Patent US5725777A successfully refreshed from Google Patents.
US5620850A
Expired / Lapsed
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Molecular recognition at surfaces derivatized with self-assembled monolayers
Assignee: Harvard University
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Filing: 1994-09-26
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Granted: 1997-04-15
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Expires: 2014-09-26
Abstract
Abstract An article suitable for use as a biosensor includes a species of a formula X-R-Ch adhered to a surface of the article as part of a self-assembled monolayer. X is a functionality that adheres to the surface, R is a spacer moiety, and Ch is a chelating agent. A metal ion can be coordinated by the chelating agent, and a polyamino acid-tagged biological binding partner of a target biological molecule coordinated to the metal ion. A method of the invention involves bringing the article into contact with a medium containing or suspected of containing the target biological molecule and allowing the biological molecule to biologically bind to the binding partner. The article is useful particularly as a surface plasmon resonance chip.
Inventors
- 👤 Cynthia C. Bamdad
- 👤 George B. Sigal
- 👤 Jack L. Strominger
- 👤 George M. Whitesides
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 13:01
CPC Classifications
B; B82; B82Y; B82Y30/00; C; C07; C07H; C07H21/00; C07H21/04; B82Y15/00; B82Y40/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6834; C12Q1/6837; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54353; G01N33/54366; G01N33/54373; G01N33/551; G01N33/553; G01N2610/00
Claims Analysis
Total Claims: 31
Claim 1
Independent Claim
106 words
A species having a formula: X--R--Ch--M wherein X represents a functional group and R represents a spacer moiety that, together, are able to promote formation from solution of a self-assembled monolayer on a surface, Ch represents a chelating agent that coordinates a metal ion, and M represents a metal ion coordinated to the chelating agent, wherein M also is coordinated to a biological binding partner of a biological molecule via coordination sites not filled by the chelating agent or includes coordination sites not filled by the chelating agent that, upon exposure to a polyamino acid tag, are able to become coordinated by the polyamino acid tag.
Claim 2
Dependent Claim
106 words
A species having a formula: X--R--Ch--M wherein X represents a functional group and R represents a spacer moiety that, together, are able to promote formation from solution of a self-assembled monolayer on a surface, Ch represents a chelating agent that coordinates a metal ion, and M represents a metal ion coordinated to the chelating agent, wherein M also is coordinated to a biological binding partner of a biological molecule via coordination sites not filled by the chelating agent or includes coordination sites not filled by the chelating agent that, upon exposure to a polyamino acid tag, are able to become coordinated by the polyamino acid tag.
Claim 3
Dependent Claim
16 words
The species as recited in claim 1, wherein the chelating agent is a quadradentate chelating agent.
Claim 4
Dependent Claim
14 words
The species as recited in claim 1, wherein the chelating agent is nitrilotriacetic acid.
Claim 5
Dependent Claim
32 words
The species as recited in claim 1, wherein the chelating agent and metal ion are selected such that the chelating agent coordinates all but at least two of the metal coordination sites.
Claim 6
Dependent Claim
26 words
The species as recited in claim 1, wherein the chelating agent is a tetradentate chelating agent, and the metal ion has a coordination number of six.
Claim 7
Dependent Claim
15 words
The species as recited in claim 1, wherein the metal ion is Ni 2+ .
Claim 8
Dependent Claim
22 words
The species as recited in claim 1, further comprising a biological binding partner of a biological molecule coordinated to the metal ion.
Claim 9
Dependent Claim
22 words
The species as recited in claim 7, wherein the biological binding partner includes a polyamino acid tag that coordinates the metal ion.
Claim 10
Dependent Claim
28 words
The species as recited in claim 8, wherein the polyamino acid tag comprises at least two neighboring amino acids defining a chelating agent that coordinates the metal ion.
Claim 11
Dependent Claim
21 words
The species as recited in claim 9, wherein the at least two neighboring amino acids are at least two neighboring histidines.
Claim 12
Dependent Claim
16 words
The species as recited in claim 7, wherein the biological binding partner specifically binds a protein.
Claim 13
Dependent Claim
19 words
A species as recited in claim 1, wherein X represents a functional group that adheres to a gold surface.
Claim 14
Dependent Claim
15 words
A species as recited in claim 12, wherein X--R--Ch represents a thiol-linked nitrilotriacetic acid chelate.
