US5284766A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Bed material for cell culture
Assignee: Tokyo Women's Medical University
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Filing: 1992-01-07
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Granted: 1994-02-08
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Expires: 2012-01-07
Abstract
Abstract Disclosed is a bed material whereby cultured or grown cells are collected or detached from the material without a proteolysis enzyme or chemical material. The bed material comprises a support and a coating thereon, wherein the coating is formed from a polymer or copolymer which has a critical solution temperature to water within the range of 0 degrees C. to 80 degrees C.
Inventors
- 👤 Teruo Okano
- 👤 Kazunori Kataoka
- 👤 Noriko Yamada
- 👤 Yasuhisa Sakurai
- 👤 Takayuki Amiya
- 👤 Akira Mamada
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:24
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; C12N2533/00; C12N2533/30
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
117 words
A method for culturing cells, which comprises coating a support with a polymer or copolymer which has a lower or upper critical solution temperature to water within the range of 0° to 80° C., culturing cells attached to the surface thereof at a higher temperature than said lower critical solution temperature or at a lower temperature than said upper critical solution temperature on said coated surface having hydrophobic properties and detaching and collecting the cultured cells from said coated surface by changing the temperature to a lower temperature than said lower critical solution temperature or at a higher temperature than said upper critical solution temperature, thereby changing the properties of said coated surface from hydrophobic to hydrophilic.
Claim 2
Independent Claim
117 words
A method for culturing cells, which comprises coating a support with a polymer or copolymer which has a lower or upper critical solution temperature to water within the range of 0° to 80° C., culturing cells attached to the surface thereof at a higher temperature than said lower critical solution temperature or at a lower temperature than said upper critical solution temperature on said coated surface having hydrophobic properties and detaching and collecting the cultured cells from said coated surface by changing the temperature to a lower temperature than said lower critical solution temperature or at a higher temperature than said upper critical solution temperature, thereby changing the properties of said coated surface from hydrophobic to hydrophilic.
Claim 3
Dependent Claim
32 words
A method for culturing cells according to claim 1 wherein said polymer or copolymer has a lower or upper critical solution temperature to water with the range of 0° to 50° C.
Claim 4
Dependent Claim
32 words
A method for culturing cells according to claim 1 wherein said polymer or copolymer has a lower or upper critical solution temperature to water within the range of 20° to 50° C.
Claim 5
Dependent Claim
30 words
A method for culturing cells according to claim 1 wherein said polymer or copolymer is selected from the group consisting of poly(N-isopropyl acrylamide), poly(N-isopropyl methacrylamide), poly(N-n-propyl acrylamide) and poly(N,N-diethyl acrylamide).
Claim 6
Dependent Claim
18 words
A method for culturing cells according to claim 1 wherein said support is prepared from polystyrene or glass.
Claim 7
Dependent Claim
16 words
A method for culturing cells according to claim 1 wherein said support is a Petri dish.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-02-07 | 1997-08-09 | 1998-02-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-02-07 | 2001-08-09 | 2002-02-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-02-07 | 2005-08-09 | 2006-02-08 | $7700.00 | Window Closed / Lapsed |