US5378616A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Mutant Escherichia coli capable of enhanced L-glutamic acid production by fermentation
Assignee: Ajinomoto Co Inc
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Filing: 1992-08-07
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Granted: 1995-01-03
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Expires: 2012-08-07
Abstract
Abstract The present invention provides a mutant and a process for producing L-glutamic acid by fermentation using a microorganism belonging to the genus Escherichia. In the present process, L-glutamic acid is produced and accumulated in a culture medium by (A) culturing an Escherichia mutant which is deficient or low in α-ketoglutaric acid dehydrogenase activity, has low L-glutamic acid decomposing ability, and is capable of producing L-glutamic acid.
Inventors
- 👤 Nobuharu Tujimoto
- 👤 Yoshimi Kikuchi
- 👤 Osamu Kurahashi
- 👤 Yoshiko Kawahara
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:06
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/14; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/848; Y10S435/849
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
75 words
A process for producing L-glutamic acid by fermentation which comprises: (A) culturing in a culture medium, a mutant which is derived from Escherichia coli K-12 strain, wherein said mutant (i) is deficient or low in α-KGDH activity, (ii) is low in glutamic acid decomposition activity, (iii) is able to produce L-glutamic acid and (iv) expresses a malate synthase, an isocitrate lyase and an isocitrate dehydrogenase kinase/phosphatase; and (B) isolating L-glutamic acid from the culture medium.
Claim 2
Dependent Claim
75 words
A process for producing L-glutamic acid by fermentation which comprises: (A) culturing in a culture medium, a mutant which is derived from Escherichia coli K-12 strain, wherein said mutant (i) is deficient or low in α-KGDH activity, (ii) is low in glutamic acid decomposition activity, (iii) is able to produce L-glutamic acid and (iv) expresses a malate synthase, an isocitrate lyase and an isocitrate dehydrogenase kinase/phosphatase; and (B) isolating L-glutamic acid from the culture medium.
Claim 3
Dependent Claim
11 words
A process of claim 1, wherein said mutant is FERM P-12379.
References Cited (Backward Citations)
EP0030391A2
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-01-02 | 1998-07-04 | 1999-01-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-01-02 | 2002-07-04 | 2003-01-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-01-02 | 2006-07-04 | 2007-01-03 | $7700.00 | Window Closed / Lapsed |