between the particle and the surface can be determined. Such methods can be used to monitor the degree of spreading of cells along a surface such as an extracellular matrix, to determine the physical nature of the cell surface, or to determine the nature of cell-to-cell and cell-to-ligand receptor bridges. These methods can also be used in testing surfaces such as tissue culture products for relative adhesiveness and in testing other products for overall stickiness or smoothness. These methods can also be used to screen chemicals, drugs, protein and many other substances to determine if they have an effect on cellular adhesion and spreading.
Inventors
- 👤 Douglas V. Faller
- 👤 Irene Ginis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:10
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/5005; G01N33/53; G01N33/569; G01N33/56966; Y; Y10; Y10T; Y10T436/00; Y10T436/13
Claims Analysis
Total Claims: 43
Claim 1
Independent Claim
123 words
A method for measuring cellular adhesion comprising the steps of: a) labeling a population of cells with a detectable label and suspending the labeled cells in a liquid medium; b) dividing the suspended cells into a plurality of wells wherein each well contains a comparable number of cells and a different volume of medium; c) detecting label from the wells of suspended cells; d) calculating a function for label detected verses volume of medium; e) calculating a quenching factor (K H ) of the medium from the function; f) adhering the suspended cells to well surfaces; and g) detecting label from the wells of adhered cells and, from the label detected and the quenching factor, measuring cellular adhesion in the population of cells.
Claim 2
Independent Claim
123 words
A method for measuring cellular adhesion comprising the steps of: a) labeling a population of cells with a detectable label and suspending the labeled cells in a liquid medium; b) dividing the suspended cells into a plurality of wells wherein each well contains a comparable number of cells and a different volume of medium; c) detecting label from the wells of suspended cells; d) calculating a function for label detected verses volume of medium; e) calculating a quenching factor (K H ) of the medium from the function; f) adhering the suspended cells to well surfaces; and g) detecting label from the wells of adhered cells and, from the label detected and the quenching factor, measuring cellular adhesion in the population of cells.
Claim 3
Dependent Claim
43 words
The method of claim 1 wherein the population of cells is selected from the group consisting of circulating cells, cells of the immune system, cells of the hematopoietic system, human cells, endothelial cells, neoplastic cells, immortalized cell lines, infected cells and combinations thereof.
Claim 4
Dependent Claim
32 words
The method of claim 1 wherein the detectable label is selected from the group consisting of florescent chemicals, luminescent chemicals, phosphorescent chemicals, chromatic chemicals, metals, radio isotopes, stable isotopes and combinations thereof.
Claim 5
Dependent Claim
17 words
The method of claim 1 wherein the label is detected after cells are subjected to electromagnetic radiation.
Claim 6
Dependent Claim
33 words
The method of claim 4 wherein the electromagnetic radiation is selected from the group consisting of gamma rays, x-rays, ultraviolet rays, visible light, infrared rays, microwaves, radio waves, electric fields and combinations thereof.
Claim 7
Dependent Claim
27 words
The method of claim 1 wherein the liquid medium is selected from the group consisting of cell culture mediums, buffered solutions, ionically balanced solutions and combinations thereof.
Claim 8
Dependent Claim
13 words
The method of claim 1 wherein the liquid medium is phosphate buffered saline.
Claim 9
Dependent Claim
13 words
The method of claim 1 wherein the wells are in a microtiter plate.
Claim 10
Dependent Claim
26 words
The method of claim 1 wherein the wells are coated with a protein selected from the group consisting of fibronectin, vitronectin, collagen, laminin and combinations thereof.
Claim 11
Dependent Claim
16 words
The method of claim 1 wherein label is detected by a detector placed below the wells.
Claim 12
Dependent Claim
16 words
The method of claim 1 wherein the suspended cells are adhered to well surfaces by centrifugation.
Claim 13
Dependent Claim
15 words
The method of claim 1 wherein cellular adhesion is measured as percent of ideal flattening.
Claim 14
Dependent Claim
30 words
The method of claim 1 further comprising the steps of binding a ligand to the surface of the labeled cells and measuring cellular adhesion in the population of ligand-bound cells.
