US5016644A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Urine testing module and method of collecting urine antigen
Assignee: La Mina Ltd
•
Filing: 1989-09-25
•
Granted: 1991-05-21
•
Expires: 2009-09-25
Abstract
Abstract A method for testing for biological molecular indicators in urine. Urine is transported through a sample container under pressure to flow through the sample container so that antigens in the urine are collected and bound on antibodies immobilized on the beads to form antigen-antibody complex. The beads are washed to remove cell debris and charged molecules and a specific prelabelled antibody solution is passed through the sample container with the prelabelled antibodies attaching to a receptor site on the captured antigen to form an antibody-antigen-prelabelled antibody sandwich complex. This sandwich complex is washed to remove cell debris and charged molecules and mixed with a color reagent solution which reacts with the prelabelled antibody to produce an color indicating the presence of a specific cancer antigen.
Inventors
- 👤 Raouf A. Guirguis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:36
CPC Classifications
B; B01; B01L; B01L3/00; B01L3/50; B01L3/508; B01L3/5082; A; A61; A61B; A61B10/00; A61B10/0045; A61B10/0096; B01L3/502; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/80; A61B10/007; A61B2010/0003; A61B2010/0006; A61B5/00; A61B5/15; A61B5/150007; A61B5/150015; A61B5/15003; A61B5/150206; A61B5/150236; A61B5/150244; A61B5/153
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
124 words
A method of testing for the presence of cancer antigen comprising the steps of: a. collecting urine into an apparatus for collecting biological fluids; b. passing the urine through a urine treatment container holding an immobilized antibody bead means to capture antigen from the urine; c. washing the bead means antibody-antigen complex formed in step b to remove non bound materials, d. mixing the bead means antibody complex with a prelabelled primary antibody solution to form a sandwich complex of antibody antigen prelabelled primary antibody; e. washing the sandwich complex bead means to remove non bound materials; and f. immersing the washed sandwich bead means in a coloring reagent which reacts with the prelabelled primary antibody to produce a color which indicates cancer presence.
Claim 2
Independent Claim
124 words
A method of testing for the presence of cancer antigen comprising the steps of: a. collecting urine into an apparatus for collecting biological fluids; b. passing the urine through a urine treatment container holding an immobilized antibody bead means to capture antigen from the urine; c. washing the bead means antibody-antigen complex formed in step b to remove non bound materials, d. mixing the bead means antibody complex with a prelabelled primary antibody solution to form a sandwich complex of antibody antigen prelabelled primary antibody; e. washing the sandwich complex bead means to remove non bound materials; and f. immersing the washed sandwich bead means in a coloring reagent which reacts with the prelabelled primary antibody to produce a color which indicates cancer presence.
Claim 3
Dependent Claim
17 words
A method as claimed in claim 1 including repeating steps b and d a plurality of times.
Claim 4
Independent Claim
132 words
A method of testing for predetermined antigen in urine comprising the steps of: a. placing an antigen collecting apparatus in fluid communication with urine and causing the urine to pass through the antigen collecting apparatus so that antigens are captured by antibody bead means located in said collecting apparatus forming an antigen-antibody complex bead means; b. washing the antigen-antibody complex bead means to remove non bound debris, c. causing a prelabelled primary antibody solution to contact the antigen-antibody complex bead means to form an antigen-antibody sandwich complex with the prelabelled primary antibody being bound to the antigen of the antigen-antibody complex; d. washing the sandwich complex to remove non bound debris; and e. mixing the sandwich complex in a reactant solution to form an indicator indicating the presence of a specific cancer.
Claim 5
Independent Claim
132 words
A method of testing for predetermined antigen in urine comprising the steps of: a. placing an antigen collecting apparatus in fluid communication with urine and causing the urine to pass through the antigen collecting apparatus so that antigens are captured by antibody bead means located in said collecting apparatus forming an antigen-antibody complex bead means; b. washing the antigen-antibody complex bead means to remove non bound debris, c. causing a prelabelled primary antibody solution to contact the antigen-antibody complex bead means to form an antigen-antibody sandwich complex with the prelabelled primary antibody being bound to the antigen of the antigen-antibody complex; d. washing the sandwich complex to remove non bound debris; and e. mixing the sandwich complex in a reactant solution to form an indicator indicating the presence of a specific cancer.
Claim 6
Dependent Claim
17 words
The method as claimed in claim 3 including repeating steps a and c at least one time.
Claim 7
Dependent Claim
15 words
The method as claimed in claim 3 including repeating steps a and c three times.
Claim 8
Dependent Claim
17 words
The method as claimed in claim 3 including repeating steps a and c a plurality of times.
Claim 9
Dependent Claim
27 words
The method as claimed in claim 3 including the step of mixing said urine with a buffering solution before causing urine flow through the antibody bead means.
Claim 10
Independent Claim
131 words
A method of collecting antigen from urine for testing for cancer comprising the steps of: a. mixing a buffer solution in urine; b. withdrawing the buffered urine into a syringe means containing a filter and beads with immobilized antibodies to obtain a concentration of antigen on said beads with immobilized antibodies; c. washing the complexed antigen-antibody beads to remove the stray ligands and debris; d. mixing the antigen-antibody complex beads with a prelabelled primary antibody solution to form a sandwich complex with the prelabelled primary antibody fastening on to the captured antigen receptor sites; e. washing the sandwich antigen-antibody complex to remove stray ligands and debris; and f. treating the washed sandwich complex with a coloring reagent to obtain a colored solution indicating the presence of a specific antigen cancer marker.
Claim 11
Independent Claim
131 words
A method of collecting antigen from urine for testing for cancer comprising the steps of: a. mixing a buffer solution in urine; b. withdrawing the buffered urine into a syringe means containing a filter and beads with immobilized antibodies to obtain a concentration of antigen on said beads with immobilized antibodies; c. washing the complexed antigen-antibody beads to remove the stray ligands and debris; d. mixing the antigen-antibody complex beads with a prelabelled primary antibody solution to form a sandwich complex with the prelabelled primary antibody fastening on to the captured antigen receptor sites; e. washing the sandwich antigen-antibody complex to remove stray ligands and debris; and f. treating the washed sandwich complex with a coloring reagent to obtain a colored solution indicating the presence of a specific antigen cancer marker.
Claim 12
Dependent Claim
18 words
The method as claimed in claim 8 including the step of adding another solution to said reactant solution.
Claim 13
Dependent Claim
19 words
The method as claimed in claim 9 wherein said another solution added to said reactant solution is hydrogen peroxide.
Claim 14
Dependent Claim
16 words
The method as claimed in claim 8 wherein said prelabelled primary antibody is labelled with HRP.
Claim 15
Dependent Claim
13 words
The method as claimed in claim 8 wherein said reactant solution is ABTS.
Claim 16
Dependent Claim
13 words
The method as claimed in claim 8 wherein said reactant solution is TMB.
Claim 17
Dependent Claim
13 words
The method as claimed in claim 8 wherein said reactant solution is OPD.
References Cited (Backward Citations)
US3312221A
Cited
US4473530A
Cited
US4492258A
Cited
US4661100A
Cited
US4685472A
Cited
WO1989002914A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-05-20 | 1994-11-19 | 1995-05-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-05-20 | 1998-11-19 | 1999-05-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-05-20 | 2002-11-19 | 2003-05-21 | $7700.00 | Window Closed / Lapsed |