US5166059A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Gene therapy using gene fusions for genetic or acquired disorders
Assignee: United States Department of Health and Human Services
•
Filing: 1991-05-03
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Granted: 1992-11-24
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Expires: 2011-05-03
Abstract
Abstract Gene therapy utilizing an MDR1 linked fusion coding sequence has been disclosed. ADA activity has been introduced into cells using MDR1 linked fusion gene.
Inventors
- 👤 Ira Pastan
- 👤 Michael Gottesman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 04:26
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/78; A; A01; A01K; A01K67/00; A01K67/027; A01K67/0271; C07; C07K; C07K14/00; C07K14/435; C07K14/705; C12N15/00; C12N15/09; C12N15/11; C12N15/62; C12N15/63; C12N15/79; C12N15/85; A61; A61K; A61K48/00; C07K2319/00; C12N2799/00; C12N2799/02; C12N2799/021; C12N2799/027
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
42 words
A nucleotide sequence encoding an MDR1-ADA fusion gene comprising the coding sequence for the human MDR1 gene directly linked to the 5' end of the coding sequence for the ADA gene, wherein the linked coding sequences form a single open reading frame.
Claim 2
Dependent Claim
42 words
A nucleotide sequence encoding an MDR1-ADA fusion gene comprising the coding sequence for the human MDR1 gene directly linked to the 5' end of the coding sequence for the ADA gene, wherein the linked coding sequences form a single open reading frame.
Claim 3
Dependent Claim
10 words
An expression vector comprising the fusion gene of claim 1.
Claim 4
Dependent Claim
10 words
An expression vector having the identifying characteristics of ATCC 67699.
Claim 5
Dependent Claim
10 words
An expression vector having the identifying characteristics of ATCC 67699.
Claim 6
Dependent Claim
14 words
The expression vector of claim 2, wherein said expression vector is a retroviral vector.
Claim 7
Dependent Claim
28 words
The expression vector of claim 4 wherein said vector further comprises the Harvey sarcoma viral LTR promoter operably linked to the nucleotide sequence encoding the MDR1-ADA fusion protein.
Claim 8
Dependent Claim
10 words
A mammalian cell transfected with the vector of claim 2.
Claim 9
Dependent Claim
31 words
A method for expression the MDR1-ADA fusion gene which comprises culturing the mammalian cells of claim 6 under suitable conditions so that the MDR1-ADA fusion protein is produced by said cells.
Claim 10
Dependent Claim
28 words
The method of claim 7 wherein said cells are cultured in the presence of a stimulatory drug under conditions that result in amplification of the MDR1-ADA fusion protein.
References Cited (Backward Citations)
US4704362A
Cited
EP0174810A2
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-11-24 | 1996-05-25 | 1996-11-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-11-24 | 2000-05-25 | 2000-11-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-11-24 | 2004-05-25 | 2004-11-24 | $7700.00 | Window Closed / Lapsed |