Claim 15
Dependent Claim
29 words
The method of claim 13 wherein the ligand is selected from the group consisting of antibodies, antigens, enzymes, cytokines, pharmaceutical agents, recombinant gene products and combinations and fragments thereof.
Claim 16
Dependent Claim
25 words
The method of claim 1 further comprising the step of pre-treating the population of cells and measuring cellular adhesion in the population of pre-treated cells.
Claim 17
Dependent Claim
37 words
The method of claim 15 wherein the pre-treatment is selected from the group consisting of treatments with electromagnetic radiation, thermal treatments, chemical treatments, treatments with a pharmaceutical composition, enzymatic treatments, treatments with a cytokine and combinations thereof.
Claim 18
Dependent Claim
39 words
The method of claim 1 further comprising the step of detecting a pathological disorder in the population of cells by comparing cellular adhesion measured for the population of cells with cellular adhesion measured for a similar population of cells.
Claim 19
Dependent Claim
33 words
The method of claim 17 wherein the pathological disorder detected is selected from the group consisting of neoplasias, inflammations, immune system disorders, infections, cardiac diseases, hematopoietic system disorders and genetic defects and deficiencies.
Claim 20
Dependent Claim
27 words
The method of claim 17 wherein the similar population of cells comprises normal or diseased cells of the same tissue-type and species as the population of cells.
Claim 21
Independent Claim
190 words
A method for determining a distance between a population of cells and a surface to which the cells are adhered comprising the steps of: a) labeling the population of cells with a detectable label and suspending the labeled cells in a liquid medium; b) dividing a portion of the suspended cells into a plurality of wells wherein each well contains a different number of cells and a comparable volume of medium; c) detected label from the plurality of wells; d) lysing the cells in the plurality of wells; e) detecting label from the lysed plurality of wells; f) calculating a function of label detected from lysed and unlysed cells verses cell number; g) calculating a quenching factor (K H ) of the medium from the function; h) dividing another portion of the suspended cells into another plurality of wells; i) adhering said another portion of the suspended cells to the surface of the wells; and j) detecting label from the wells of adhered cells and, from the label detected and the quenching factor, determining the distance between the population of cells and the surface to which the cells are adhered.
Claim 22
Independent Claim
190 words
A method for determining a distance between a population of cells and a surface to which the cells are adhered comprising the steps of: a) labeling the population of cells with a detectable label and suspending the labeled cells in a liquid medium; b) dividing a portion of the suspended cells into a plurality of wells wherein each well contains a different number of cells and a comparable volume of medium; c) detected label from the plurality of wells; d) lysing the cells in the plurality of wells; e) detecting label from the lysed plurality of wells; f) calculating a function of label detected from lysed and unlysed cells verses cell number; g) calculating a quenching factor (K H ) of the medium from the function; h) dividing another portion of the suspended cells into another plurality of wells; i) adhering said another portion of the suspended cells to the surface of the wells; and j) detecting label from the wells of adhered cells and, from the label detected and the quenching factor, determining the distance between the population of cells and the surface to which the cells are adhered.
Claim 23
Dependent Claim
16 words
The method of claim 20 wherein the population of cells is obtained from a diseased patient.
Claim 24
Dependent Claim
16 words
The method of claim 20 wherein the cells are adhered to well surfaces before being lysed.
Claim 25
Dependent Claim
16 words
The method of claim 20 wherein the cells are lysed by the addition of a detergent.
Claim 26
Dependent Claim
21 words
The method of claim 23 wherein the detergent is selected from the group consisting of anionic, cationic, zwitterionic and non-ionic detergents.
Claim 27
Independent Claim
65 words
A method for determining surface adhesion comprising the steps of: a) labeling a collection of particles with a detectable label and suspending the labeled particles in a fluid medium with a known or determinable quenching factor; b) adhering the labeled particles to the surface; and c) measuring label detected from the adhered particles and, from the label detected and the quenching factor, determining surface adhesion.
