US5010183A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for purifying DNA and RNA using cationic detergents
Assignee: Qiagen NV
•
Filing: 1989-07-07
•
Granted: 1991-04-23
•
Expires: 2009-07-07
Abstract
Abstract A novel method is provided for purifying DNA and RNA from a variety of sources, including cells, cell lysates, viruses, tissues, blood and other body fluids employing a cationic detergent to complex with the nucleic acids.
Inventors
- 👤 Donald E. Macfarlane
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:59
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1003; C12Q; C12Q1/00; C12Q1/68; C12Q1/6806
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
57 words
A process for the purification of DNA and RNA from a biological mixture consisting essentially of applying directly to said mixture without prior purification or extraction of the nucleic acids a selected quaternary amine cationic detergent capable of simultaneously solubilizing contaminants in said mixture and forming a hydrophobic complex with said DNA or RNA in said mixture.
Claim 2
Dependent Claim
57 words
A process for the purification of DNA and RNA from a biological mixture consisting essentially of applying directly to said mixture without prior purification or extraction of the nucleic acids a selected quaternary amine cationic detergent capable of simultaneously solubilizing contaminants in said mixture and forming a hydrophobic complex with said DNA or RNA in said mixture.
Claim 3
Dependent Claim
62 words
A proceeds for the purification of DNA and RNA from a biological mixture containing cells, lysed or intact, consisting essentially of applying directly to said mixture without prior purification or extraction of the nucleic acids a selected quaternary amine cationic detergent capable of simultaneously solubilizing contaminants in said mixture and forming a hydrophobic complex with said DNA or RNA in said mixture.
Claim 4
Dependent Claim
62 words
A proceeds for the purification of DNA and RNA from a biological mixture containing cells, lysed or intact, consisting essentially of applying directly to said mixture without prior purification or extraction of the nucleic acids a selected quaternary amine cationic detergent capable of simultaneously solubilizing contaminants in said mixture and forming a hydrophobic complex with said DNA or RNA in said mixture.
Claim 5
Dependent Claim
59 words
A process for the purification of DNA and RNA from a biological mixture containing virus consisting essentially of applying directly to said mixture without prior purification or extraction of the nucleic acids of selected quaternary amine cationic detergent capable of simultaneously solubilizing contaminants in said mixture and forming a hydrophobic complex with said DNA or RNA in said mixture.
Claim 6
Dependent Claim
59 words
A process for the purification of DNA and RNA from a biological mixture containing virus consisting essentially of applying directly to said mixture without prior purification or extraction of the nucleic acids of selected quaternary amine cationic detergent capable of simultaneously solubilizing contaminants in said mixture and forming a hydrophobic complex with said DNA or RNA in said mixture.
Claim 7
Dependent Claim
17 words
The process according to claim 1 further consisting essentially of separating said hydrophobic complex from said contaminants.
Claim 8
Dependent Claim
30 words
The process according to claim 4 wherein said separating step consists essentially of centrifuging said mixture following the addition of fine particles of matter for facilitating precipitation of said complex.
Claim 9
Dependent Claim
26 words
The process according to claim 4 wherein said separating step consists essentially of collecting said complex on a surface or a matrix by filtration or absorption.
Claim 10
Dependent Claim
24 words
The process according to claim 1 further consisting essentially of dissociating said DNA and RNA from said complex to produce purified DNA and RNA.
Claim 11
Dependent Claim
21 words
The process according to claim 7 wherein said dissociating step comprises treating said complex with a salt in a non-aqueous solvent.
Claim 12
Dependent Claim
13 words
The process according to claim 1 wherein said detergent is an alkylbenzyldimethylammonium salt.
Claim 13
Dependent Claim
26 words
The process according to claim 9 wherein said detergent is an alkylbenzyldimethylammonium chloride, wherein said alkyl group consists essentially of from 10 to 20 carbon atoms.
Claim 14
Dependent Claim
16 words
The process according to claim 10 wherein said alkyl group consists essentially of 16 carbon atoms.
Claim 15
Dependent Claim
17 words
The process according to claim 1 further consisting essentially of adding to said mixture an chaotropic agent.
Claim 16
Dependent Claim
17 words
The process according to claim 1 further consisting essentially of adding to said mixture an sulfhydryl-reducing agent.
Claim 17
Dependent Claim
17 words
The process according to claim 1 further consisting essentially of adding to said mixture an chelating agent.
Claim 18
Dependent Claim
17 words
The process according to claim 1 further consisting essentially of adding to said mixture an non-cationic detergent.
Claim 19
Dependent Claim
16 words
The process according to claim 10 wherein said alkyl group consists essentially of 14 carbon atoms.
Claim 20
Dependent Claim
16 words
The process according to claim 10 wherein said alkyl group consists essentially of 18 carbon atoms.
Claim 21
Dependent Claim
28 words
The process according to claim 1 wherein said biological material consists essentially intact cells, cell lysates, blood, viruses, or other body fluids or solutions containing DNA or RNA.
Claim 22
Dependent Claim
45 words
A kit for isolating nucleic acids from a sample biological mixture containing nucleic acids in contaminating material consisting essentially of a cationic detergent in an amount sufficient to solubilize contaminants in said sample and form a hydrophobic complex with nucleic acids present in said sample.
Claim 23
Dependent Claim
45 words
A kit for isolating nucleic acids from a sample biological mixture containing nucleic acids in contaminating material consisting essentially of a cationic detergent in an amount sufficient to solubilize contaminants in said sample and form a hydrophobic complex with nucleic acids present in said sample.
Claim 24
Dependent Claim
15 words
The kit according to claim 19 wherein said detergent using a quaternary amine cationic detergent.
Claim 25
Dependent Claim
13 words
The kit according to claim 19 wherein said detergent is an alkylbenzyldimethylammonium salt.
Claim 26
Dependent Claim
14 words
The kit according to claim 21 wherein said detergent is an alkyl benzyldimethylammonium chloride.
Claim 27
Dependent Claim
19 words
The kit according to claim 22 wherein said alkyl group consists essentially of from 10 to 20 carbon atoms.
Claim 28
Dependent Claim
16 words
The kit according to claim 23 wherein said alkyl group consists essentially of 16 carbon atoms.
References Cited (Backward Citations)
US3152116A
Cited
US3157637A
Cited
US3845033A
Cited
US4018916A
Cited
US4833239A
Cited
US4843155A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-04-22 | 1994-10-22 | 1995-04-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-04-22 | 1998-10-22 | 1999-04-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-04-22 | 2002-10-22 | 2003-04-23 | $7700.00 | Window Closed / Lapsed |