US4963263A
Expired / Lapsed
US
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Method of identity analyte-binding peptides
Assignee: Telik Inc
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Filing: 1989-05-16
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Granted: 1990-10-16
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Expires: 2009-05-16
Abstract
Abstract A method for affinity chromatography or adsorption of a designated analyte utilizes a paralog as the affinity partner. The immobilized paralog can be used in purification or analysis of the analyte; the paralog can also be used as a substitute for antibody in an immunoassay. The paralog is identified by screening candidate peptide sequences of 4-20 amino acids for specific affinity to the analyte.
Inventors
- 👤 Lawrence M. Kauvar
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 10:59
CPC Classifications
C; C07; C07K; C07K1/00; C07K1/14; C07K1/16; C07K1/22; B; B01; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/38; B01D15/3804; B01J; B01J20/00; B01J20/30; B01J20/32; B01J20/3202; B01J20/3204; B01J20/3206; B01J20/3208; B01J20/3212; B01J20/3231; B01J20/3242; B01J20/3268; B01J20/3272; B01J20/3274; B01D15/3809; G; G01; G01N; G01N30/00; G01N30/02
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
76 words
A method to identify a peptide having a sequence of 4-20 amino acids useful for the conduct of affinity chromatography with respect to an analyte, wherein said peptide has specific affinity for said analyte, which method comprises: screening, for ability to bind said analyte, a panel of individual candidate peptides wherein said candidate peptides have systematically varied values of at least two parameters selected from the group consisting of hydrophobic index, amphipathic characteristics, and charge pattern.
Claim 2
Independent Claim
76 words
A method to identify a peptide having a sequence of 4-20 amino acids useful for the conduct of affinity chromatography with respect to an analyte, wherein said peptide has specific affinity for said analyte, which method comprises: screening, for ability to bind said analyte, a panel of individual candidate peptides wherein said candidate peptides have systematically varied values of at least two parameters selected from the group consisting of hydrophobic index, amphipathic characteristics, and charge pattern.
Claim 3
Dependent Claim
15 words
The method of claim 1 wherein the candidate peptides have sequences of 5-15 amino acids.
Claim 4
Dependent Claim
46 words
The method of claim 1 wherein said screening step is conducted by assessing the ability of analyte to compete with a labeled peptide mixture, which mixture is capable of binding to all candidate peptides of the panel, for binding to each candidate peptide in the panel.
Claim 5
Dependent Claim
14 words
The method of claim 3 wherein said peptide mixtures are labeled by iodinating acetylation.
Claim 6
Dependent Claim
14 words
The method of claim 1 wherein said two parameters are hydrophobicity and amphipatic characteristics.
Claim 7
Dependent Claim
14 words
The method of claim 1 wherein said two parameters are hydrophobicity and charge pattern.
Claim 8
Dependent Claim
15 words
The method of claim 1 wherein said two parameters are amphiphatic characteristics and charge pattern.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-10-15 | 1994-04-16 | 1994-10-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-10-15 | 1998-04-16 | 1998-10-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-10-15 | 2002-04-16 | 2002-10-16 | $7700.00 | Window Closed / Lapsed |