Claim 28
Independent Claim
65 words
A method for determining surface adhesion comprising the steps of: a) labeling a collection of particles with a detectable label and suspending the labeled particles in a fluid medium with a known or determinable quenching factor; b) adhering the labeled particles to the surface; and c) measuring label detected from the adhered particles and, from the label detected and the quenching factor, determining surface adhesion.
Claim 29
Dependent Claim
24 words
The method of claim 25 wherein the surface is selected from the group consisting of ceramics, glasses, plastics, metals, biological surfaces and combinations thereof.
Claim 30
Dependent Claim
13 words
The method of claim 25 wherein the surface is a tissue culture product.
Claim 31
Dependent Claim
23 words
The method of claim 25 wherein the particles are selected from the group consisting of beads, crystals, liposomes, artificial cells and biological cells.
Claim 32
Dependent Claim
35 words
The method of claim 25 wherein labeling is performed by a process selected from the group consisting of organic chemical reactions, enzymatic labeling, biosynthetic labeling, hydrophobic interaction, ionic interaction, conjugation, covalent modification and osmotic incorporation.
Claim 33
Dependent Claim
17 words
The method of claim 25 further comprising the step of treating the surface before adhering said particles.
Claim 34
Dependent Claim
22 words
The method of claim 25 wherein the treatment is selected from the group consisting of chemical treatments, enzymatic treatments and combinations thereof.
Claim 35
Dependent Claim
26 words
The method of claim 25 wherein the treatment is selected from the group consisting of physical treatments, treatments with electromagnetic radiation, heat treatments and combinations thereof.
Claim 36
Independent Claim
103 words
A method for measuring adhesion of particles comprising the steps of: a) labeling the particles with a detectable label and suspending the labeled particles in a fluid; b) dividing the suspended particles into a plurality of samples wherein each sample contains a different number of particles in a comparable volume of fluid; c) detecting label from the samples; d) calculating a function for label detected verses particle number; e) calculating a quenching factor (K H ); f) adhering the suspended particles to a surface; and g) detecting label from the adhered particles and, from the label detected and the quenching factor, measuring adhesion.
Claim 37
Independent Claim
103 words
A method for measuring adhesion of particles comprising the steps of: a) labeling the particles with a detectable label and suspending the labeled particles in a fluid; b) dividing the suspended particles into a plurality of samples wherein each sample contains a different number of particles in a comparable volume of fluid; c) detecting label from the samples; d) calculating a function for label detected verses particle number; e) calculating a quenching factor (K H ); f) adhering the suspended particles to a surface; and g) detecting label from the adhered particles and, from the label detected and the quenching factor, measuring adhesion.
Claim 38
Dependent Claim
23 words
The method of claim 33 wherein the particles are selected from the group consisting of beads, crystals, liposomes, artificial cells and biological cells.
Claim 39
Dependent Claim
10 words
The method of claim 33 wherein the particles are coated.
Claim 40
Dependent Claim
30 words
The method of claim 35 wherein the coating is selected from the group consisting of antibodies, cytokines, enzymes, recombinant proteins, matrices, adhesion molecules, lipids, polysaccharides, synthetic polymers and combinations thereof.
Claim 41
Dependent Claim
32 words
The method of claim 33 wherein the detectable label is selected from the group consisting of florescent chemicals, luminescent chemicals, phosphorescent chemicals, chromatic chemicals, metals, radio isotopes, stable isotopes and combinations thereof.
Claim 42
Dependent Claim
24 words
The method of claim 33 wherein the surface is selected from the group consisting of ceramics, glasses, plastics, metals, biological surfaces and combinations thereof.
Claim 43
Dependent Claim
37 words
The method of claim 33 wherein the particles are labeled by a process selected from the group consisting of organic chemical reactions, encapsulation, ionic interactions, hydrophobic interactions, covalent modifications, conjugations, osmotic incorporation, physical manipulations and combinations thereof.
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| 7.5 Years (Year 8) | 2004-08-11 | 2005-02-10 | 2005-08-12 | $3760.00 | Window Closed / Lapsed |
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