Patent US2105273A successfully refreshed from Google Patents.
US5610041A
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Processes for producing polyhydroxybutyrate and related polyhydroxyalkanoates in the plastids of higher plants
Assignee: Michigan State University
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Filing: 1994-06-06
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Granted: 1997-03-11
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Expires: 2014-06-06
Abstract
Abstract The present invention relates to a process for producing poly-D-(-)-3-hydroxybutyric acid (PHB) and related polyhydroxyalkanoates (PHA) in the plastids of plants. The production of PHB is accomplished by genetically transforming plants with modified genes from microorganisms. The genes encode the enzymes required to synthesize PHB from acetyl-CoA or related metabolites and are fused with additional plant sequences for targeting the enzymes to the plastid.
Inventors
- 👤 Christopher R. Somerville
- 👤 Christiane Nawrath
- 👤 Yves Poirier
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:47
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0006; C12N15/00; C12N15/09; C12N15/11; C12N15/52; C12N15/63; C12N15/79; C12N15/82; C12N15/8241; C12N15/8242; C12N15/8243; C12N9/10; C12N9/1025; C12N9/1029; C12P; C12P7/00; C12P7/62; C12P7/625
Claims Analysis
Total Claims: 49
Claim 1
Independent Claim
82 words
A transgenic plant material having plastids, the plant material containing foreign DNA encoding a bacterial polypeptide which is selected from the group consisting of 3 ketothiolase, acetoacetyl-CoA reductase and polyhydroxyalkanoate (PHA) synthase and mixtures thereof leading to the production of a polyhydroxyalkanoate in the plastid in the plant material; wherein the bacterial polypeptide encoded by the foreign DNA is operably fused at its amino terminus to a transit peptide which effects targeting of the polypeptide into the plastid of the plant material.
Claim 2
Dependent Claim
82 words
A transgenic plant material having plastids, the plant material containing foreign DNA encoding a bacterial polypeptide which is selected from the group consisting of 3 ketothiolase, acetoacetyl-CoA reductase and polyhydroxyalkanoate (PHA) synthase and mixtures thereof leading to the production of a polyhydroxyalkanoate in the plastid in the plant material; wherein the bacterial polypeptide encoded by the foreign DNA is operably fused at its amino terminus to a transit peptide which effects targeting of the polypeptide into the plastid of the plant material.
Claim 3
Dependent Claim
12 words
The plant of claim 1 wherein the polyhydroxyalkanoate (PHA) is polyhydroxybutyrate (PHB).
Claim 4
Dependent Claim
39 words
The plant material of claim 2 wherein coding sequence of the DNA and RNA for the production of the enzymes leading to polyhydroxybutyrate (PHB) synthesis are as shown in SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3.
Claim 5
Dependent Claim
59 words
A transgenic plant material having plastids, the plant material containing foreign DNA encoding a bacterial polypeptide which exhibits 3-ketothiolase activity in the plastid in the plant material; wherein bacterial polypeptide encoded by the foreign DNA is operably fused at its amino terminus to a transit peptide which effects targeting of the polypeptide into the plastid of the plant material.
Claim 6
Dependent Claim
59 words
A transgenic plant material having plastids, the plant material containing foreign DNA encoding a bacterial polypeptide which exhibits 3-ketothiolase activity in the plastid in the plant material; wherein bacterial polypeptide encoded by the foreign DNA is operably fused at its amino terminus to a transit peptide which effects targeting of the polypeptide into the plastid of the plant material.
Claim 7
Dependent Claim
21 words
The plant material of claim 4 wherein the DNA which contains an open reading frame is shown in SEQ ID NO:1.
Claim 8
Dependent Claim
61 words
A transgenic plant material having plastids, the plant material containing foreign DNA encoding a bacterial polypeptide which exhibits acetoacetyl-CoA reductase activity in the plastid of the plant material; wherein the bacterial polypeptide encoded by the foreign DNA is operably fused at its amino terminus to a transit peptide which effects targeting of the polypeptide into the plastid of the plant material.
