US5258501A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Stabilization of glycoproteins
Assignee: Individual
•
Filing: 1992-03-02
•
Granted: 1993-11-02
•
Expires: 2012-03-02
Abstract
Abstract A process for the preparation of stabilized glycoproteins without the introduction of foreign molecules. Susceptible monosaccharides covalently attached to the protein part of the molecule undergo periodate oxidation and, after elimination of the remaining periodate, the oxidized glycoprotein is incubated in a buffer under conditions favorable for the reaction between the aldehyde groups generated in the sugar part and the amino acid residues from the protein part. The oxidized carbohydrate chains act as a polyaldehyde crosslinker, with the cross-linking reaction producing intramolecularly and intermolecularly linked derivatives. The amount and size of the intermolecularly linked derivatives are controlled by degree of oxidation and protein concentration. The thermal stability, depends on the degree of oxidation and under optimal conditions is about 10 times better than the stability of native invertase. Additional stabilization of less than optimally oxidized invertase can be achieved by reduction with NaCNBH 3 .
Inventors
- 👤 Slobodan Barbaric
- 👤 Branko Kozulic
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:17
CPC Classifications
C; C07; C07K; C07K1/00; C07K1/107; C07K1/113; C12; C12N; C12N9/00; C12N9/96
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
149 words
A process for the preparation of a stabilized glycoprotein, wherein the glycoprotein is stabilized by intermolecular and/or intramolecular reactions between aldehyde groups in carbohydrate residues of the glycoprotein molecule and active groups of amino acids from the polypeptide moiety of the same glycoprotein, the process comprising the steps of forming the aldehyde groups by periodate oxidation, wherein the periodate oxidation is performed in the absence of any species or reagent able to reduce the aldehyde groups; eliminating iodate and unreacted periodate from the oxidized glycoprotein by a process excluding a reaction with any small molecular weight diol; and incubating the oxidized glycoprotein in the absence of any species or reagent able to react with the aldehyde groups or with amino acid side chains of the glycoprotein in order to effect formation of covalent bonds between the aldehyde groups and the reactive groups of amino acids of the same glycoprotein.
Claim 2
Dependent Claim
149 words
A process for the preparation of a stabilized glycoprotein, wherein the glycoprotein is stabilized by intermolecular and/or intramolecular reactions between aldehyde groups in carbohydrate residues of the glycoprotein molecule and active groups of amino acids from the polypeptide moiety of the same glycoprotein, the process comprising the steps of forming the aldehyde groups by periodate oxidation, wherein the periodate oxidation is performed in the absence of any species or reagent able to reduce the aldehyde groups; eliminating iodate and unreacted periodate from the oxidized glycoprotein by a process excluding a reaction with any small molecular weight diol; and incubating the oxidized glycoprotein in the absence of any species or reagent able to react with the aldehyde groups or with amino acid side chains of the glycoprotein in order to effect formation of covalent bonds between the aldehyde groups and the reactive groups of amino acids of the same glycoprotein.
Claim 3
Dependent Claim
29 words
A process according to claim 1, wherein the molar amount of added periodate is in the range of from 5 to 200% of the monosaccharides attached to the protein.
Claim 4
Dependent Claim
27 words
A process according to claim 2, wherein the periodate oxidation is performed in the dark in a buffered solution with a pH value from 2 to 11.
Claim 5
Dependent Claim
22 words
A process according to claim 2, wherein periodate oxidation is performed for an interval between about 5 minutes and about 24 hours.
Claim 6
Dependent Claim
16 words
A process according to claim 2, wherein periodate oxidation is performed at or below room temperature.
Claim 7
Dependent Claim
21 words
A process according to claim 2, wherein the low molecular weight molecules are eliminated after the periodate oxidation by gel filtration.
Claim 8
Dependent Claim
31 words
A process according to claim 1, wherein the incubation of the oxidized glycoprotein is performed at a pH value equal to a pH value optimal for stability of the unoxidized glycoprotein.
Claim 9
Dependent Claim
28 words
A process according to claim 7, wherein the oxidized glycoprotein is incubated in a solution with a pH value in the range of about 2 to about 11.
Claim 10
Dependent Claim
25 words
A process according to claim 7, wherein the oxidized glycoprotein is incubated at a temperature in the range of about 0° to about 80° C.
Claim 11
Dependent Claim
17 words
A process according to claim 7, wherein the oxidized glycoprotein is not further treated before an application.
Claim 12
Dependent Claim
16 words
A process according to claim 7, wherein the oxidized glycoprotein is further treated before an application.
Claim 13
Dependent Claim
21 words
A process according to claim 11, wherein the further treatment includes a reduction of the bonds formed in the oxidized glycoprotein.
Claim 14
Dependent Claim
20 words
A process according to claim 11, wherein the further treatment comprises a reduction with NaCNBH 3 or NaBH 4 .
Claim 15
Dependent Claim
11 words
A stabilized glycoprotein prepared according to the process of claim 1.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-11-01 | 1997-05-03 | 1997-11-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-11-01 | 2001-05-03 | 2001-11-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-11-01 | 2005-05-03 | 2005-11-02 | $7700.00 | Window Closed / Lapsed |