US5418162A
Expired / Lapsed
US
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Serotonin transporter CDNA
Assignee: DUEK UNIVERSITY
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Filing: 1992-10-14
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Granted: 1995-05-23
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Expires: 2012-10-14
Abstract
Abstract Isolated DNA encoding a serotonin transporter is disclosed. Also disclosed are vectors and host cells containing the aforesaid DNA, methods of using the same, purified protein by the same, and oligonucleotides and antibodies which bind thereto. Specific embodiments are cDNAs encoding rat and human serotonin transporter.
Inventors
- 👤 Randy D. Blakely
- 👤 Marc G. Caron
- 👤 Robert T. Fremeau, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 10:13
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
89 words
Isolated DNA selected from the group consisting of: (a) isolated DNA comprising SEQ ID NO: 6; (b) isolated DNA which hybridizes to the complementary strand of isolated DNA of (a) above under stringent hybridization conditions represented by a wash stringency of 0.3 Molar NaCl, 0.03M sodium citrate, 0.1% SDS at 60° C., and which encodes a serotonin transporter; and (c) isolated DNA differing from the isolated DNAs of (a) and (b) above in codon sequence due to the degeneracy of the genetic code, and which encodes a serotonin transporter.
Claim 2
Dependent Claim
89 words
Isolated DNA selected from the group consisting of: (a) isolated DNA comprising SEQ ID NO: 6; (b) isolated DNA which hybridizes to the complementary strand of isolated DNA of (a) above under stringent hybridization conditions represented by a wash stringency of 0.3 Molar NaCl, 0.03M sodium citrate, 0.1% SDS at 60° C., and which encodes a serotonin transporter; and (c) isolated DNA differing from the isolated DNAs of (a) and (b) above in codon sequence due to the degeneracy of the genetic code, and which encodes a serotonin transporter.
Claim 3
Dependent Claim
11 words
Isolated DNA according to claim 1 which encodes rat serotonin transporter.
Claim 4
Dependent Claim
17 words
Isolated DNA according to claim 1, which encodes the rat serotonin transporter given in SEQ ID NO:9.
Claim 5
Dependent Claim
9 words
A DNA vector comprising DNA according to claim 1.
Claim 6
Dependent Claim
14 words
A DNA vector according to claim 4, wherein said vector DNA comprises a plasmid.
Claim 7
Dependent Claim
14 words
A DNA vector according to claim 4, wherein said vector DNA comprises a virus.
Claim 8
Dependent Claim
14 words
A DNA vector according to claim 4, wherein said vector DNA comprises a baculovirus.
Claim 9
Dependent Claim
59 words
A transformed host cell containing a recombinant DNA sequence comprising vector DNA and DNA selected from the group consisting of: (a) isolated DNA consisting essentially of SEQ ID NO: 6; and (b) isolated DNA differing from the isolated DNA of (a) above in codon sequence due to the degeneracy of the genetic code, and which encodes a serotonin transporter.
Claim 10
Dependent Claim
59 words
A transformed host cell containing a recombinant DNA sequence comprising vector DNA and DNA selected from the group consisting of: (a) isolated DNA consisting essentially of SEQ ID NO: 6; and (b) isolated DNA differing from the isolated DNA of (a) above in codon sequence due to the degeneracy of the genetic code, and which encodes a serotonin transporter.
Claim 11
Dependent Claim
68 words
A host cell containing a recombinant DNA sequence comprising vector DNA and DNA selected from the group consisting of: (a) isolated DNA consisting essentially of SEQ ID NO: 6; and (b) isolated DNA differing from the isolated DNA of (a) above in codon sequence due to the degeneracy of the genetic code, and which encodes a serotonin transporter, said host cell capable of expressing the encoded serotonin transporter.
Claim 12
Dependent Claim
68 words
A host cell containing a recombinant DNA sequence comprising vector DNA and DNA selected from the group consisting of: (a) isolated DNA consisting essentially of SEQ ID NO: 6; and (b) isolated DNA differing from the isolated DNA of (a) above in codon sequence due to the degeneracy of the genetic code, and which encodes a serotonin transporter, said host cell capable of expressing the encoded serotonin transporter.
Claim 13
Dependent Claim
15 words
A host cell according to claim 9, wherein said host cell is a mammalian cell.
Claim 14
Dependent Claim
15 words
A host cell according to claim 9, wherein said host cell is an insect cell.
Claim 15
Dependent Claim
38 words
An oligonucleotide probe capable of selectively hybridizing, under stringent hybridization conditions represented by a wash stringency of 0.3 Molar NaCl, 0.03M sodium citrate, 0.1% SDS at 60° C., to a DNA consisting a portion of SEQ ID NO.10.
Claim 16
Dependent Claim
38 words
An oligonucleotide probe capable of selectively hybridizing, under stringent hybridization conditions represented by a wash stringency of 0.3 Molar NaCl, 0.03M sodium citrate, 0.1% SDS at 60° C., to a DNA consisting a portion of SEQ ID NO.10.
Claim 17
Dependent Claim
18 words
An oligonucleotide probe according to claim 12, which probe is capable of serving as a PCR extension primer.
Claim 18
Dependent Claim
15 words
An oligonucleotide probe according to claim 12, which probe is labelled with a detectable group.
Claim 19
Dependent Claim
14 words
An oligonucleotide probe according to claim 14, which detectable group is a radioactive atom.
References Cited (Backward Citations)
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| 11.5 Years (Year 12) | 2006-05-22 | 2006-11-21 | 2007-05-23 | $7700.00 | Window Closed / Lapsed |