Abstract A disposable, inexpensive electrode for an electrochemical sensor includes a laminated anode and a laminated cathode. The anode is a film substrate with anodic material such as platinum deposited on the substrate. The cathode is also a film substrate with cathodic material such as silver deposited on the substrate. The anode and cathode can be laminated with an intermediate layer of dielectric material. A working surface is provided on the electrode and defined, in part, by the anode and cathode. One procedure for defining the working surface is to fabricate spaced openings in the cathode film and the layer of dielectric material. The openings in the cathode film and dielectrical layer are aligned and the exposed surfaces of the anode and cathode define the working area. An enzyme, such as glucose oxidase, is bonded to the exposed anode and a membrane is applied over the working area and enzyme. The membrane is preferably a silicone water-based elastomer. The laminated anode, cathode and dielectric layer are then severed to form individual electrodes each including a working area.
A method of fabricating an electrode, comprising the steps of: fabricating an opening in a layer of cathodic material; fabricating an opening in a layer of dielectric material; laminating said layer of cathodic material to one side of said layer of dielectric material and laminating a layer of anodic material to the opposite side of said layer of dielectric material such that the opening in the layer of dielectric material and the opening in the layer of cathodic material are aligned to form a working area; inserting glucose oxidase in said working area onto at least a portion of said layer of anodic material; and applying a glucose permeable, silicon water-based elastomer over said glucose oxidase in the aligned openings forming said working area.
Claim 2
Independent Claim
124 words
A method of fabricating an electrode, comprising the steps of: fabricating an opening in a layer of cathodic material; fabricating an opening in a layer of dielectric material; laminating said layer of cathodic material to one side of said layer of dielectric material and laminating a layer of anodic material to the opposite side of said layer of dielectric material such that the opening in the layer of dielectric material and the opening in the layer of cathodic material are aligned to form a working area; inserting glucose oxidase in said working area onto at least a portion of said layer of anodic material; and applying a glucose permeable, silicon water-based elastomer over said glucose oxidase in the aligned openings forming said working area.
Claim 3
Dependent Claim
35 words
The method of claim 1 in which the layer of cathodic material, the layer of dielectric material and the layer of anodic material are severed to form an individual electrode following lamination of said layers.
Claim 4
Dependent Claim
26 words
The method of claim 1 in which the glucose permeable, silicon water-based elastomer is cured after being applied over the glucose oxidase in said working area.
Claim 5
Dependent Claim
27 words
The method of claim 1 in which the layer of cathodic material and the layer of anodic material are laminated to the layer of dielectric material simultaneously.
Claim 6
Dependent Claim
25 words
The method of claim 1 which further includes connecting separate electrical connectors to said layer of cathodic material and to said layer of anodic material.