Claim 9
Dependent Claim
61 words
A transgenic plant material having plastids, the plant material containing foreign DNA encoding a bacterial polypeptide which exhibits acetoacetyl-CoA reductase activity in the plastid of the plant material; wherein the bacterial polypeptide encoded by the foreign DNA is operably fused at its amino terminus to a transit peptide which effects targeting of the polypeptide into the plastid of the plant material.
Claim 10
Dependent Claim
22 words
The transgenic plant material of claim 6 wherein the DNA which contains an open reading frame is shown in SEQ ID NO:2.
Claim 11
Dependent Claim
61 words
A transgenic plant material having plastids, the plant material containing foreign DNA encoding a bacterial polypeptide which exhibits PHA synthase activity in the plastid of the plant material; wherein the bacterial polypeptide encoded by the foreign DNA is operably fused at its amino terminus to a transit peptide which effects targeting of the polypeptide into the plastid of the plant material.
Claim 12
Dependent Claim
61 words
A transgenic plant material having plastids, the plant material containing foreign DNA encoding a bacterial polypeptide which exhibits PHA synthase activity in the plastid of the plant material; wherein the bacterial polypeptide encoded by the foreign DNA is operably fused at its amino terminus to a transit peptide which effects targeting of the polypeptide into the plastid of the plant material.
Claim 13
Dependent Claim
68 words
A transgenic plant material having plastids, the plant material containing foreign DNA encoding one or more bacterial enzymes leading to the synthesis of polyhydroxyalkanoate (PHA) from hydroxyacyl-CoA in the plastid of the plant material; wherein each of said enzymes encoded by the foreign DNA is operably fused at its amino terminus to a transit peptide which effects targeting of the enzyme into the plastid of the plant material.
Claim 14
Dependent Claim
68 words
A transgenic plant material having plastids, the plant material containing foreign DNA encoding one or more bacterial enzymes leading to the synthesis of polyhydroxyalkanoate (PHA) from hydroxyacyl-CoA in the plastid of the plant material; wherein each of said enzymes encoded by the foreign DNA is operably fused at its amino terminus to a transit peptide which effects targeting of the enzyme into the plastid of the plant material.
Claim 15
Dependent Claim
14 words
The plant material of claim 9 wherein the DNA is in SEQ ID NO:3.
Claim 16
Dependent Claim
64 words
A transgenic plant material having plastids, the plant material containing foreign DNA encoding one or more bacterial enzymes which catalyze synthesis of hydroxyacyl-CoA in the plastid of the plant material; wherein each of the said enzymes encoded by foreign DNA is operably fused at its amino terminus to a transit peptide which effects targeting of the enzyme into the plastid of the plant material.
Claim 17
Dependent Claim
64 words
A transgenic plant material having plastids, the plant material containing foreign DNA encoding one or more bacterial enzymes which catalyze synthesis of hydroxyacyl-CoA in the plastid of the plant material; wherein each of the said enzymes encoded by foreign DNA is operably fused at its amino terminus to a transit peptide which effects targeting of the enzyme into the plastid of the plant material.
Claim 18
Dependent Claim
68 words
A transgenic plant having plastids, the plant containing foreign DNA encoding one or more bacterial enzymes leading to production of acetoacetyl-CoA, from products encoded by the foreign DNA, in the plastid of the plant; wherein each of said enzymes encoded by the foreign DNA is operably fused at its amino terminus to a transit peptide which effects targeting of the enzyme into the plastid of the plant material.
Claim 19
Dependent Claim
68 words
A transgenic plant having plastids, the plant containing foreign DNA encoding one or more bacterial enzymes leading to production of acetoacetyl-CoA, from products encoded by the foreign DNA, in the plastid of the plant; wherein each of said enzymes encoded by the foreign DNA is operably fused at its amino terminus to a transit peptide which effects targeting of the enzyme into the plastid of the plant material.
Claim 20
Dependent Claim
15 words
The plant material of claim 1 as an embryo, seed or propagule of the seed.
