US4895955A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Non-radioactive carbodiimide precursor to nucleic acid probes
Assignee: LIFECODES Corp OLD SAW MILL RIVER RD VALHALLA NEW YORK 10595 A CORP OF NY
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Filing: 1988-05-27
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Granted: 1990-01-23
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Expires: 2008-05-27
Abstract
Abstract This invention relates to carbodiimide compounds of the formula: Z--L--W--N═C═N--R.sub.1 wherein Z is a signalling moiety or a protecting group; L is a divalent linking group; W is an alkylene group having up to 24 carbon atoms which can be substituted or interrupted by a water solubility enhancing group or cleavable S--S group; and R 1 is an alkyl group having up to 18 carbon atoms. These compounds are useful in the nonradioactive labelling of nucleic acid probes, and methods employing same.
Inventors
- 👤 John P. Ford
- 👤 Charles W. Blewett
- 👤 Michael D. Sublett
- 👤 Bernard F. Erlanger
- 👤 Alan F. Cook
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:18
CPC Classifications
C; C07; C07D; C07D495/00; C07D495/02; C07D495/04; C07H; C07H21/00; Y; Y02; Y02P; Y02P20/00; Y02P20/50; Y02P20/55
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
61 words
A carbodiimide compound of the Formula I: Z--L--W--N═C═N--R.sub.1 wherein Z is a biotin group; L is a divalent linking group; W is an alkylene group of up to 24 carbon atoms which can be substituted with or interrupted by a water solubility enhancing group or cleavable S--S group; R 1 is alkyl or substituted alkyl of up to 18 carbon atoms.
Claim 2
Dependent Claim
61 words
A carbodiimide compound of the Formula I: Z--L--W--N═C═N--R.sub.1 wherein Z is a biotin group; L is a divalent linking group; W is an alkylene group of up to 24 carbon atoms which can be substituted with or interrupted by a water solubility enhancing group or cleavable S--S group; R 1 is alkyl or substituted alkyl of up to 18 carbon atoms.
Claim 3
Dependent Claim
48 words
The compound of claim 1 wherein W is: ##STR10## wherein X 1 and X 2 are each alkylene groups having from 1 to 18 carbon atoms, R 2 and R 3 are each alkyl groups having from 1 to 18 carbon atoms, and Y is a counterbalancing anion.
Claim 4
Dependent Claim
36 words
The compound of claim 2 having the formula: ##STR11## wherein X 1 , X 2 , R 1 and Y have the meanings as in claim 2 and n is an integer from 1 to 3.
Claim 5
Dependent Claim
9 words
The compound of claim 3 having the formula: ##STR12##
References Cited (Backward Citations)
US4751313A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-01-22 | 1993-07-24 | 1994-01-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-01-22 | 1997-07-24 | 1998-01-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-01-22 | 2001-07-24 | 2002-01-23 | $7700.00 | Window Closed / Lapsed |