US5175255A
Expired / Lapsed
US
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Methods for purification of platelet-derived growth factor
Assignee: Amgen Inc
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Filing: 1987-03-23
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Granted: 1992-12-29
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Expires: 2007-03-23
Abstract
Abstract Monoclonal antibodies specific for epitopes found on the B chain of PDGF (including v-sis, c-sis and platelet-derived forms) may be bound to columns and used for purification of rPDGF B. A solution containing a polypeptide possessing at least part of the structural conformation of rPDGF B is passed over such a column and the rPDGF B is bound to the antibody. The rPDGF B may then be eluted from the column to yield rPDGF B of greater than 95% purity as determined by SDS-PAGE.
Inventors
- 👤 Arlen R. Thomason
- 👤 Margery A. Nicolson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 02:24
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/22; C07K14/00; C07K14/435; C07K14/475; C07K14/49; C07K2319/00; C07K2319/70; C07K2319/74; C07K2319/75; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
69 words
A method for purifying a polypeptide having a sufficient part of the structural conformation of rPDGF B to provide at least one epitope found in rPDGF B c-sis or PDGF B v-sis comprising the steps of: (a) contacting a substrate-bound monoclonal antibody specific for an epitope found in the B chain of PDGF with a solution containing said polypeptide and (b) eluting said polypeptide from said substrate-bound monoclonal antibody.
Claim 2
Independent Claim
69 words
A method for purifying a polypeptide having a sufficient part of the structural conformation of rPDGF B to provide at least one epitope found in rPDGF B c-sis or PDGF B v-sis comprising the steps of: (a) contacting a substrate-bound monoclonal antibody specific for an epitope found in the B chain of PDGF with a solution containing said polypeptide and (b) eluting said polypeptide from said substrate-bound monoclonal antibody.
Claim 3
Dependent Claim
31 words
The method as recited in claim 1 wherein said contacting step comprises the step of passing said solution containing said polypeptide over a column to which is bound said monoclonal antibody.
Claim 4
Dependent Claim
21 words
The method as recited in claim 1 wherein said polypeptide is purified to greater than 95% purity as determined by SDS-PAGE.
Claim 5
Dependent Claim
25 words
The method as recited in claim 3 wherein said monoclonal antibody is a monoclonal antibody expressed by a hybridoma deposited as ATCC No. HB 9356.
Claim 6
Dependent Claim
25 words
The method as recited in claim 3 wherein said monoclonal antibody is a monoclonal antibody expressed by a hybridoma deposited as ATCC No. HB 9357.
Claim 7
Dependent Claim
25 words
The method as recited in claim 3 wherein said monoclonal antibody is a monoclonal antibody expressed by a hybridoma deposited as ATCC No. HB 9369.
Claim 8
Dependent Claim
25 words
The method as recited in claim 3 wherein said monoclonal antibody is a monoclonal antibody expressed by a hybridoma deposited as ATCC No. HB 9371.
Claim 9
Independent Claim
76 words
A method for purifying a polypeptide having a sufficient part of the structural conformation of rPDGF B to provide at least one epitope found in rPDGF B c-sis or PDGF B v-sis comprising the steps of: (a) contacting a substrate-bound monoclonal antibody specific for an epitope found in the B chain of PDGF but not the A chain of PDGF with a solution containing said polypeptide and (b) eluting said polypeptide from said substrate-bound monoclonal antibody.
Claim 10
Independent Claim
76 words
A method for purifying a polypeptide having a sufficient part of the structural conformation of rPDGF B to provide at least one epitope found in rPDGF B c-sis or PDGF B v-sis comprising the steps of: (a) contacting a substrate-bound monoclonal antibody specific for an epitope found in the B chain of PDGF but not the A chain of PDGF with a solution containing said polypeptide and (b) eluting said polypeptide from said substrate-bound monoclonal antibody.
Claim 11
Dependent Claim
31 words
The method as recited in claim 8 wherein said contacting step comprises the step of passing said solution containing said polypeptide over a column to which is bound said monoclonal antibody.
Claim 12
Dependent Claim
21 words
The method as recited in claim 8 wherein said polypeptide is purified to greater than 95% purity as determined by SDS-PAGE.
Claim 13
Dependent Claim
25 words
The method as recited in claim 8 wherein said monoclonal antibody is a monoclonal antibody expressed by a hybridoma deposited as ATCC No. HB 9354.
Claim 14
Dependent Claim
25 words
The method as recited in claim 8 wherein said monoclonal antibody is a monoclonal antibody expressed by a hybridoma deposited as ATCC No. HB 9355.
Claim 15
Dependent Claim
25 words
The method as recited in claim 8 wherein said monoclonal antibody is a monoclonal antibody expressed by a hybridoma deposited as ATCC No. HB 9361.
Claim 16
Dependent Claim
25 words
The method as recited in claim 8 wherein said monoclonal antibody is a monoclonal antibody expressed by a hybridoma deposited as ATCC No. HB 9366.
Claim 17
Dependent Claim
25 words
The method as recited in claim 8 wherein said monoclonal antibody is a monoclonal antibody expressed by a hybridoma deposited as ATCC No. HB 9367.
Claim 18
Dependent Claim
25 words
The method as recited in claim 8 wherein said monoclonal antibody is a monoclonal antibody expressed by a hybridoma deposited as ATCC No. HB 9368.
Claim 19
Dependent Claim
25 words
The method as recited in claim 8 wherein said monoclonal antibody is a monoclonal antibody expressed by a hybridoma deposited as ATCC No. HB 9370.
Claim 20
Dependent Claim
25 words
The method as recited in claim 8 wherein said monoclonal antibody is a monoclonal antibody expressed by a hybridoma deposited as ATCC No. HB 9372.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-12-29 | 1996-06-29 | 1996-12-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-12-29 | 2000-06-29 | 2000-12-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-12-29 | 2004-06-29 | 2004-12-29 | $7700.00 | Window Closed / Lapsed |