US5429938A
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Methods and compositions for isolation and growth of kidney tubule stem cells, in vitro kidney tubulogenesis and ex vivo construction of renal tubules
Assignee: University of Michigan-Ann Arbor
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Filing: 1992-03-02
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Granted: 1995-07-04
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Expires: 2012-03-02
Abstract
Abstract Methods, including culture media conditions, which provide for isolation and purification of renal tubule stem cells and for in vitro kidney tubulogenesis are disclosed. The methods rely on culturing adult kidney cells in a culture media treated with combinations of transforming growth factor-β 1 , epidermal growth factor, and all-trans retinoic acid.
Inventors
- 👤 H. David Humes
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:37
CPC Classifications
A; A61; A61K; A61K38/00; C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0684; C12N5/0687; A61P; A61P13/00; A61P13/02; A61P15/00; C12N5/0686; A61K35/00; A61K35/12; A61K48/00; C12N2501/00; C12N2501/10; C12N2501/11; C12N2501/15; C12N2501/30; C12N2501/38; C12N2501/385; C12N2502/00; C12N2502/28; C12N2510/00; C12N2533/00; C12N2533/50; C12N2533/54; Y; Y10; Y10S; Y10S623/00; Y10S623/915
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
89 words
An ex vivo renal tubule tissue system prepared by a process comprising culturing adult kidney cells in a culture medium comprising all-trans retinoic acid, transforming growth factor-β 1 and either epidermal growth factor or transforming growth factor-α in an amount effective for achieving tubulogenesis, wherein tubulogenesis is a phenotypic transformation of said cells such that condensed aggregates of tubule cells form about a central lumen wherein said lumen is bordered by cells possessing a polarized epithelial phenotype with extensive microvilli formation and tight junctional complexes along the lumenal border.
Claim 2
Dependent Claim
89 words
An ex vivo renal tubule tissue system prepared by a process comprising culturing adult kidney cells in a culture medium comprising all-trans retinoic acid, transforming growth factor-β 1 and either epidermal growth factor or transforming growth factor-α in an amount effective for achieving tubulogenesis, wherein tubulogenesis is a phenotypic transformation of said cells such that condensed aggregates of tubule cells form about a central lumen wherein said lumen is bordered by cells possessing a polarized epithelial phenotype with extensive microvilli formation and tight junctional complexes along the lumenal border.
Claim 3
Dependent Claim
34 words
The renal tubule tissue system of claim 1, wherein said culture medium contains an insoluble factor selected from the group consisting of Type I collagen, Type IV collagen, laminin, proteoglycans, fibronectin, and combinations thereof.
Claim 4
Dependent Claim
32 words
The renal tubule tissue system of claim 1, wherein said culture medium comprises a soluble factor selected from the group consisting of fetal calf serum, prostaglandins, hydrocortisone, triodothyronine, selenium and combinations thereof.
Claim 5
Dependent Claim
54 words
The renal tubule tissue system of claim 1, wherein said culture medium comprises a soluble factor selected from the group consisting of fetal calf serum, prostaglandins, hydrocortisone, triodothyronine, selenium and combinations thereof, and an insoluble factor selected from the group consisting of Type I collagen, Type IV collagen, laminin, proteoglycans, fibronectin, and combinations thereof.
Claim 6
Independent Claim
88 words
A method for growing kidney tubule stem cells ex vivo, comprising culturing adult kidney cells in the presence of all-trans retinoic acid, transforming growth factor-β 1 , and either epidermal growth factor or transforming growth factor-α, in an amount effective for achieving tubulogenesis, wherein tubulogenesis is a phenotypic transformation of said cells such that condensed aggregates of tubule cells form about a central lumen wherein said lumen is bordered by cells possessing a polarized epithelial phenotype with extensive microvilli formation and tight junctional complexes along the lumenal border.
Claim 7
Independent Claim
88 words
A method for growing kidney tubule stem cells ex vivo, comprising culturing adult kidney cells in the presence of all-trans retinoic acid, transforming growth factor-β 1 , and either epidermal growth factor or transforming growth factor-α, in an amount effective for achieving tubulogenesis, wherein tubulogenesis is a phenotypic transformation of said cells such that condensed aggregates of tubule cells form about a central lumen wherein said lumen is bordered by cells possessing a polarized epithelial phenotype with extensive microvilli formation and tight junctional complexes along the lumenal border.
Claim 8
Dependent Claim
31 words
The method of claim 5, wherein said culture medium contains an insoluble factor selected from the group consisting of Type I collagen, Type IV collagen, laminin, proteoglycans, fibronectin, and combinations thereof.