Claim 21
Dependent Claim
15 words
The plant material of claim 2 as an embryo, seed or propagule of the seed.
Claim 22
Dependent Claim
15 words
The plant material of claim 3 as an embryo, seed or propagule of the seed.
Claim 23
Dependent Claim
15 words
The plant material of claim 4 as am embryo, seed or propagule of the seed.
Claim 24
Dependent Claim
15 words
The plant material of claim 5 as an embryo, seed or propagule of the seed.
Claim 25
Dependent Claim
15 words
The plant material of claim 6 as an embryo, seed or propagule of the seed.
Claim 26
Dependent Claim
15 words
The plant material of claim 7 as an embryo, seed or propagule of the seed.
Claim 27
Dependent Claim
15 words
The plant material of claim 8 as an embryo, seed or propagule of the seed.
Claim 28
Dependent Claim
15 words
The plant material of claim 10 as an embryo, seed or propagule of the seed.
Claim 29
Dependent Claim
15 words
The plant material of claim 11 as an embryo, seed or propagule of the seed.
Claim 30
Dependent Claim
15 words
The plant cell of claim 12 as an embryo, seed or propagule of the seed.
Claim 31
Independent Claim
106 words
A method for introducing foreign DNA encoding polypeptides leading to the synthesis of a polyhydroxyalkanoate (PHA) in a plastid in a plant which comprises mating by sexual fertilization two plants which do not produce PHA, each containing foreign DNA from a bacterium encoding one or more different enzymes in a pathway leading to polymerization of hydroxyacyl-CoA by PHA synthase to produce the plant which synthesizes the PHA in a plastid in the plant; wherein each of said enzymes encoded by the foreign DNA is operably fused at its amino terminus to a transit peptide which effects targeting of the enzyme into the plastid of the plant.
Claim 32
Independent Claim
106 words
A method for introducing foreign DNA encoding polypeptides leading to the synthesis of a polyhydroxyalkanoate (PHA) in a plastid in a plant which comprises mating by sexual fertilization two plants which do not produce PHA, each containing foreign DNA from a bacterium encoding one or more different enzymes in a pathway leading to polymerization of hydroxyacyl-CoA by PHA synthase to produce the plant which synthesizes the PHA in a plastid in the plant; wherein each of said enzymes encoded by the foreign DNA is operably fused at its amino terminus to a transit peptide which effects targeting of the enzyme into the plastid of the plant.
Claim 33
Dependent Claim
11 words
The method of claim 24 wherein the PHA is polyhydroxybutyrate (PHB).
Claim 34
Dependent Claim
53 words
A gene segment as contained in plasmid pBI-TPSS-Thio or pBIB-CCN-Thio, the gene segment encoding a modified 3-ketothiolase polypeptide for targeting to a plastid of a plant; wherein the 3-ketothiolase polypeptide is operably fused at its amino terminus to a transit peptide which effects targeting of the polypeptide into the plastid of the plant.
Claim 35
Dependent Claim
53 words
A gene segment as contained in plasmid pBI-TPSS-Thio or pBIB-CCN-Thio, the gene segment encoding a modified 3-ketothiolase polypeptide for targeting to a plastid of a plant; wherein the 3-ketothiolase polypeptide is operably fused at its amino terminus to a transit peptide which effects targeting of the polypeptide into the plastid of the plant.
Claim 36
Dependent Claim
9 words
A plant containing the gene segment of claim 26.
Claim 37
Dependent Claim
9 words
The plant of claim 27 which is Arabidopsis thaliana.
Claim 38
Dependent Claim
56 words
A gene segment as contained in plasmid pBI-TPSS-Red from Alcaligenes eutrophus, the gene segment encoding a modified acetoacetyl-CoA reductase polypeptide for targeting to a plastid of a plant; wherein the acetoacetyl-CoA reductase polypeptide is operably fused at its amino terminus to a transit peptide which effects targeting of the polypeptide into the plastid of the plant.
