US5312758A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and device for combined enrichment, processing and embedding of cytological specimens according to histological principles
Assignee: SED-PAR-SED A FINNISH CORP Oy AB
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Filing: 1990-09-05
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Granted: 1994-05-17
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Expires: 2010-09-05
Abstract
Abstract A method and device for the processing of cytological specimens. The device comprises a preferably cone-shaped receptacle with a closed bottom part, sidewalls defining an upper aperture with a surface area larger than that of the bottom part, and a perforated membrane adapted to cover the aperture of the receptacle for retaining the cytological specimens. The membrane is preferably a mesh having a mesh size of up to 80 microns, the hydraulic conductance of the membrane being sufficient to allow the exchange of processing fluids when the receptacle is inserted into a tissue processing apparatus. The sidewalls form an obtuse angle with the bottom in order to promote such an exchange. In the method, the receptacle with specimens is inserted into a tissue processor with the membrane in a vertical position. After processing and paraffination, the receptacle is cooled allowing the specimens to sedimentate to form an enriched layer at the bottom of the receptacle. The solid paraffin block with enriched layer is thereafter removed from the receptacle and cut on a microtome.
Inventors
- 👤 Johan E. W. Ahlqvist
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:59
CPC Classifications
G; G01; G01N; G01N1/00; G01N1/28; G01N1/36; G01N1/30; G01N1/31; G01N2001/315; Y; Y10; Y10T; Y10T436/00; Y10T436/25; Y10T436/2525
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
202 words
A method for processing cytological specimens comprising: inserting the specimens to be processed into a cup-shaped receptacle having a closed bottom part and closed sidewalls, the closed sidewalls having an upper edge which defines an upper aperture in the receptacle having an area larger than an area of the bottom part, the sidewalls forming an obtuse angle of about 105° to about 135° with the bottom part, the aperture being covered with a perforated membrane for retaining the cytological specimens, wherein the membrane is a mesh having a mesh size up to 80 microns; inserting the receptacle with specimens in a tissue processing apparatus containing processing fluids with the perforated membrane in a vertical position so as to allow an exchange of processing fluids through the membrane and the introduction of paraffin; after processing and removal from the processing apparatus, the receptacle being turned so that the perforated membrane is in a horizontal position and directed upwards, thus permitting the enrichment of the specimens by sedimentation to form an enriched layer at the receptacle bottom; and after allowing to harden to form a paraffin block with the enriched layer at the bottom, the block being removed from the receptacle for histological examination.
Claim 2
Independent Claim
202 words
A method for processing cytological specimens comprising: inserting the specimens to be processed into a cup-shaped receptacle having a closed bottom part and closed sidewalls, the closed sidewalls having an upper edge which defines an upper aperture in the receptacle having an area larger than an area of the bottom part, the sidewalls forming an obtuse angle of about 105° to about 135° with the bottom part, the aperture being covered with a perforated membrane for retaining the cytological specimens, wherein the membrane is a mesh having a mesh size up to 80 microns; inserting the receptacle with specimens in a tissue processing apparatus containing processing fluids with the perforated membrane in a vertical position so as to allow an exchange of processing fluids through the membrane and the introduction of paraffin; after processing and removal from the processing apparatus, the receptacle being turned so that the perforated membrane is in a horizontal position and directed upwards, thus permitting the enrichment of the specimens by sedimentation to form an enriched layer at the receptacle bottom; and after allowing to harden to form a paraffin block with the enriched layer at the bottom, the block being removed from the receptacle for histological examination.
Claim 3
Dependent Claim
12 words
The method of claim 1, wherein the receptacle is of frustoconical shape.
Claim 4
Dependent Claim
25 words
The method of claim 2, wherein the angle between the sidewalls and the bottom part of the receptacle is between about 105° and about 120°.
Claim 5
Dependent Claim
25 words
The method of claim 1, wherein the angle between the sidewalls and the bottom part of the receptacle is between about 105° and about 120°.
Claim 6
Independent Claim
27 words
The method of any one of claims 1, 2, 3 and 4, wherein the membrane has a mesh size of between about 10 and about 80 μm.
Claim 7
Dependent Claim
17 words
The method of claim 5, wherein the mesh size is between about 15 and about 35 μm.
Claim 8
Dependent Claim
13 words
The method of claim 6, wherein the mesh size is about 20 μm.
Claim 9
Dependent Claim
82 words
The method of claim 1, wherein the receptacle includes a means for attaching the membrane to the receptacle, the attaching means comprising a socket positioned at the upper edge of the receptacle and having an opening which mates the aperture in the receptacle and the membrane is incorporated in the opening, and wherein the socket after processing and paraffination of the specimens and removal of the receptacle forms a support for the solidified paraffin block when it is sectioned using a microtome.
Claim 10
Dependent Claim
28 words
The method of claim 8, wherein the receptacle has ridges on its outer sidewalls and the socket is attached to the receptacle by means for engaging the ridges.
Claim 11
Dependent Claim
39 words
The method of claim 8, wherein the opening of the socket has sidewalls above the membrane which form an angle with the membrane which is bigger than the angle between the sidewalls and the bottom part of the receptacle.
Claim 12
Dependent Claim
19 words
The method of claim 1, wherein the receptacle is made of plastic and has a removable plastic bottom part.
References Cited (Backward Citations)
US2996762A
Cited
US3411185A
Cited
US3456300A
Cited
US3982862A
Cited
US4363783A
Cited
US4734372A
Cited
US4557903A
Cited
US4569647A
Cited
US4801553A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-05-16 | 1997-11-15 | 1998-05-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-05-16 | 2001-11-15 | 2002-05-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-05-16 | 2005-11-15 | 2006-05-17 | $7700.00 | Window Closed / Lapsed |