US4904366A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Instrument for determination of the base sequence of nucleic acid
Assignee: Hitachi Ltd
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Filing: 1987-12-04
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Granted: 1990-02-27
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Expires: 2007-12-04
Abstract
Abstract In the instrument for determination of the base sequence of the present invention, the ionic strength of a buffer solution in a gel is made lower at a detection part than in a region from the detection part towards a negative electrode. Therefore, at the detection part, the electric field intensity can be increased, resulting in a higher migration speed, and hence the electrophoretic pattern can be extended, so that the distance between two adjacent electrophoretic bands can be elongated. Consequently, according to the present invention, the slit width can be narrowed relatively to the electrophoretic pattern without actually narrowing the slit width, and the resolving power can be enhanced without lowering the detection sensitivity.
Inventors
- 👤 Jiro Tokita
- 👤 Keiichi Nagai
- 👤 Tamotu Simada
- 👤 Ken'ichi Watanabe
- 👤 Ryusei Nakano
- 👤 Tomoaki Sumitani
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 08:44
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
149 words
An apparatus for determination of a base sequence of a nucleic acid, comprising: an electrophoretic matrix having a migration lane and buffer therein; means for introducing sample containing nucleic acid fragments into a first end of said electrophoretic matrix; means for applying an electrical potential between positive and negative electrodes for electrophoresis so that a negative potential is applied to said first end of said electrophoretic matrix and a positive potential is applied to a second end of said electrophoresis matrix; detection means located at said migration lane and substantially separated from said first end of said electrophoretic matrix for detecting in real time the nucleic acid fragments successively arriving at a detection part; and the ionic strength of said buffer in said electrophoretic matrix is higher at least at one position in a region spaced from the detection part towards said first end than at the detection part.
Claim 2
Independent Claim
149 words
An apparatus for determination of a base sequence of a nucleic acid, comprising: an electrophoretic matrix having a migration lane and buffer therein; means for introducing sample containing nucleic acid fragments into a first end of said electrophoretic matrix; means for applying an electrical potential between positive and negative electrodes for electrophoresis so that a negative potential is applied to said first end of said electrophoretic matrix and a positive potential is applied to a second end of said electrophoresis matrix; detection means located at said migration lane and substantially separated from said first end of said electrophoretic matrix for detecting in real time the nucleic acid fragments successively arriving at a detection part; and the ionic strength of said buffer in said electrophoretic matrix is higher at least at one position in a region spaced from the detection part towards said first end than at the detection part.
Claim 3
Dependent Claim
53 words
An apparatus for determination of the base sequence of nucleic acid according to claim 1, wherein the concentration of buffer in the electrophoretic matrix is higher at least at said first end position in the region from the detection part towards the negative electrode of the electrophoretic apparatus than at the detection part.
Claim 4
Dependent Claim
36 words
An apparatus according to claim 1, wherein the electrophoretic matrix has a plurality of migration lanes and the detection means are provided such that the nucleic acid fragments on each migration lane can be separately detected.
Claim 5
Dependent Claim
16 words
An apparatus according to claim 1, wherein the nucleic acid fragments are labeled with a radioisotope.
Claim 6
Dependent Claim
15 words
An apparatus according to claim 1, wherein the detection means are for detecting a radiation.
Claim 7
Dependent Claim
36 words
An apparatus according to claim 1, wherein the concentration of the buffer at the detection part is about one-fourth of that at the region from the detection part towards the first end of the electrophoretic matrix.
Claim 8
Dependent Claim
16 words
An apparatus according to claim 1, wherein the electrophoretic matrix is surrounded by the detection means.
Claim 9
Independent Claim
143 words
A method for elongating a migration pattern of nucleic acid fragments when determining a base sequence of nucleic acid comprising: providing a electrophoretic matrix having a migration lane from a first end of a second end and a buffer therein, the ionic strength of said buffer being higher at least at one position in a region between said first end and a detection part separated by a substantial distance from the first end that at said detection part; introducing sample containing nucleic acid fragments into said first end of said electrophoretic matrix; applying voltage between a positive and a negative electrode for electrophoresis with a negative potential applied to said first end of the electrophoretic matrix and a positive potential applied to said second end of said electrophoretic matrix; and detecting in real time the nucleic fragments successively arriving at said detection part.
Claim 10
Independent Claim
143 words
A method for elongating a migration pattern of nucleic acid fragments when determining a base sequence of nucleic acid comprising: providing a electrophoretic matrix having a migration lane from a first end of a second end and a buffer therein, the ionic strength of said buffer being higher at least at one position in a region between said first end and a detection part separated by a substantial distance from the first end that at said detection part; introducing sample containing nucleic acid fragments into said first end of said electrophoretic matrix; applying voltage between a positive and a negative electrode for electrophoresis with a negative potential applied to said first end of the electrophoretic matrix and a positive potential applied to said second end of said electrophoretic matrix; and detecting in real time the nucleic fragments successively arriving at said detection part.
Claim 11
Dependent Claim
46 words
The method according to claim 8 wherein the ionic strength is controlled by making the concentration of the buffer in the electrophoretic matrix higher at least in said first position in the region from the detection part towards the negative electrode than at the detection part.
References Cited (Backward Citations)
US4707235A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-02-26 | 1993-08-28 | 1994-02-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-02-26 | 1997-08-28 | 1998-02-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-02-26 | 2001-08-28 | 2002-02-27 | $7700.00 | Window Closed / Lapsed |