Claim 39
Dependent Claim
56 words
A gene segment as contained in plasmid pBI-TPSS-Red from Alcaligenes eutrophus, the gene segment encoding a modified acetoacetyl-CoA reductase polypeptide for targeting to a plastid of a plant; wherein the acetoacetyl-CoA reductase polypeptide is operably fused at its amino terminus to a transit peptide which effects targeting of the polypeptide into the plastid of the plant.
Claim 40
Dependent Claim
9 words
A plant containing the gene segment of claim 29.
Claim 41
Dependent Claim
9 words
The plant of claim 30 which is Arabidopsis thaliana.
Claim 42
Dependent Claim
59 words
A gene segment as contained in plasmid pBI-TPSS-Syn or pBIB-HCN-Syn from Alcaligenes eutrophus, the gene segment encoding a modified polyhydroxybutyrate (PHB) synthase polypeptide for targeting to a plastid of a plant; wherein the polyhydroxybutyrate synthase polypeptide is operably fused at its amino terminus to a transit peptide which effects targeting of the polypeptide into the plastid of the plant.
Claim 43
Dependent Claim
59 words
A gene segment as contained in plasmid pBI-TPSS-Syn or pBIB-HCN-Syn from Alcaligenes eutrophus, the gene segment encoding a modified polyhydroxybutyrate (PHB) synthase polypeptide for targeting to a plastid of a plant; wherein the polyhydroxybutyrate synthase polypeptide is operably fused at its amino terminus to a transit peptide which effects targeting of the polypeptide into the plastid of the plant.
Claim 44
Dependent Claim
9 words
A plant containing the gene segment of claim 32.
Claim 45
Dependent Claim
9 words
The plant of claim 24 which is Arabidopsis thaliana.
Claim 46
Dependent Claim
71 words
A transgenic plant material having plastids, the plant material containing foreign DNA encoding one or more bacterial enzymes leading to production of 3-hydroxybutyryl-CoA, from products encoded by the foreign DNA, in the plastid in the plant material; wherein the each of said enzymes incoded by foreign DNA is operably fused at its amino terminus to a transit peptide which effects targeting of the polypeptide into the plastid of the plant material.
Claim 47
Dependent Claim
71 words
A transgenic plant material having plastids, the plant material containing foreign DNA encoding one or more bacterial enzymes leading to production of 3-hydroxybutyryl-CoA, from products encoded by the foreign DNA, in the plastid in the plant material; wherein the each of said enzymes incoded by foreign DNA is operably fused at its amino terminus to a transit peptide which effects targeting of the polypeptide into the plastid of the plant material.
Claim 48
Independent Claim
93 words
A method for producing polyhydroxyalkanoate comprising: a) mating by sexual fertilization two plants which do not produce PHA, each containing foreign DNA from a bacterium encoding one or more different enzymes in a pathway leading to polymerization of hydroxyacyl-CoA by PHA synthase to produce the plant which synthesizes the PHA in a plastid in the plant; wherein the each of said enzymes encoded by foreign DNA is operably fused at its amino terminus to a transit peptide which effects targeting of the polypeptide into the plastid of the plant. b) recovering the polyhydroxyalkanoate.
Claim 49
Independent Claim
93 words
A method for producing polyhydroxyalkanoate comprising: a) mating by sexual fertilization two plants which do not produce PHA, each containing foreign DNA from a bacterium encoding one or more different enzymes in a pathway leading to polymerization of hydroxyacyl-CoA by PHA synthase to produce the plant which synthesizes the PHA in a plastid in the plant; wherein the each of said enzymes encoded by foreign DNA is operably fused at its amino terminus to a transit peptide which effects targeting of the polypeptide into the plastid of the plant. b) recovering the polyhydroxyalkanoate.
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 2000-03-10 | 2000-09-09 | 2001-03-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-03-10 | 2004-09-09 | 2005-03-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-03-10 | 2008-09-09 | 2009-03-11 | $7700.00 | Window Closed / Lapsed |