Claim 9
Dependent Claim
29 words
The method of claim 5, wherein said culture medium comprises a soluble factor selected from the group consisting of fetal calf serum, prostaglandins, hydrocortisone, triodothyronine, selenium and combinations thereof.
Claim 10
Dependent Claim
51 words
The method of claim 5, wherein said culture medium contains a soluble factor selected from the group consisting of fetal calf serum, prostaglandins, hydrocortisone, triodothyronine, selenium and combinations thereof, and an insoluble factor selected from the group consisting of Type I collagen, Type IV collagen, laminin, proteoglycans, fibronectin, and combinations thereof.
Claim 11
Independent Claim
90 words
A method for effecting tubulogenesis in a renal cell culture ex vivo, comprising culturing adult kidney cells in the presence of all-trans retinoic acid, transforming growth factor-β 1 , and either epidermal growth factor or transforming growth factor-α in an amount effective for achieving tubulogenesis, wherein tubulogenesis is a phenotypic transformation of said cells such that condensed aggregates of tubule cells form about a central lumen wherein said lumen is bordered by cells possessing a polarized epithelial phenotype with extensive microvilli formation and tight junctional complexes along the lumenal border.
Claim 12
Independent Claim
90 words
A method for effecting tubulogenesis in a renal cell culture ex vivo, comprising culturing adult kidney cells in the presence of all-trans retinoic acid, transforming growth factor-β 1 , and either epidermal growth factor or transforming growth factor-α in an amount effective for achieving tubulogenesis, wherein tubulogenesis is a phenotypic transformation of said cells such that condensed aggregates of tubule cells form about a central lumen wherein said lumen is bordered by cells possessing a polarized epithelial phenotype with extensive microvilli formation and tight junctional complexes along the lumenal border.
Claim 13
Dependent Claim
31 words
The method of claim 9, wherein said culture medium contains an insoluble factor selected from the group consisting of Type I collagen, Type IV collagen, laminin, proteoglycans, fibronectin, and combinations thereof.
Claim 14
Dependent Claim
29 words
The method of claim 9, wherein said culture medium contains a soluble factor selected from the group consisting of fetal calf serum, prostaglandins, hydrocortisone, triodothyronine, selenium and combinations thereof.
Claim 15
Dependent Claim
51 words
The method of claim 9, wherein said culture medium contains a soluble factor selected from the group consisting of fetal calf serum, prostaglandins, hydrocortisone, triodothyronine, selenium and combinations thereof, and an insoluble factor selected from the group consisting of Type I collagen, Type IV collagen, laminin, proteoglycans, fibronectin, and combinations thereof.
Claim 16
Independent Claim
91 words
A method for constructing and maintaining an ex vivo renal tubule tissue system, comprising culturing adult kidney cells in the presence of all-trans retinoic acid, transforming growth factor-β 1 , and either epidermal growth factor or transforming growth factor-α in an amount effective for achieving tubulogenesis, wherein tubulogenesis is aa phenotypic transformation of said cells characterized by condensed aggregates of tubule cells forming about a central lumen wherein said lumen is bordered by cells possessing a polarized epithelial phenotype with extensive microvilli formation and tight junctional complexes along the lumenal border.
Claim 17
Independent Claim
91 words
A method for constructing and maintaining an ex vivo renal tubule tissue system, comprising culturing adult kidney cells in the presence of all-trans retinoic acid, transforming growth factor-β 1 , and either epidermal growth factor or transforming growth factor-α in an amount effective for achieving tubulogenesis, wherein tubulogenesis is aa phenotypic transformation of said cells characterized by condensed aggregates of tubule cells forming about a central lumen wherein said lumen is bordered by cells possessing a polarized epithelial phenotype with extensive microvilli formation and tight junctional complexes along the lumenal border.
Claim 18
Dependent Claim
31 words
The method of claim 13, wherein said culture medium contains an insoluble factor selected from the group consisting of Type I collagen, Type IV collagen, laminin, proteoglycans, fibronectin, and combinations thereof.
Claim 19
Dependent Claim
29 words
The method of claim 13, wherein said culture medium contains a soluble factor selected from the group consisting of fetal calf serum, prostaglandins, hydrocortisone, triodothyronine, selenium and combinations thereof.
Claim 20
Dependent Claim
51 words
The method of claim 13, wherein said culture medium contains a soluble factor selected from the group consisting of fetal calf serum, prostaglandins, hydrocortisone, triodothyronine, selenium and combinations thereof, and an insoluble factor selected from the group consisting of Type I collagen, Type IV collagen, laminin, proteoglycans, fibronectin, and combinations thereof.
References Cited (Backward Citations)
No citation records indexed.
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