US5196306A
Expired / Lapsed
US
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Method for the detection or quantitation of an analyte using an analyte dependent enzyme activation system
Assignee: Revvity Health Sciences Inc
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Filing: 1990-09-28
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Granted: 1993-03-23
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Expires: 2010-09-28
Abstract
Abstract The present invention concerns a method to catalyze reporter deposition to improve detection or quantitation of an analyte in a sample by amplifying the detector signal which comprises reacting an analyte dependent enzyme activation system with a conjugate consisting of a detectably labeled substrate specific for the enzyme system, said conjugate reacts with the analyte dependent enzyme activation system to form an activated conjugate which deposits substantially wherever receptor for the activated conjugate is immobilized, said receptor not being reactive with the analyte dependent enzyme activation system. In another embodiment the invention concerns an assay for detecting or quantitating the presence or absence of an analyte in a sample using catalyzed reporter deposition to amplify the reporter signal.
Inventors
- 👤 Mark N. Bobrow
- 👤 Gerald J. Litt
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 05:28
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/535; G01N33/536; G01N33/542; G01N33/58; G01N33/581
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
91 words
A method for the detection or quantitation of an analyte in an assay which comprises using an analyte dependent enzyme activation system comprising at least one enzyme to react with a conjugate consisting of a detectably labeled substrate specific for the enzyme system to form an activated conjugate which deposits substantially wherever at least one receptor for the activated conjugate is immobilized, said receptor not being reactive with the analyte dependent enzyme activation system, wherein deposited detectable labels either directly or indirectly generate a signal which can be detected or quantitated.
Claim 2
Independent Claim
91 words
A method for the detection or quantitation of an analyte in an assay which comprises using an analyte dependent enzyme activation system comprising at least one enzyme to react with a conjugate consisting of a detectably labeled substrate specific for the enzyme system to form an activated conjugate which deposits substantially wherever at least one receptor for the activated conjugate is immobilized, said receptor not being reactive with the analyte dependent enzyme activation system, wherein deposited detectable labels either directly or indirectly generate a signal which can be detected or quantitated.
Claim 3
Dependent Claim
32 words
A method according to claim 1 wherein at least one enzyme of the analyte dependent enzyme activation system is selected from the group consisting of oxidoreductases, hydrolases, lyases, transferases, isomerases, and ligases.
Claim 4
Dependent Claim
22 words
A method according to claim 2 wherein the enzyme is selected from the group consisting of peroxidases, oxidases, phosphatases, esterases and glycosidases.
Claim 5
Dependent Claim
24 words
A method according to claim 3 wherein the enzyme is selected from the group consisting of horseradish peroxidase, glucose oxidase, alkaline phosphatase and beta-galactosidase.
Claim 6
Dependent Claim
12 words
A method according to claim 4 wherein the enzyme is horseradish peroxidase.
Claim 7
Dependent Claim
20 words
A method according to claim 5 wherein the conjugate is selected from the group consisting of biotin-tyramine, p=hydroxyphenylpropionylbiocytin, or fluorescein-tyramine.
Claim 8
Dependent Claim
15 words
A method according to claim 1 wherein the conjugate is reacted with detectably labeled antibody.
Claim 9
Dependent Claim
21 words
A method according to claim 1 wherein the conjugate is reacted with a detectably labeled member of a specific binding pair.
Claim 10
Dependent Claim
15 words
A method according to claim 1 wherein the conjugate is reacted with detectably labeled streptavidin.
Claim 11
Dependent Claim
33 words
A method according to claim 1 wherein the detectable label is selected from the group consisting of enzymes, radioactive isotopes, fluorogenic, chemiluminescent, or electrochemical materials or a member of a specific binding pair.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-03-22 | 1996-09-21 | 1997-03-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-03-22 | 2000-09-21 | 2001-03-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-03-22 | 2004-09-21 | 2005-03-23 | $7700.00 | Window Closed / Lapsed |