US5457047A
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DNA Sequences coding for PTH variants, PTH variants, expression vector, bacterial host, use and therapeutic composition
Assignee: Helmholtz Centre for Infection Research
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Filing: 1990-02-23
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Granted: 1995-10-10
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Expires: 2010-02-23
Abstract
Abstract PCT No. PCT/EP90/00312 Sec. 371 Date Aug. 16, 1990 Sec. 102(e) Date Aug. 16, 1990 PCT Filed Feb. 23, 1990 PCT Pub. No. WO90/10067 PCT Pub. Date Sep. 7, 1990.The invention relates to DNA sequences coding for PTH variants, to PTH variants, expression vectors, bacterial hosts, uses and therapeutic compositions.
Inventors
- 👤 Edgar Wingender
- 👤 Heiko Mielke
- 👤 Gisela Bercz-Timm
- 👤 Klaus-Dieter Schluter
- 👤 Hubert Mayer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 07:48
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/635; A; A61; A61P; A61P3/00; A61P43/00; A61K; A61K38/00; C07K2319/00; C07K2319/61; C07K2319/70; C07K2319/74; C07K2319/75
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
67 words
DNA sequence that codes for a variant of PTH(1-84), in which one or two amino acids are exchanged for another amino acid in the region of positions 28 to 31, and which is optionally lengthened N-terminally (position +1) by one or two amino acids the amino acids coded by the DNA sequence not being arranged in an order that occurs in bPTH, pPTH, rPTH, cPTH or hPTH.
Claim 2
Dependent Claim
67 words
DNA sequence that codes for a variant of PTH(1-84), in which one or two amino acids are exchanged for another amino acid in the region of positions 28 to 31, and which is optionally lengthened N-terminally (position +1) by one or two amino acids the amino acids coded by the DNA sequence not being arranged in an order that occurs in bPTH, pPTH, rPTH, cPTH or hPTH.
Claim 3
Dependent Claim
102 words
DNA sequence that codes for a variant of PTH(1-84), in which one or two amino acids are exchanged for another amino acid in the region of positions 32 to 34, except that tyrosine may not appear at position 34, and, further which is optionally lengthened N-terminally (position +1) by one or two amino acids or is shortened C-terminally (position +84) by one amino acid or is optionally shortened N-terminally (position +1) and C-terminally (position +84) by one amino acid, the amino acids coded by the DNA sequence not being arranged in a sequence that occurs in bPTH, pPTH, rPTH, cPTH or hPTH.
Claim 4
Dependent Claim
102 words
DNA sequence that codes for a variant of PTH(1-84), in which one or two amino acids are exchanged for another amino acid in the region of positions 32 to 34, except that tyrosine may not appear at position 34, and, further which is optionally lengthened N-terminally (position +1) by one or two amino acids or is shortened C-terminally (position +84) by one amino acid or is optionally shortened N-terminally (position +1) and C-terminally (position +84) by one amino acid, the amino acids coded by the DNA sequence not being arranged in a sequence that occurs in bPTH, pPTH, rPTH, cPTH or hPTH.
Claim 5
Dependent Claim
101 words
DNA sequence that codes for a variant of PTH(1-84), in which one or two amino acids are exchanged for another amino acid in the region of positions 30 to 34, except that tyrosine may not appear at position 34, and which is optionally lengthened N-terminally (position +1) by one or two amino acids or is shortened C-terminally (position +84) by one amino acid, or is optionally shortened N-terminally (position +1) and C-terminally (position +84) by one amino acid, the amino acids coded by the DNA sequence not being arranged in a sequence that occurs in bPTH, pPTH, rPTH, cPTH or hPTH.
Claim 6
Dependent Claim
101 words
DNA sequence that codes for a variant of PTH(1-84), in which one or two amino acids are exchanged for another amino acid in the region of positions 30 to 34, except that tyrosine may not appear at position 34, and which is optionally lengthened N-terminally (position +1) by one or two amino acids or is shortened C-terminally (position +84) by one amino acid, or is optionally shortened N-terminally (position +1) and C-terminally (position +84) by one amino acid, the amino acids coded by the DNA sequence not being arranged in a sequence that occurs in bPTH, pPTH, rPTH, cPTH or hPTH.
