Patent EP0420996A1 successfully refreshed from Google Patents.
US5043265A
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Inorganic phosphor labelled macromolecules; a process for their preparation and their use for immunological or immunocytochemical assays
Assignee: RIJKSUNIVERSITEIT LEIDEN STATIONSWEG 46 2312 AV LEIDEN NETHERLANDS
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Filing: 1986-08-04
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Granted: 1991-08-27
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Expires: 2006-08-04
Abstract
Abstract The invention relates to macromolecules, such as proteins, including immunologically specific antibodies, lipoproteins, polynucleotides, etc., provided with luminescent labels. The invention further relates to a process for preparing macromolecules, such as proteins, including immunologically specific antibodies, lipoproteins, polynucleotides, etc., provided with luminescent labels, and also to the use of labelled immunologically specific macromolecules, such as antibodies, for immunological or immunocytochemical assays.
Inventors
- 👤 Hendrikus J. Tanke
- 👤 Johannes C. Slats
- 👤 Johan S. Ploem
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:43
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533; B; B82; B82Y; B82Y15/00; G01N33/58; G01N33/582; G01N33/588; Y; Y10; Y10S; Y10S435/00; Y10S435/973; Y10S436/00; Y10S436/80; Y10S436/805
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
74 words
An aqueous stabilized suspension of inorganic crystalline phosphor particles having surfaces provided with charged groups and a particle size of 5 um or less, said inorganic crystalline phosphor particles carrying a macromolecular biological substance selected from the group consisting of immunoglobulins, lipoproteins and polynucleotides, said macromolecular biological substance being bound either covalently or by physical adsorption to said inorganic crystalline phosphor particles which can function as a luminescent label of said macromolecular biological substance.
Claim 2
Dependent Claim
74 words
An aqueous stabilized suspension of inorganic crystalline phosphor particles having surfaces provided with charged groups and a particle size of 5 um or less, said inorganic crystalline phosphor particles carrying a macromolecular biological substance selected from the group consisting of immunoglobulins, lipoproteins and polynucleotides, said macromolecular biological substance being bound either covalently or by physical adsorption to said inorganic crystalline phosphor particles which can function as a luminescent label of said macromolecular biological substance.
Claim 3
Dependent Claim
23 words
An aqueous stabilized suspension as claimed in claim 1 in which the phosphor label has a particle size of 1 um or less.
Claim 4
Dependent Claim
23 words
An aqueous stabilized suspension as claimed in claim 1 in which the phosphor has a particle size of 0.02 um to 5 um.
Claim 5
Dependent Claim
81 words
A process for preparing an aqueous stabilized suspension of inorganic crystalline phosphor particles having surfaces provided with charged groups and a particle size of 5 um or less said inorganic crystalline phosphor particles carrying a macromolecular biological substance selected from the group consisting of immunoglobulins, lipoproteins and polynucleotides comprising the step of binding said macromolecular biological substance either covalently or by physical adsorption to said inorganic crystalline phosphor particles which can function as a luminescent label of said macromolecular biological substance.
Claim 6
Dependent Claim
81 words
A process for preparing an aqueous stabilized suspension of inorganic crystalline phosphor particles having surfaces provided with charged groups and a particle size of 5 um or less said inorganic crystalline phosphor particles carrying a macromolecular biological substance selected from the group consisting of immunoglobulins, lipoproteins and polynucleotides comprising the step of binding said macromolecular biological substance either covalently or by physical adsorption to said inorganic crystalline phosphor particles which can function as a luminescent label of said macromolecular biological substance.
Claim 7
Dependent Claim
20 words
A process according to claim 4 in which the phosphor label has a particle size of 1 um or less.
Claim 8
Dependent Claim
20 words
A process according to claim 4 in which the phosphor label has a particles size of 0.02 to 5 um.
Claim 9
Dependent Claim
21 words
A process as claimed in claim 4 in which the macromolecular substance is bonded to a crystalline phosphor by physical adsorption.
Claim 10
Dependent Claim
26 words
A process according to claim 7 in which the bonding is realized at a pH in the vicinity of the isoelectric point of the macromolecular substance.
Claim 11
Dependent Claim
22 words
A process as claimed in claim 4 in which the macromolecular substance is covalently bonded to the crystalline phosphors via spacer molecules.
