US5371002A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of production of poly-beta-hydroxyalkanoate copolymers
Assignee: James Madison University
•
Filing: 1991-09-27
•
Granted: 1994-12-06
•
Expires: 2011-09-27
Abstract
Abstract The invention related to recombinant deoxyribonucleic acid (DNA) technology and, more particularly, to a process whereby poly-beta-hydroxyalkanoate (PHA) copolymers can be synthesized in a recombinant host strain containing the poly-beta-hydroxybutyrate (PHB) biosynthetic genes of Alcaligenes eutrophus.
Inventors
- 👤 Douglas E. Dennis
- 👤 Steven C. Slater
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:25
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/93; C07; C07K; C07K14/00; C07K14/195; C07K14/225; C12N15/00; C12N15/09; C12N15/11; C12N15/52; C12P; C12P7/00; C12P7/62; C12P7/625
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
70 words
A method for producing poly-beta-hydroxyalkanoate copolymers comprising: (a) introducing into an Escherichia coli host cell which expresses enzymes of acetate utilization constituitively, a vector containing a DNA sequence coding for a poly-beta-hydroxybutyrate biosynthetic pathway; (b) culturing the host cell on a substrate comprising a carbon source and propionate; (c) obtaining expression of the poly-betahydroxybutyrate biosynthetic pathway in the host cell; and, (d) recovering poly-beta-hydroxyalkanoate copolymers produced by the host cell.
Claim 2
Independent Claim
70 words
A method for producing poly-beta-hydroxyalkanoate copolymers comprising: (a) introducing into an Escherichia coli host cell which expresses enzymes of acetate utilization constituitively, a vector containing a DNA sequence coding for a poly-beta-hydroxybutyrate biosynthetic pathway; (b) culturing the host cell on a substrate comprising a carbon source and propionate; (c) obtaining expression of the poly-betahydroxybutyrate biosynthetic pathway in the host cell; and, (d) recovering poly-beta-hydroxyalkanoate copolymers produced by the host cell.
Claim 3
Dependent Claim
20 words
The method of claim 1 wherein said DNA sequence coding for the poly-beta-hydroxybutyrate biosynthetic pathway is obtained from Alcaligenes eutrophus.
Claim 4
Dependent Claim
12 words
The method of claim 1 in which the vector is a plasmid.
Claim 5
Dependent Claim
28 words
The method of claim 3 wherein said plasmid is p4A which is deposited with the American Type Culture Collection E. coli HMS 174 and given Accession No. 68329.
Claim 6
Dependent Claim
19 words
The method of claim 1 in which the carbon source comprises glucose, fructose, sucrose, lactose, maltose or mixtures thereof.
Claim 7
Dependent Claim
25 words
The method of claim 1 in which the propionate in the substrate is present in a concentration from about 2.5 mM to about 100 mM.
Claim 8
Dependent Claim
14 words
The method of claim 1 in which the poly-betahydroxyalkanoate copolymers comprise poly-beta-hydroxybutyrate and poly-betahydroxyvalerate.
Claim 9
Dependent Claim
22 words
The method of claim 1 in which the poly-betahydroxyalkanoate copolymers comprise approximately 10-50% polybeta-hydroxyvalerate, based on total percentage of poly-betahydroxyalkanoate copolymers produced.
References Cited (Backward Citations)
EP0052459A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-12-05 | 1998-06-06 | 1998-12-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-12-05 | 2002-06-06 | 2002-12-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-12-05 | 2006-06-06 | 2006-12-06 | $7700.00 | Window Closed / Lapsed |