Claim 7
Dependent Claim
114 words
DNA sequence that codes for a variant of PTH(1-4), in which (a) the region of positions 28 to 34 is unchanged or (b) one or two amino acids are exchanged for another amino acid in the region of positions 28 to 34 except that tyrosine may not appear at position 34 and, in addition to (a) or (b), one or two amino acids are exchanged for another amino acid outside the region of positions 28 to 34, and which is optionally lengthened N-terminally (position +1) by one or two amino acids the amino acids coded by the DNA sequence not being arranged in a sequence that occurs in bPTH, pPTH, rPTH, cPTH or hPTH.
Claim 8
Dependent Claim
114 words
DNA sequence that codes for a variant of PTH(1-4), in which (a) the region of positions 28 to 34 is unchanged or (b) one or two amino acids are exchanged for another amino acid in the region of positions 28 to 34 except that tyrosine may not appear at position 34 and, in addition to (a) or (b), one or two amino acids are exchanged for another amino acid outside the region of positions 28 to 34, and which is optionally lengthened N-terminally (position +1) by one or two amino acids the amino acids coded by the DNA sequence not being arranged in a sequence that occurs in bPTH, pPTH, rPTH, cPTH or hPTH.
Claim 9
Dependent Claim
62 words
DNA sequence that codes for a variant of PTH(1-84), in which one or two amino acids are exchanged for another amino acid in the region of positions 32 to 34, except that tyrosine may not appear at position 34, the amino acids coded by the DNA sequence not being arranged in a sequence that occurs in bPTH, pPTH, rPTH, cPTH or hPTH.
Claim 10
Dependent Claim
62 words
DNA sequence that codes for a variant of PTH(1-84), in which one or two amino acids are exchanged for another amino acid in the region of positions 32 to 34, except that tyrosine may not appear at position 34, the amino acids coded by the DNA sequence not being arranged in a sequence that occurs in bPTH, pPTH, rPTH, cPTH or hPTH.
Claim 11
Dependent Claim
62 words
DNA sequence that codes for a variant of PTH(1-84), in which one or two amino acids are exchanged for another amino acid in the region of positions 30 to 34, except that tyrosine may not appear at position 34, the amino acids coded by the DNA sequence not being arranged in a sequence that occurs in bPTH, pPTH, rPTH, cPTH or hPTH.
Claim 12
Dependent Claim
62 words
DNA sequence that codes for a variant of PTH(1-84), in which one or two amino acids are exchanged for another amino acid in the region of positions 30 to 34, except that tyrosine may not appear at position 34, the amino acids coded by the DNA sequence not being arranged in a sequence that occurs in bPTH, pPTH, rPTH, cPTH or hPTH.
Claim 13
Dependent Claim
100 words
DNA sequence that codes for a variant of PTH(1-84), in which (a) the region of positions 28 to 34 is unchanged or (b) one or two amino acids are exchanged for another amino acid in the region of positions 28 to 34 except that tyrosine may not appear at position 34 and, in addition to (a) or (b), one or two amino acids are exchanged for another amino acid outside the region of positions 28 to 34 the amino acids coded by the DNA sequence not being arranged in a sequence that occurs in bPTH, pPTH, rPTH, cPTH or hPTH.
Claim 14
Dependent Claim
100 words
DNA sequence that codes for a variant of PTH(1-84), in which (a) the region of positions 28 to 34 is unchanged or (b) one or two amino acids are exchanged for another amino acid in the region of positions 28 to 34 except that tyrosine may not appear at position 34 and, in addition to (a) or (b), one or two amino acids are exchanged for another amino acid outside the region of positions 28 to 34 the amino acids coded by the DNA sequence not being arranged in a sequence that occurs in bPTH, pPTH, rPTH, cPTH or hPTH.
Claim 15
Dependent Claim
25 words
DNA sequence according to any one of claims 1, 2, 3, or 4 which codes for a PTH variant that is lengthened N-terminally by proline.
Claim 16
Dependent Claim
19 words
A DNA sequence according to claims 4 or 7, wherein said region is of positions of 30 to 34.
Claim 17
Dependent Claim
32 words
Bacterial expression vectors having a DNA sequence according to any one of claims 1, 2, 3, 4, 5, 6, 7 or 9 for the expression of a peptide in a bacterial host.
Claim 18
Dependent Claim
31 words
A DNA sequence according to any one of claims 1, 2, 3, 4, 5, 6, 7 or 9, which codes for a variant protein of hPTH, bPTH, pPTH, rPTH or cPTH.
Claim 19
Dependent Claim
10 words
Bacterial host having an expression vector according to claim 10.
Claim 20
Dependent Claim
14 words
Bacterial expression vectors according to claim 10 wherein Escherichia coli is the bacterial host.
References Cited (Backward Citations)
US4423037A
Cited
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