Claim 15
Dependent Claim
119 words
A species having a formula: X--R--Ch--M wherein X represents a functional group and R represents a spacer moiety that, together, are able to promote formation from solution of a self-assembled monolayer on a gold surface, Ch represents a chelating agent that coordinates a metal ion, selected from the group consisting of bidentate, tridentate, and quadradentate chelating agents, and M represents a metal ion coordinated to the chelating agent, wherein M also is coordinated to a biological binding partner of a biological molecule via coordination sites not filled by the chelating agent or includes coordination sites not filled by the chelating agent that, upon exposure to a polyamino acid tag, are able to become coordinated by the polyamino acid tag.
Claim 16
Dependent Claim
119 words
A species having a formula: X--R--Ch--M wherein X represents a functional group and R represents a spacer moiety that, together, are able to promote formation from solution of a self-assembled monolayer on a gold surface, Ch represents a chelating agent that coordinates a metal ion, selected from the group consisting of bidentate, tridentate, and quadradentate chelating agents, and M represents a metal ion coordinated to the chelating agent, wherein M also is coordinated to a biological binding partner of a biological molecule via coordination sites not filled by the chelating agent or includes coordination sites not filled by the chelating agent that, upon exposure to a polyamino acid tag, are able to become coordinated by the polyamino acid tag.
Claim 17
Dependent Claim
26 words
The species as recited in claim 14, wherein the chelating agent is a quadradentate chelating agent, and the metal ion has a coordination number of six.
Claim 18
Dependent Claim
15 words
The species as recited in claim 14, wherein the metal ion is Ni 2+ .
Claim 19
Dependent Claim
14 words
The species as recited in claim 14, wherein the chelating agent is nitrilotriacetic acid.
Claim 20
Dependent Claim
15 words
A species as recited in claim 14, wherein X--R--Ch represents a thiol-linked nitrilotriacetic acid chelate.
Claim 21
Dependent Claim
22 words
The species as recited in claim 14, further comprising a biological binding partner of a biological molecule coordinated to the metal ion.
Claim 22
Dependent Claim
16 words
The species as recited in claim 19, wherein the biological binding partner specifically binds a protein.
Claim 23
Dependent Claim
70 words
A species having a formula: X--R--Ch--M--BP wherein X represents a functional group and R represents a spacer moiety that, together, are able to promote formation from solution of a self-assembled monolayer on a surface, Ch represents a chelating agent that coordinates a metal ion, M represents a metal ion coordinated to the chelating agent, and BP represents a biological binding partner of a biological molecule coordinated to the metal ion.
Claim 24
Dependent Claim
70 words
A species having a formula: X--R--Ch--M--BP wherein X represents a functional group and R represents a spacer moiety that, together, are able to promote formation from solution of a self-assembled monolayer on a surface, Ch represents a chelating agent that coordinates a metal ion, M represents a metal ion coordinated to the chelating agent, and BP represents a biological binding partner of a biological molecule coordinated to the metal ion.
Claim 25
Dependent Claim
22 words
The species as recited in claim 21, wherein the biological binding partner includes a polyamino acid tag that coordinates the metal ion.
Claim 26
Dependent Claim
28 words
The species as recited in claim 22, wherein the polyamino acid tag comprises at least two neighboring amino acids defining a chelating agent that coordinates the metal ion.
Claim 27
Dependent Claim
21 words
The species as recited in claim 23, wherein the at least two neighboring amino acids are at least two neighboring histidines.
Claim 28
Dependent Claim
16 words
The species as recited in claim 21, wherein the biological binding partner specifically binds a protein.
Claim 29
Dependent Claim
33 words
The species as recited in claim 21, wherein the biological binding partner specifically binds a protein and includes a polyamino acid tag comprising at least two neighboring histidines that coordinates the metal ion.
Claim 30
Dependent Claim
19 words
The species as recited in claim 21, wherein X represents a functional group that adheres to a gold surface.
Claim 31
Dependent Claim
15 words
A species as recited in claim 21, wherein X--R--Ch represents a thiol-linked nitrilotriacetic acid chelate.
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| 3.5 Years (Year 4) | 2000-04-14 | 2000-10-14 | 2001-04-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-04-14 | 2004-10-14 | 2005-04-15 | $3760.00 | Window Closed / Lapsed |
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