US5045446A
Expired / Lapsed
US
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Lyophilization of cells
Assignee: Terumo BCT Inc
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Filing: 1989-07-11
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Granted: 1991-09-03
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Expires: 2009-07-11
Abstract
Abstract A process and medium are disclosed for the lyophilization of cells which comprises the use of solutions including monosaccharide hexoses and pentoses, and/or biocompatible amphipathic polymers to permit the reconstitution of viable cells.
Inventors
- 👤 Raymond P. Goodrich, Jr.
- 👤 Christine M. Williams
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-22 23:43
CPC Classifications
A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/125
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
57 words
A process for the lyophilization of cells having a cell membrane, comprising immersing a plurality of cells in a water solution which includes a monosaccharide, which is capable of permeating the membrane of the cells, in a concentration of from about 7.0 to 37.5%; freezing the solution; and drying the frozen cells by sublimation of the water.
Claim 2
Dependent Claim
57 words
A process for the lyophilization of cells having a cell membrane, comprising immersing a plurality of cells in a water solution which includes a monosaccharide, which is capable of permeating the membrane of the cells, in a concentration of from about 7.0 to 37.5%; freezing the solution; and drying the frozen cells by sublimation of the water.
Claim 3
Dependent Claim
18 words
The process of claim 1 wherein the monosaccharide is selected from the group consisting of pentoses and hexoses.
Claim 4
Dependent Claim
23 words
The process of claim 1 or 2 wherein the monosaccharide is selected from the group consisting of xylose, glucose, ribose, mannose and fructose.
Claim 5
Dependent Claim
19 words
The process of claim 3 wherein the monosaccharide is present in the solution in a concentration of about 23%.
Claim 6
Dependent Claim
78 words
A process for the lyophilization of cells, comprising: immersing a plurality of cells in a buffered solution which includes: a monosaccharide which is present in the solution in a concentration of from about 7.0 to 37.5%, and a polymer having a molecular weight of from about 1K to about 360K which is present in a concentration of from about 0.7% up to saturation in the solution; freezing the solution; and drying the cells by sublimation of the water.
Claim 7
Dependent Claim
78 words
A process for the lyophilization of cells, comprising: immersing a plurality of cells in a buffered solution which includes: a monosaccharide which is present in the solution in a concentration of from about 7.0 to 37.5%, and a polymer having a molecular weight of from about 1K to about 360K which is present in a concentration of from about 0.7% up to saturation in the solution; freezing the solution; and drying the cells by sublimation of the water.
Claim 8
Dependent Claim
10 words
The process of claim 5 wherein said polymer is amphipathic.
Claim 9
Dependent Claim
21 words
The process of claim 5 wherein said polymer has a molecular weight in the range of about 5K to about 80K.
Claim 10
Dependent Claim
21 words
The process of claim 5, 6 or 7 wherein the monosaccharide is selected from the group consisting of pentoses and hexoses.
Claim 11
Dependent Claim
21 words
The process of claim 8 wherein the monosaccharide is selected from the group consisting of xylose, glucose, ribose, mannose and fructose.
Claim 12
Dependent Claim
18 words
The process of claim 8 wherein the polymer is selected from the group consisting of polyvinylpyrrolidone and dextran.
Claim 13
Dependent Claim
10 words
The process of claim 10 wherein the polymer is polyvinylpyrrolidone.
Claim 14
Dependent Claim
78 words
A process for the lyophilization of cells, comprising: immersing a plurality of cells in a buffered solution which includes: a monosaccharide which is present in the solution in a concentration of from about 7.0 to 37.5%, and a polymer having a molecular weight of from about 1K to about 360K which is present in a concentration of from about 0.7% up to saturation in the solution; freezing the solution; and drying the cells by sublimation of the water.
Claim 15
Dependent Claim
78 words
A process for the lyophilization of cells, comprising: immersing a plurality of cells in a buffered solution which includes: a monosaccharide which is present in the solution in a concentration of from about 7.0 to 37.5%, and a polymer having a molecular weight of from about 1K to about 360K which is present in a concentration of from about 0.7% up to saturation in the solution; freezing the solution; and drying the cells by sublimation of the water.
Claim 16
Dependent Claim
10 words
The process of claim 12 wherein said polymer is amphipathic.
Claim 17
Dependent Claim
21 words
The process of claim 12 wherein said polymer has a molecular weight in the range of about 5K to about 80K.
Claim 18
Dependent Claim
21 words
The process of claim 12, 13 or 14 wherein the monosaccharide is selected from the group consisting of pentoses and hexoses.
Claim 19
Dependent Claim
21 words
The process of claim 15 wherein the monosaccharide is selected from the group consisting of xylose, glucose, ribose, mannose and fructose.
Claim 20
Dependent Claim
18 words
The process of claim 15 wherein the polymer is selected from the group consisting of polyvinylpyrrolidone and dextran.
Claim 21
Dependent Claim
10 words
The process of claim 17 wherein the polymer is polyvinylpyrrolidone.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1994-09-02 | 1995-03-04 | 1995-09-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-09-02 | 1999-03-04 | 1999-09-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-09-02 | 2003-03-04 | 2003-09-03 | $7700.00 | Window Closed / Lapsed |