US5466596A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Tissue specific transcriptional regulatory element
Assignee: Mount Sinai Hospital
•
Filing: 1992-08-25
•
Granted: 1995-11-14
•
Expires: 2012-08-25
Abstract
Abstract A novel transcriptional regulatory element which is capable of directing expression of a gene specifically in cells of the endothelial lineage. The transcriptional regulatory element may be used to target expression of a gene in cells of the endothelial lineage.
Inventors
- 👤 Martin L. Breitman
- 👤 Daniel Dumont
- 👤 Gerard G. Gradwohl
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:46
CPC Classifications
A; A01; A01K; A01K67/00; A01K67/027; A01K67/0275; A61; A61P; A61P35/00; A61P43/00; A61P9/00; A61P9/08; A61P9/10; A61P9/12; C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N9/00; C12N9/10; C12N9/12; C12N9/1205; A01K2217/00; A01K2217/05; A61K; A61K48/00
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
32 words
An isolated transcriptional regulatory element comprising (a) a nucleotide sequence having nucleotides 1 to 560 of the sequence as shown in SEQ. ID. NO. 5, and (b) nucleotide sequences complementary to (a).
Claim 2
Dependent Claim
32 words
An isolated transcriptional regulatory element comprising (a) a nucleotide sequence having nucleotides 1 to 560 of the sequence as shown in SEQ. ID. NO. 5, and (b) nucleotide sequences complementary to (a).
Claim 3
Dependent Claim
20 words
A recombinant DNA molecule comprising a transcriptional regulatory element as claimed in claim 1 and a gene operatively linked thereto.
Claim 4
Dependent Claim
16 words
A recombinant DNA molecule as claimed in claim 2 wherein the gene is a reporter gene.
Claim 5
Dependent Claim
22 words
A recombinant DNA molecule as claimed in claim 2 wherein the gene encodes a toxic or therapeutic substance or an angiogenic factor.
Claim 6
Dependent Claim
23 words
A recombinant DNA molecule comprising a transcriptional regulatory element as claimed in claim 1 operatively linked to a gene and a reporter gene.
Claim 7
Dependent Claim
68 words
A recombinant DNA molecule as claimed in claim 5 wherein the reporter gene is a lacZ gene which codes for β-galactosidase, a neo gene which codes for neomycin phosphotransferase, a cat gene which codes for chloramphenicol acetyltransferase, a dhfr gene which codes for dihydrofolate reductase, a aphIV gene which codes for hygromycin phosphotransferase, a lux gene which codes for luciferase, or a uidA gene which codes for β-glucuronidase.
Claim 8
Dependent Claim
33 words
A recombinant DNA molecule comprising a transcriptional regulatory element as claimed in claim 1 operatively linked to a gene encoding a toxic or therapeutic substance or an angiogenic factor, and a reporter gene.
Claim 9
Dependent Claim
24 words
A cell line comprising transformant animal host cells including a recombinant DNA molecule as claimed in claim 2, 3, 4, 5, 7 or 6.
Claim 10
Dependent Claim
25 words
A cell line comprising transformant mouse endothelial host cells including a recombinant DNA molecule as claimed in claim 2, 3, 4, 5, 7 or 6.
Claim 11
Dependent Claim
25 words
A cell line comprising transformant human endothelial host cells including a recombinant DNA molecule as claimed in claim 2, 3, 4, 5, 6 or 7.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-11-13 | 1999-05-15 | 1999-11-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-11-13 | 2003-05-15 | 2003-11-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-11-13 | 2007-05-15 | 2007-11-14 | $7700.00 | Window Closed / Lapsed |