Claim 12
Dependent Claim
25 words
A process as claimed in claim 9 in which the bonding is realized via a spacer of E-aminocapronic acid introduced by means of water-soluble carbodiimide.
Claim 13
Independent Claim
106 words
A method for the immunological or immunocytochemical detection of a chemical substance which can be bound specifically by antibodies, said method comprising the steps of (1) contacting a sample possibly containing said substance with an aqueous stabilized suspension of inorganic crystalline phosphor particles having surfaces provided with charged groups and a particle size of 5 um or less, said inorganic crystalline phosphor particles carrying specific antibodies bound either covalently or by physical adsorption to said crystalline phosphor particles which function as a luminescent label of said antibodies, and (2) detecting the luminescent label which is indicative of the presence of said antibodies bound to said substance.
Claim 14
Independent Claim
106 words
A method for the immunological or immunocytochemical detection of a chemical substance which can be bound specifically by antibodies, said method comprising the steps of (1) contacting a sample possibly containing said substance with an aqueous stabilized suspension of inorganic crystalline phosphor particles having surfaces provided with charged groups and a particle size of 5 um or less, said inorganic crystalline phosphor particles carrying specific antibodies bound either covalently or by physical adsorption to said crystalline phosphor particles which function as a luminescent label of said antibodies, and (2) detecting the luminescent label which is indicative of the presence of said antibodies bound to said substance.
Claim 15
Dependent Claim
29 words
The method as claimed in claim 11 wherein the detecting is accomplished by fluorescence microscopy or flow cytometry with UV excitation or by scanning electron microscopy with electron excitation.
Claim 16
Dependent Claim
16 words
The method as claimed in claim 11 wherein the detecting is accomplished by time-resolved luminescence assays.
Claim 17
Independent Claim
225 words
A method for the simultaneous detection of at least two different antigens in a biological sample, comprising the steps of (1) treating said sample either simultaneously or consecutively with a first aqueous stabilized suspension of first inorganic crystalline phosphor particles having surfaces provided with charged groups and a particle size of 5 um or less, said inorganic crystalline phosphor particles carrying a first specific antibody capable of binding specifically to one of the antigens to be detected and being bound either covalently or by physical adsorption to said first inorganic crystalline phosphor particles which function as a luminescent label of said first specific antibody and with at least one second aqueous stabilized suspension of second inorganic crystalline phosphor particles having surfaces provided with charged groups and a particle size of 5 um or less, said inorganic crystalline phosphor particles carrying a second specific antibody capable of binding specifically to another of the antigens to be detected and being bound either covalently or by physical adsorption to said second inorganic crystalline phosphor particles which function as a luminescent label of said second specific antibody, and (2) detecting the respective luminescent labels which can be distinguished on the basis of their mutually different spectral characteristics or luminescence decay times and are each indicative of the presence of antibody bound to one of the antigens to be detected.
Claim 18
Independent Claim
225 words
A method for the simultaneous detection of at least two different antigens in a biological sample, comprising the steps of (1) treating said sample either simultaneously or consecutively with a first aqueous stabilized suspension of first inorganic crystalline phosphor particles having surfaces provided with charged groups and a particle size of 5 um or less, said inorganic crystalline phosphor particles carrying a first specific antibody capable of binding specifically to one of the antigens to be detected and being bound either covalently or by physical adsorption to said first inorganic crystalline phosphor particles which function as a luminescent label of said first specific antibody and with at least one second aqueous stabilized suspension of second inorganic crystalline phosphor particles having surfaces provided with charged groups and a particle size of 5 um or less, said inorganic crystalline phosphor particles carrying a second specific antibody capable of binding specifically to another of the antigens to be detected and being bound either covalently or by physical adsorption to said second inorganic crystalline phosphor particles which function as a luminescent label of said second specific antibody, and (2) detecting the respective luminescent labels which can be distinguished on the basis of their mutually different spectral characteristics or luminescence decay times and are each indicative of the presence of antibody bound to one of the antigens to be detected.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-08-26 | 1995-02-25 | 1995-08-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-08-26 | 1999-02-25 | 1999-08-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-08-26 | 2003-02-25 | 2003-08-27 | $7700.00 | Window Closed / Lapsed |